Excerpt
Contents
Introduction
The Role of Calcium in Intracellular Signal Transduction
Sources of Calcium
Signal Transduction
Activation of Platelets
Adhesion
Shape Change
Aggregation
Secretion
Procoagulant Activity
Soluble Platelet Agonists
Inhibition of Platelets by cAMP
Flow Cytometry
Principle
Parameters
Monoclonal Antibodies
Isotype Control
Compensation
Monoclonal Antibodies Used for the Flow Cytometric Characterization of Platelets
Intracellular Probes
Presentation and Analysis of Data
Dotplots and Identification of Cells
Further Analysis
Regions and Gates
The Use of Fluo-3 as Intracellular Calcium Indicator
Brief Introduction
Fluo-3
Application
Flow Cytometric Analysis
Conclusion
References
Abbreviations
Index
- Quote paper
- Thomas Gamsjäger (Author), 2012, Flow Cytometry of Intracellular Calcium in Platelets, Munich, GRIN Verlag, https://www.grin.com/document/190163
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