This study investigates the presence of Salmonella spp. and Listeria spp. in broiler chicken meat and products in Menoufia governorate, Egypt. A total of 100 random samples were collected, including 50 fresh chicken cuts (breast and thigh) and 50 frozen breaded half-cooked products (chicken nuggets and chicken burgers). The samples were transported to the Food Hygiene and Control Research Lab, Faculty of Veterinary Medicine, University of Sadat City, and analyzed following ISO 6579:2020 for Salmonella spp. and ISO 11290:2017 for Listeria spp.
The results revealed Salmonella contamination in 6% of the samples and Listeria contamination in 14%. Among the Salmonella isolates, three serotypes were identified: Salmonella paratyphi A (1%), Salmonella Heidelberg (1%), and Salmonella Typhimurium (4%). Real-time PCR confirmed the presence of the invA gene in all Salmonella-positive samples. For Listeria spp., six species were detected: L. monocytogenes (3%), L. ivanovii (2%), L. seeligeri (2%), L. innocua (3%), L. welshimeri (2%), and L. grayi (2%).
This study highlights the significant presence of foodborne pathogens in chicken meat and products, underscoring the need for stringent hygiene practices and regular monitoring to ensure public health safety.
Inhaltsverzeichnis (Table of Contents)
- Abstract
- Dedication
- Acknowledgment
- Chapter 1: Introduction
- Chapter 2: Materials and Methods
- Chapter 3: Results
- Chapter 4: Discussion
- Chapter 5: Conclusion
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This thesis aims to determine the prevalence of Salmonella and Listeria species in poultry meat and products in Menoufia governorate, Egypt, and to characterize the isolates molecularly. The study contributes to understanding the microbiological safety of poultry products in the region.
- Prevalence of Salmonella and Listeria in poultry meat and products.
- Molecular characterization of isolated Salmonella serotypes.
- Species identification of isolated Listeria.
- Assessment of microbiological safety of poultry products.
- Public health implications of findings.
Zusammenfassung der Kapitel (Chapter Summaries)
Chapter 1: Introduction: This chapter provides background information on Salmonella and Listeria, their significance as foodborne pathogens, and the importance of monitoring their prevalence in poultry products. It sets the stage for the study by highlighting the public health concerns related to contaminated poultry and the need for effective control measures. The introduction likely discusses the prevalence of these pathogens globally and specifically in Egypt, laying the groundwork for the research presented in the thesis.
Chapter 2: Materials and Methods: This chapter details the methodology employed in the study, including the sampling strategy, sample collection and processing procedures, microbiological analysis techniques (ISO 6579:2020 for Salmonella and ISO 11290:2017 for Listeria), and molecular characterization methods (serotyping and R-T PCR for invA gene detection). This section provides a thorough description of the experimental design and procedures, allowing for replication of the study and evaluation of the methods' validity. The methods used for isolation, identification, and typing of the bacterial species are thoroughly described, establishing the scientific rigor of the research.
Chapter 3: Results: This chapter presents the findings of the study, including the prevalence rates of Salmonella and Listeria in the sampled poultry meat and products. Specific serotypes of Salmonella identified and the species distribution of Listeria are detailed. The results section likely includes statistical analysis of the data, demonstrating the significance of the findings. The prevalence data of both pathogens is presented, potentially broken down by product type (fresh vs. frozen) and specific pathogens.
Chapter 4: Discussion: This chapter interprets the results in the context of existing literature and discusses their implications for public health. The findings are compared to other studies on poultry contamination, potential sources of contamination are explored, and the effectiveness of current food safety practices are evaluated. This section likely explains the significance of the detected pathogens, discusses the limitations of the study, and offers recommendations for future research and improved food safety strategies. This chapter synthesizes the findings, making connections between the prevalence of specific pathogens, and identifies areas for future research and/or intervention.
Schlüsselwörter (Keywords)
Salmonella spp., Listeria spp., Listeria monocytogenes, poultry meat, food safety, R-T PCR, invA gene, serotyping, foodborne pathogens, Egypt, Menoufia governorate, microbiological analysis.
Häufig gestellte Fragen
What is the content of this document?
This document is a language preview from a publishing company, including a title, table of contents, objectives and key themes, chapter summaries, and keywords related to a thesis or research paper.
What topics does the document cover?
The document primarily covers the prevalence of Salmonella and Listeria species in poultry meat and products, specifically in the Menoufia governorate of Egypt. It also delves into the molecular characterization of the isolates.
What is included in the table of contents?
The table of contents includes the following sections: Abstract, Dedication, Acknowledgment, Chapter 1: Introduction, Chapter 2: Materials and Methods, Chapter 3: Results, Chapter 4: Discussion, and Chapter 5: Conclusion.
What are the main objectives and key themes of the study described?
The main objectives are to determine the prevalence of Salmonella and Listeria in poultry, characterize the isolates molecularly, assess the microbiological safety of poultry products, and understand the public health implications of the findings. The key themes include prevalence, molecular characterization, species identification, microbiological safety assessment, and public health implications.
Can you summarize each chapter?
Chapter 1: Introduction: Provides background information on Salmonella and Listeria, their significance as foodborne pathogens, and the importance of monitoring their prevalence in poultry.
Chapter 2: Materials and Methods: Details the study's methodology, including sampling strategy, sample processing, microbiological analysis techniques (ISO 6579:2020 for Salmonella and ISO 11290:2017 for Listeria), and molecular characterization methods.
Chapter 3: Results: Presents the study's findings, including the prevalence rates of Salmonella and Listeria and the identification of specific serotypes.
Chapter 4: Discussion: Interprets the results in the context of existing literature and discusses their implications for public health, potential contamination sources, and the effectiveness of current food safety practices.
What keywords are associated with this document?
The keywords include Salmonella spp., Listeria spp., Listeria monocytogenes, poultry meat, food safety, R-T PCR, invA gene, serotyping, foodborne pathogens, Egypt, Menoufia governorate, and microbiological analysis.
What are the microbiological analysis techniques used?
The microbiological analysis techniques used include those outlined in ISO 6579:2020 for Salmonella and ISO 11290:2017 for Listeria.
What is the significance of the invA gene in this study?
The invA gene is likely used as a target for the detection of Salmonella via R-T PCR (real-time polymerase chain reaction) in the molecular characterization process.
Where was the study conducted?
The study was conducted in the Menoufia governorate of Egypt.
- Quote paper
- Gehad A. Ibrahim (Author), 2023, Prevalence and Molecular Characterization of Salmonella and Listeria Species in Poultry Meat and their Products, Munich, GRIN Verlag, https://www.grin.com/document/1524103
