Flow Cytometry of Intracellular Calcium in Platelets


Textbook, 2012

30 Pages


Excerpt


Contents

Introduction
The Role of Calcium in Intracellular Signal Transduction
Sources of Calcium
Signal Transduction
Activation of Platelets
Adhesion
Shape Change
Aggregation
Secretion
Procoagulant Activity
Soluble Platelet Agonists
Inhibition of Platelets by cAMP

Flow Cytometry
Principle
Parameters
Monoclonal Antibodies
Isotype Control
Compensation
Monoclonal Antibodies Used for the Flow Cytometric Characterization of Platelets
Intracellular Probes
Presentation and Analysis of Data
Dotplots and Identification of Cells
Further Analysis
Regions and Gates

The Use of Fluo-3 as Intracellular Calcium Indicator
Brief Introduction
Fluo-3

Application
Flow Cytometric Analysis

Conclusion

References

Abbreviations

Index

Excerpt out of 30 pages

Details

Title
Flow Cytometry of Intracellular Calcium in Platelets
Author
Year
2012
Pages
30
Catalog Number
V190163
ISBN (eBook)
9783656146520
ISBN (Book)
9783656146629
File size
711 KB
Language
English
Keywords
flow, cytometry, intracellular, calcium, platelets
Quote paper
Thomas Gamsjäger (Author), 2012, Flow Cytometry of Intracellular Calcium in Platelets, Munich, GRIN Verlag, https://www.grin.com/document/190163

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