This paper is about studies on leaf extract of "Napoleona vogelii" on bacterial strains associated with human infections.
"Napoleona vogelii" is most commonly used in herbal preparations to cure wounds, burns, fever, pneumonia, syphilis, and staphylococcal infections. This work was carried out to determine the antimicrobial effectiveness of the extract from "Napoleona vogelii" leaves on some bacterial strains.
The leaves of "Napoleona vogelii" were collected and air dried until constant weight of the sample was achieved. The sample was grounded into fine powder and extracted using methanol and sterile distilled water solution in the ratio 3:2 (v/v) for 96 hours with regular agitation. The supernatant was filtered and concentrated in vacuo using a rotary evaporator to expel the methanol, and the aqueous part was then lyophilized to obtain the crude extract.
The antimicrobial activity of the crude extract was tested against some selected bacterial strains using agar well diffusion method. Streptomycin was used as positive control. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined using standard methods.
The mean diameter of the zones of inhibition exhibited by the plant extract against the bacterial strains ranged between 13 mm to 17 mm, while the diameter of zones of inhibition exhibited by streptomycin ranged from 14 mm to 25 mm. The MIC value was between 1.09 mg/ml and 17.5 mg/ml while the MBC value ranged between 2.19 mg/ml and 17.5 mg/ml.
The phytochemical screening of the plant extract showed the presence of tannins, saponins, flavonoids, alkaloids, terpenoids, cardiac glycosides, and carbohydrate. In conclusion, the extract obtained from "Napoleona vogelii" leaves exhibited appreciable antibacterial activity against the test organisms and hence can serve as a remedy for bacterial infections.
Inhaltsverzeichnis (Table of Contents)
- CHAPTER ONE
- INTRODUCTION AND LITERATURE REVIEW
- INTRODUCTION
- OBJECTIVES OF THE STUDY
- LITERATURE REVIEW
- MECHANISM OF ACTION OF ANTIMICROBIAL COMPOUNDS
- Inhibition of cell wall synthesis
- Inhibition of Protein synthesis
- Inhibition of nucleic acid synthesis
- Inhibition of intermediary metabolic pathways
- MECHANISMS OF RESISTANCE BY MICROORGANISMS
- Prevention of access to target
- Changes in antibiotic targets by mutation:
- Modification (and protection) of targets:
- Direct modification of antibiotics:
- STUDIES ON SOME ANTIMICROBIAL ACTIVITIES OF SOME MEDICINAL PLANTS
- CHAPTER TWO
- MATERIALS AND METHODS
- MATERIALS
- Microorganisms
- Plant specimen
- Culture media used
- METHODS
- Drying and extraction of plant materials
- Preparation of microorganisms used for the experiment
- Antimicrobial sensitivity testing of crude leaf extract of Napoleona vogelii against some selected bacterial strains.
- Determination of minimum inhibitory concentrations (MICs) of leaf extract of Napoleona vogelii on susceptible organisms.
- Determination of minimum bactericidal concentrations (MBCs) of leaf extracts of Napoleona vogelii on susceptible organisms.
- Phytochemical Analysis
- CHAPTER THREE
- RESULTS
- The crude extract obtained from leaves of Napoleona vogelii.
- The antimicrobial sensitivity testing of leaf extract from Napoleona vogelii against the selected bacterial strains:
- CHAPTER FOUR
- DISCUSSION AND CONCLUSION
- DISCUSSION
- CONCLUSION
- REFERENCES
- APPENDIX
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This dissertation examines the antimicrobial activity of leaf extract from Napoleona vogelii against bacterial strains associated with human infections. The study aims to determine the potential of Napoleona vogelii as a source of natural antimicrobial agents.
- Antimicrobial activity of Napoleona vogelii leaf extract against various bacterial strains.
- Mechanisms of antimicrobial action of the extract.
- Determination of minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the extract.
- Phytochemical analysis of the leaf extract to identify potential bioactive compounds.
- Evaluation of the potential of Napoleona vogelii as a natural antimicrobial agent for treating bacterial infections.
Zusammenfassung der Kapitel (Chapter Summaries)
Chapter One provides an introduction to the research and a comprehensive review of relevant literature. It discusses the prevalence of bacterial infections, the limitations of current antimicrobial therapies, and the growing interest in natural antimicrobial agents. The chapter also explores the mechanism of action of various antimicrobial compounds and the mechanisms by which microorganisms develop resistance to antibiotics. It concludes with a discussion of previous studies on the antimicrobial activity of medicinal plants, highlighting the potential of Napoleona vogelii as a potential source of antimicrobial compounds.
Chapter Two details the materials and methods used in the study. It outlines the bacterial strains, the plant specimen, and the culture media employed. The chapter describes the extraction procedure for the leaf extract and the methods used to evaluate the antimicrobial activity of the extract against the selected bacterial strains. This includes the determination of MICs, MBCs, and phytochemical analysis.
Chapter Three presents the results obtained from the experiments, focusing on the antimicrobial activity of the Napoleona vogelii leaf extract against the bacterial strains and the phytochemical components identified in the extract.
Schlüsselwörter (Keywords)
This research focuses on Napoleona vogelii, antimicrobial activity, bacterial infections, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), phytochemical analysis, medicinal plants, natural antibiotics, and bacterial resistance.
- Quote paper
- Sulaimon Araromi (Author), 2022, Studies on Leaf Extract on Bacterial Strains associated with Human Infections, Munich, GRIN Verlag, https://www.grin.com/document/1243187