Fungal infestation of onion bulbs during storage in sack and on its antioxidant properties

Contents

Abstract

1.0 Introduction
1.1 Background of the Study
1.2 Aim of the Study
1.3 Objectives of the Study

2.0 Literature Review
2.1 Uses of Allium cepa
2.2 Post-harvest losses in onion
2.3 Microorganisms Associated with Onion Spoilage in Nigeria
2.4 Antioxidant effects of A. cepa
2.5 Antimicrobial activity

3.0 Materials and Methods
3.1 Study Area
3.2 Materials used and Sterilization
3.3 Collection of Samples
3.4 Storage of Onion Bulb in Sack Bag
3.5 Preparation and sterilization of media
3.6 Assessment of Fungal Infestation of the Onion bulb during Storage
3.6.1 Preparation of pure cultures of fungal isolates
3.7 Identification of fungal isolates
3.8 Determination of Frequency of Occurrence of Fungal Isolates
3.9 Preparation of Onion Extract
3.10 Determination of antioxidant Properties of Onion
3.10.1 DPPH Free radical scavenging assay
3.10.2 Measurement of reducing power of the crude extracts of Onion Bulb
3.10.3 Determination of antioxidant activity of crude extracts of the Onion
3.11 Statistical Analysis

4.0 Results
4.1 Fungal Infestation of Onion Bulbs during Storage in Sack
4.2 Frequency and Percentages of Fungal Infestation of Onion Bulbs
4.3 DPPH radical scavenging activity of Fungal Infested Onion Bulbs during Storage in Sack

5.0 Discussions and Conclusion
5.1 Discussions
5.2 Conclusion
5.3 Recommendation

References