Phytochemical Screening, Isolation and Antibacterial Evaluation of the Ethyl Acetate Crude Extract of the Root Bark of "Dichapetalum Madagascariense Poir"

Bachelor Thesis , 2019 , 47 Pages , Grade: 4.0 = A

Chemistry - Organic Chemistry

Dichapetalum madagascariense. Poir belongs to the family Dichapetalacease. In this present study, the antibacterial activity of the ethyl acetate extract of the root was investigated by soxhlet extraction process. Agar well diffusion method was employed to evaluate antibacterial activity of extracts against Gram positive and Gram negative bacteria. The root extract was effective in causing inhibition of some of the test bacteria. The highest and least inhibitory activities were observed against Staphylococcus aureus and Pseudomonas aeruginosa respectively, while Escherichia coli was resistant. The extract was more effective in inhibiting the growth of Staphylococcus aureus as compared to the standard antibiotic used as a positive control. The solvent for extraction, ethyl acetate, did not affect the biological activity of the crude extract.

Inhaltsverzeichnis (Table of Contents)

• CHAPTER ONE INTRODUCTION
• 1.1 Background
• 1.2 The Family Dichapetalaceae
• 1.3 Plant Description
• 1.4 Classification of Plant
• 1.5 Plant Distribution
• 1.6 Ethnobotanical Use of Dichapetalum Madagascariense
• 1.7 Local Names of Dichapetalum Madagascariense
• 1.8 Search for New Antimicrobial Agents
• 1.9 Some Bacterial Infections
• 1.9.1 Staphyloccocus Aureus
• 1.9.2 Pseudomonas Aeruginosa
• 1.9.3 Klebsiella Pneumoniae
• 1.9.4 Salmonella Typhi
• 1.10 Statement of Problem
• 1.11 Justification of Study
• 1.12 Aim
• 1.13 Specific Objectives
• CHAPTER TWO LITERATURE REVIEW
• 2.1 Review on Dichapetalum madagacariense
• 2.2 The Dichapetalin
• 2.3 Organic Solvents
• 2.4 Solvents for Extracting Bioactive Compounds
• 2.5 Phytochemicals
• 2.5.1 Saponins
• 2.5.2 Flavonoids
• 2.5.3 Tannins
• 2.6 Examples of Phytochemicals Found in Foods and Their Benefits
• 2.7 Antibacterial Determination
• 2.7.1 Susceptibility Test

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This dissertation focuses on the phytochemical screening, isolation, and antibacterial evaluation of the ethyl acetate crude extract of the root bark of Dichapetalum madagascariense. The study aims to assess the antimicrobial potential of this plant extract against various bacterial strains, including Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella Pneumoniae, and Salmonella Typhi.

• Antibacterial activity of Dichapetalum madagascariense extract
• Phytochemical composition of the plant extract
• Comparison of the extract's efficacy to standard antibiotics
• Potential applications of the extract in medicinal treatments
• Identification of active compounds responsible for antibacterial properties

Zusammenfassung der Kapitel (Chapter Summaries)

Chapter one provides an introduction to the study, discussing the background of Dichapetalum madagascariense and its importance in ethnobotanical medicine. It also explores the search for new antimicrobial agents and the impact of various bacterial infections.

Chapter two delves into a comprehensive literature review, examining previous research on Dichapetalum madagascariense, including its phytochemical constituents and potential pharmacological activities. It also discusses the principles of organic solvents and their role in extracting bioactive compounds from plants. The chapter concludes with a discussion of antibacterial determination methods and their significance in evaluating the efficacy of plant extracts.

Schlüsselwörter (Keywords)

The primary keywords and focus topics of this research are: Dichapetalum madagascariense, antibacterial activity, phytochemical screening, ethyl acetate extract, root bark, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella Pneumoniae, Salmonella Typhi, bioactive compounds, and ethnobotanical medicine.

• College: University of Cape Coast
• Grade: 4.0 = A
• Author: Hannah Bilson (Author)
• Publication Year: 2019
• Pages: 47
• Catalog Number: V1324425
• ISBN (PDF): 9783346815163
• ISBN (Book): 9783346815170
• Language: English
• Tags: phytochemical, screening, isolation, antibacterial, evaluation, ethyl, acetate, crude, extract, root, bark, dichapetalum, madagascariense, poir