The leaves of Crateva religiosa are used in traditional medicine for the treatment of various kinds of wounds. The present study was undertaken to evaluate the effect of herbal ointments formulated with the methanol extract of Crateva religiosa leaf on experimentally induced wounds in rats using the excision wound model. The animals were divided into five groups of five animals each. In the test groups 10, 15 and 20% extract of C. religiosa were topically applied once a day as ointment on groups 2, 3 and 4, respectively. Group 1 received blank petroleum jelly topically once a day while group 5 was treated topically with the standard drug, penicillin ointment. The mean wound sizes of all the animals were measured on the 3rd, 9th, 15th and 18th day post wounding and the mean percentage reduction in wound area calculated. Results obtained showed a dose dependent percentage reduction in wound area with the the 15% C. religiosa leaf extract (group 3) recording the highest mean percentage wound reduction (83 %) compared to the standard drug penicillin ointment which achieved complete wound healing after 18 days post wounding. This study suggests that C. religiosa leaf extract could be developed as a therapeutic agent for wound healing.
Inhaltsverzeichnis (Table of Contents)
- Abstract
- Introduction
- Materials and Methods
- Plant Material
- Extraction
- Chemicals and Drugs
- Experimental Animals
- Preparation of herbal ointment
- Wound induction
- Measurement of Wound area
- Results and Discussion
- References
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
The main objective of this study was to evaluate the wound-healing efficacy of a herbal ointment formulated with Crateva religiosa leaf extract in an experimental rat model. The study aimed to determine if the extract could accelerate wound healing and to assess the dose-dependent effect of the ointment.
- Wound healing properties of Crateva religiosa leaf extract.
- Dose-response relationship between Crateva religiosa extract concentration and wound healing.
- Comparison of the herbal ointment's efficacy to a standard wound healing agent (penicillin ointment).
- Evaluation of the wound healing process using an excision wound model in rats.
- Traditional uses of Crateva religiosa and the scientific validation of its efficacy.
Zusammenfassung der Kapitel (Chapter Summaries)
Abstract: This study investigated the wound-healing effect of a herbal ointment made from Crateva religiosa leaf extract on rats with experimentally induced wounds. Using an excision wound model, rats were treated with different concentrations (10%, 15%, and 20%) of the extract. The 15% concentration showed the best results, achieving 83% wound reduction, comparable to the 100% healing achieved by penicillin ointment after 18 days. The study suggests the potential of C. religiosa leaf extract as a wound-healing agent.
Introduction: This section provides background information on wound healing, classifying it into three types and highlighting the ongoing research for effective wound healing agents. It introduces Crateva religiosa, a plant traditionally used for wound healing, and explains the study's purpose: to scientifically investigate the wound healing effects of its methanolic leaf extract.
Materials and Methods: This chapter details the research methodology. It describes the collection and preparation of Crateva religiosa leaves, the extraction process using methanol, and the preparation of the herbal ointment with different concentrations of the extract. It further outlines the experimental design, including the use of albino rats, the induction of excision wounds, and the measurement of wound area at various time points. The statistical analysis method employed is also explained.
Results and Discussion: This chapter presents the findings of the study. It reveals a dose-dependent relationship between the concentration of Crateva religiosa extract and wound healing. The ointment with 15% extract showed the most significant wound reduction (83%), comparable to the penicillin ointment control. The discussion analyzes the implications of these findings, highlighting the potential of C. religiosa as a cost-effective and seemingly non-toxic wound healing agent. The results are presented in table and figure formats showing the percentage of wound healing over time for each group.
Schlüsselwörter (Keywords)
Crateva religiosa, wound healing, herbal ointment, methanol extract, excision wound model, rats, dose-dependent, penicillin ointment, traditional medicine, wound contraction, therapeutic agent.
Frequently Asked Questions: Wound Healing Efficacy of Crateva religiosa Leaf Extract
What is the main objective of this study?
The study's primary goal was to evaluate the effectiveness of a herbal ointment made from Crateva religiosa leaf extract in promoting wound healing in rats. It aimed to determine if the extract could accelerate healing and assess how the ointment's effectiveness changed with different concentrations.
What are the key themes explored in this research?
The research explores the wound-healing properties of Crateva religiosa leaf extract, the relationship between the extract's concentration and wound healing, a comparison of the herbal ointment's efficacy to penicillin ointment, and the evaluation of the wound healing process using an excision wound model in rats. It also touches upon the traditional uses of Crateva religiosa and provides scientific validation of its potential efficacy.
What were the methods used in this study?
The study involved preparing a herbal ointment with varying concentrations (10%, 15%, and 20%) of Crateva religiosa leaf extract obtained through methanol extraction. An excision wound model was used in albino rats, and the wound area was measured at regular intervals. Penicillin ointment served as a control. Statistical analysis was used to interpret the results.
What are the key findings of the study?
The study found a dose-dependent effect, with the 15% concentration of the Crateva religiosa extract ointment showing the most significant wound reduction (83%). This result was comparable to the 100% healing achieved by the penicillin ointment after 18 days. The results suggest the potential of C. religiosa leaf extract as a cost-effective and seemingly non-toxic wound healing agent.
What are the chapter summaries?
The study includes an abstract summarizing the findings; an introduction providing background on wound healing and Crateva religiosa; a materials and methods section detailing the experimental design and procedures; a results and discussion section presenting and interpreting the data; and finally, a reference section citing the sources used.
What are the key words associated with this study?
Key words include Crateva religiosa, wound healing, herbal ointment, methanol extract, excision wound model, rats, dose-dependent, penicillin ointment, traditional medicine, wound contraction, and therapeutic agent.
What is the overall conclusion of the study?
The study concludes that Crateva religiosa leaf extract shows promise as a potential wound-healing agent, demonstrating comparable efficacy to penicillin ointment in an experimental rat model. Further research is likely needed to confirm these findings in human clinical trials.
- Quote paper
- Kingsley Adimabua (Author), G. A. Awemu (Author), C. A. Okonkwo (Author), A. M. Odiegwu (Author), 2013, Evaluation of the Wound Healing Effect of Herbal Ointment Formulated with "Crateva Religiosa" Leaf Extract, Munich, GRIN Verlag, https://www.grin.com/document/207816
