Nanotechnology in present era gaining value due to the capability of modulating metals into their nanoparticles due to this our work reports on the green synthesis of metal nanoparticles which has proved advantageous over chemical synthetic methods due to its low cost and environmental compatibility. The Phytochemicals present in the leaf extract act as capping and stabilizing agents for AgNPs that can rapidly reduce silver ions (Ag+ to Ag) and resulted in a color change indicating the formation of silver nanoparticles. Synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy (FESEM), and Fourier Transform Infra-red Spectroscopy (FTIR) techniques.
The UV-Vis spectra showed a peak at 459nm. FESEM revealed that the synthesized silver nanoparticles are spherical and circular in morphology and the overall size is between 36-45nm. FTIR spectroscopy analysis showed the presence of alkaloid, polyphenols and amide groups likely to be responsible for the green synthesis of silver nanoparticles. The synthesized nanoparticles showed efficient antibacterial as well as antioxidant activity. The effect of the increase of metal salt solution was also studied. The study revealed the formation of dendrites with the increase in metal salt concentration. This study focuses on a cost-efficient, eco-friendly and safe technique for the synthesis of silver nanoparticle using leaves of medicinal plants.
Table of Contents
1.0 Introduction
2. Silver Nanoparticles
2.0 Materials and Methods
2.1. Materials
2.2 Methodology
3.0 Result and Discussion
3.1. Phytochemical analysis of A. oleracea Leaf Extract
3.2. Characterization of AgNPs by UV-Vis spectroscopy
3.3. Fourier transforms infrared spectroscopy (FT-IR) analysis
3.4. Field Emission Scanning Electron Microscope (FESEM) analysis
3.5. Antibacterial Assays
4.0 Conclusion
Research Objectives and Themes
This study aims to develop a cost-efficient, eco-friendly, and safe method for the green synthesis of silver nanoparticles (AgNPs) utilizing the leaf extract of the medicinal plant Acmella oleracea, while evaluating their morphological characteristics and antibacterial efficacy.
- Green synthesis of silver nanoparticles using plant-mediated biological routes
- Phytochemical analysis of Acmella oleracea leaf extracts
- Material characterization via UV-Vis, FTIR, and FESEM techniques
- Assessment of antibacterial and antioxidant activities
- Optimization of nanoparticle stability and morphological control
Excerpt from the Book
3.1. Phytochemical analysis of A. oleracea Leaf Extract
Dried leaves of A. oleracea were analyzed for its chemical content, and this revealed the presence of active constituents i.e. alkaloids, sterols, triterpene, amino acid and phenolic compounds have been done with the chemical tests.
The various extract/ fractions were basified with ammonia and extracted with chloroform. The chloroform solution was acidified with dilute hydrochloric acid. The acid layer was used for testing the alkaloids [48].
Hager’s test: To 2ml of each extract, a few drops of Hager’s (saturated picric acid solution) reagent were added. Formation of a bright yellow colored precipitate indicates the presence of alkaloids [49].
Summary of Chapters
1.0 Introduction: Provides an overview of nanotechnology, defining nanoparticles and contrasting chemical synthesis methods with sustainable, plant-mediated green synthesis approaches.
2. Silver Nanoparticles: Discusses the unique properties of AgNPs, their industrial and medical applications, and reviews previous research on green synthesis using various plant extracts.
2.0 Materials and Methods: Details the collection of Acmella oleracea leaves and outlines the experimental protocols for preparing plant extracts and synthesizing silver nanoparticles.
2.1. Materials: Lists the specific source, location of plant collection, and technical-grade chemicals utilized in the synthesis process.
2.2 Methodology: Describes the specific reduction process, centrifugation, and purification steps taken to yield stable AgNPs.
3.0 Result and Discussion: Analyzes the experimental data, presenting findings on the synthesis success and chemical composition of the obtained particles.
3.1. Phytochemical analysis of A. oleracea Leaf Extract: Reports the presence of active secondary metabolites in the leaf extract responsible for the reduction of silver ions.
3.2. Characterization of AgNPs by UV-Vis spectroscopy: Explains the confirmation of AgNP formation through surface plasmon resonance peaks observed at 459 nm.
3.3. Fourier transforms infrared spectroscopy (FT-IR) analysis: Identifies the functional groups present on the nanoparticle surface that facilitate stabilization and reduction.
3.4. Field Emission Scanning Electron Microscope (FESEM) analysis: Details the morphological findings, confirming that the synthesized particles are spherical and within the size range of 36-45 nm.
3.5. Antibacterial Assays: Presents the efficacy results of the AgNPs against E. coli compared to standard antibiotics.
4.0 Conclusion: Sums up the viability of A. oleracea as an efficient, eco-friendly resource for future large-scale medical and industrial nanoparticle applications.
Keywords
Silver nanoparticles, Characterization techniques, Antibacterial activity, Antioxidant activity, Phytochemical Properties, Green synthesis, Acmella oleracea, Nanotechnology, UV-Vis spectroscopy, FTIR analysis, FESEM, Biological synthesis, Sustainable chemistry
Frequently Asked Questions
What is the primary focus of this research?
The research focuses on the green synthesis of silver nanoparticles using the leaf extract of Acmella oleracea as an environmentally friendly alternative to conventional chemical synthesis.
What are the central thematic areas?
The study covers nanotechnology, plant-mediated biosynthesis, analytical characterization, and the assessment of antibacterial/antioxidant properties.
What is the main research objective?
The objective is to establish a safe, cost-effective, and reproducible biological method for synthesizing stable silver nanoparticles using medicinal plant resources.
Which scientific methods are utilized?
The paper employs UV-Vis spectroscopy, Fourier Transform Infra-red (FTIR) spectroscopy, and Field Emission Scanning Electron Microscopy (FESEM) for characterization, alongside disc diffusion methods for antibacterial testing.
What does the main part of the work cover?
The main part encompasses the extraction methodology, chemical testing of the phytochemical content, and systematic analysis of nanoparticle morphology and antimicrobial efficacy.
What are the key descriptive terms for this work?
Key terms include green synthesis, silver nanoparticles, Acmella oleracea, and biomedical efficacy.
How does Acmella oleracea contribute to the synthesis process?
The leaf extract of Acmella oleracea acts as a natural reducing and capping agent due to its high content of secondary metabolites, which facilitates the reduction of Ag+ ions into stable silver nanoparticles.
What were the morphological findings of the synthesized nanoparticles?
FESEM analysis revealed that the synthesized particles were spherical, smooth-surfaced, and possessed an average size range between 36 and 45 nm.
How did the AgNPs perform against bacteria?
The synthesized AgNPs exhibited efficient antibacterial activity, showing stronger inhibitory effects against tested pathogens compared to standard conventional antibiotics.
- Arbeit zitieren
- Asheesh Sharma (Autor:in), 2025, Synthesis, Characterization and Antibacterial Activity of AgNPs by Using Leaf Extract of Acmella Oleracea, München, GRIN Verlag, https://www.grin.com/document/1696177
