Extraction of Pure Ketamine Powder and Study their Analgesic Effect as a Gel on Mice Using a Hot – Plate Test

Wissenschaftlicher Aufsatz , 2012 , 12 Seiten , Note: 3

Chemie - Sonstiges

The present study was undertaken to extract ketamine powder from ketamine hydrochloride by precipitate ketamine. After that we examine the purity of this powder by infra-red (FTIR) and ultra-violet(UV) spectroscopy. ketamine gel in different concentrations was prepared ( 0.5 , 1 , 5 , 10 , 15 )% to evaluate the antinociceptive activity. ketamine powder was seen is pure and this show in infra-red and ultra-violet scanner. Ketamine gel at concentrations 0.5, 1, 5, 10,15) % produce antinociceptive in mice (5.6±2.2) (4.4±2.0) (8.2±4.3) (10.6±5.2) (8±2.1) second after 2 min respectively by using a hot plate test in comparison with control(2.4±2). The percentage of maximum possible effect (MPE) increased from (9.9) % in control group to (23.3) (18.3) (34.2) (44.2) (33.3)% respectively according to the concentrations of ketamine gel after 2 min . Purification of ketamine powder from ketamine solution and use as a gel to could be of value relief pain by topical application.

Table of Contents

• Introduction
• Materials and Methods
  • Preparation of ketamine powder
  • Infrared Spectroscopy.
  • Electronic Spectra Measurement:
  • Preparation of ketamine gel
  • Determination of Analgesic Activity of Ketamine Gel by Using a Hot -Plate Test:
  • Statistical analysis
• Results
• Discussion

Objectives and Key Themes

This research aims to extract pure ketamine powder from ketamine hydrochloride solution and prepare a ketamine gel. The study then investigates the analgesic effects of topically applied ketamine gel on mice using the hot plate test.

• Extraction and purification of ketamine powder
• Preparation of ketamine gel for topical application
• Evaluation of analgesic activity of ketamine gel in mice
• Investigation of the role of NMDA receptors in the analgesic effects of ketamine
• Comparison of the analgesic efficacy of ketamine gel with various concentrations

Chapter Summaries

The study begins by outlining the historical use of ketamine as a dissociative anesthetic and its analgesic properties. The authors then detail the methods employed to extract pure ketamine powder from ketamine hydrochloride solution and prepare ketamine gel in various concentrations.

The results section presents the analysis of the extracted ketamine powder using infrared and ultraviolet spectroscopy, confirming its purity. Analgesic effects of the prepared ketamine gel at different concentrations are evaluated using the hot plate test in mice.

Keywords

This study focuses on the extraction, purification, and topical application of ketamine. Key terms and concepts include ketamine, ketamine hydrochloride, ketamine gel, analgesia, NMDA receptors, hot-plate test, infrared spectroscopy, ultraviolet spectroscopy, and topical drug delivery.

• Hochschule: University of Mosul
• Veranstaltung: Dental Chemistry
• Note: 3
• Autoren: Amer Taqa (Autor:in), Banan N. Alhussary (Autor:in), Ghada A. Taqa (Autor:in)
• Erscheinungsjahr: 2012
• Seiten: 12
• Katalognummer: V209705
• ISBN (eBook): 9783656375425
• ISBN (Buch): 9783656382805
• Sprache: Englisch
• Schlagworte: extraction, pure, ketamine, powder, study, analgesic, effect, mice, using, plate, test