Biosurfactant or microbial surfactants are surface dynamic atoms that are delivered by an assortment of microorganisms including microscopic organisms, yeast and filamentous parasite. In contrast with engineered synthetic surfactants, bio-surfactants are of interest because of their high level of activity, particular movement at outrageous temperatures, pH and saltiness, capacity to be delivered from inexhaustible feedstock and high level of biodegradability. In any case, bio surfactants have not yet been marketed widely because of low creation yields and high feedstock and cleansing expenses.
With a specific end goal to tackle this issue many investigations have been done utilizing minimal effort feedstock or farming side-effects as substrates for bio surfactant creation. The foremost extent of the test work was accordingly; to create compelling strategy, and sanitize the biosurfactant generation by waste potato peels and pulp utilizing mutated B.Subtilis DDU20161. Major upstream parameters like creation of medium, fomentation, and pH were kept up in the investigation.
Table of Contents
1. Abstract
2. Introduction
3. Materials and Method
3.1 Microorganism and culture preservation
3.2 Experimental Conditions for the Production of Biosurfactants
3.2.1 Bacterial culture
3.2.2 Minimal Media
3.2.3 Propagation of the Inoculum
3.3 Production of Biosurfactants in a Continuous Stirred Tank Bioreactor
3.4 Characterization of isolated biosurfactant
3.4.1 Biomass Yield
3.4.2 Analysis of amino acid
3.4.3 Biosurfactant Concentration
3.4.4 FT-IR analysis
3.4.5 Surface tension measurement
3.4.6 Emulsification Index (E24)
3.4.7 Foaming activity
3.4.8 TLC study
3.4.9 Determination of Critical Micelle Concentration
3.4.10 Stability study
4. Result and Discussion
5. Conclusion
Research Objectives and Key Topics
The primary aim of this research is to develop a cost-effective and efficient method for the industrial production of biosurfactants using industrial waste, specifically potato peels and pulp, as the carbon source substrate fermentation with mutated Bacillus subtilis DDU20161.
- Utilization of agricultural waste (potato peels) to increase economic viability of biosurfactant production.
- Optimization of fermentation parameters to maximize biosurfactant yields using a mutated Bacillus subtilis strain.
- Comprehensive characterization of the produced biosurfactant using HPLC, FT-IR, and surface tension analysis.
- Evaluation of the thermal and pH stability of the generated biosurfactant for potential commercial applications.
- Reduction of environmental pollution issues associated with industrial potato processing waste.
Excerpt from the Book
Production of Biosurfactants in a Continuous Stirred Tank Bioreactor
The production of biosurfactant was carried out in a 5-L bioreactor (BIOSTAT Cplus; Sartorius) operating with 3 L of available volume, using the culture medium. A concentration of 15% v/v of inoculum (0.95 g/L) was used and the fermentation was conducted at 37°C, 180 rpm and with 1 L/min aeration. The samples were collected at pre-defined intervals to determine biomass and later centrifuged at 8000 rpm for 20 minutes at 4 °C to remove the cells. Clear supernatant was observed which served as source of crude biosurfactant. Biosurfactant was recovered from the cell free culture supernatant by cold acetone precipitation, ammonium sulphate precipitation and acid precipitation methods8. Equal volume of chilled acetone added to supernatant; allow standing for 10 hrs at 40C. Precipitate was collected by centrifugation and evaporated to dryness to remove residual acetone after which it was re-crystallizing with chloroform and methanol9. The precipitate of biosurfactant was then analyzed for biosurfactant concentration and characterization. Moreover, the biosurfactant was also extracted by using ammonium precipitation and acid precipitation methods for comparision of biosurfactant extractive yields10.
Summary of Chapters
Abstract: Provides a high-level overview of the use of waste potato peels as a substrate for B. Subtilis DDU20161 to produce biosurfactants and summarizes the optimization results.
Introduction: Explains the necessity of green alternatives to synthetic surfactants and the potential of utilizing cheap agricultural byproducts for industrial-scale biosurfactant production.
Materials and Method: Details the micro-organism isolation, culture maintenance, fermentation parameters, and the analytical techniques used to characterize the biosurfactant.
Result and Discussion: Analyzes the experimental data on biomass yield, concentration, spectral analysis via FT-IR and HPLC, and the stability of the biosurfactant under varying conditions.
Conclusion: Summarizes the findings, noting the industrial viability of the developed method and the efficiency of the substrate in producing stable, high-quality biosurfactants.
Keywords
Biosurfactant, B.Subtilis DDU20161, M9 salt solution, Potato pulp, Potato peel, Fermentation, Industrial waste, Lipopeptide, Surfactin, Biomass, Surface activity, Emulsification index, Thermal stability, FT-IR analysis, Chromatographic purification.
Frequently Asked Questions
What is the core focus of this research?
The research focuses on the biosynthesis of biosurfactants using a mutated strain of B. subtilis and waste potato peels, aiming to provide a cost-effective and environmentally friendly production process.
What are the primary thematic areas?
The work covers waste management, biotechnology, microbial fermentation, surfactant purification, and comprehensive chemical characterization.
What is the main research question or goal?
The goal is to determine if potato peels can serve as an efficient, low-cost substrate to maximize biosurfactant yields on a commercial scale while solving local industrial waste problems.
What scientific methods were employed?
The researchers used submerged fermentation in a bioreactor, followed by various analytical techniques including HPLC, FT-IR spectroscopy, thin-layer chromatography, and surface tension measurements.
What does the main body cover?
The main body describes the isolation and preservation of the bacteria, the composition of the minimal media, the fermentation process, and the specific laboratory protocols for product extraction and characterization.
What keywords characterize the study?
Key terms include B. subtilis DDU20161, potato peel substrate, surfactant, fermentation, and bio-industrial application.
Why are potato peels used as a substrate in this study?
Potato peels are a low-cost, abundant agriculture byproduct that contains nutrients and salts capable of supporting bacterial cellular growth and surfactant synthesis, thereby reducing overall production costs.
How stable is the resulting biosurfactant?
The produced biosurfactant demonstrated stability between 30°C and 100°C and maintained surface activity across a pH range of 8 to 12.
What specific type of surfactant was identified?
Based on the analysis of amino acids and FT-IR spectra, the product was identified as a cyclic lipopeptide, specifically a type of surfactin.
What was the outcome regarding fermentation timing?
The study observed an ideal fermentation time of 40 hours, after which the culture reached its stationary phase and achieved a maximum yield of 253.79 mg/L.
- Citar trabajo
- Unnati Patel (Autor), 2019, Biosynthesis and Development of Novel Method for Commercial Production of Biosurfactant Utilizing Waste Potato Peels, Múnich, GRIN Verlag, https://www.grin.com/document/1417662
