ANTIULCER SCREENING OF SELECTED PLANTS OF PLUMBAGO SPECIES & THE EFFECT OF PLUMBAGIN IN GASTRIC CELL LINES
Objectives: The objective of the study is to evaluate the antiulcer effect of the Plumbago species and to investigate the most effective plant among the selected Plumbaginales, P.auriculata, P.indica & P.zeylanica after the nvitro evaluation.
Materials and Methods: MTT assay was used to perform the cytotoxic effect of the Plumbagin and to scrutinize the least cytotoxic among the Plumbaginales for gastroprotective effect. The DPPH scavenging assay, Lipid peroxidase inhibition assay, Acid neutralising capacity test and Ethanol induced ulcer model were used for assessing the antiulcer effective plant for invivo studies. Ranitidine (20mg/kg, p.o) was used as reference drug in the study.
Results and Discussion: MTT results revealed that the Plumbagin showed cytotoxic activity at 40.18μg/ml, P.auriculata is having least cytotoxic effect. Even though significant antiulcer activity were exhibited by all the Plumbaginales and Plumbagin showed significant antioxidant and acid neutralising assays, P.auriculata had decreased the number of ulcers and increased in the percentage gastric protection.
Conclusion: P.auriculata showed antiulcer study and the results were compared by Plumbagin, P.indica and P.zeylanica. A future prospect on mechanism based studies and isolation of the constituents is needed for the individual plants.
Table of Contents
1. Introduction
2. Literature review
2.1.1. Normal anatomy of the stomach
2.1.2. Histology
2.2.1. Gastric ulcer and peptic ulcer disease
2.2.2. Pathophysiology
2.2.3. The regulation of acid secretion by parietal cells
2.2.4. Alteration of mucosal defense and healing
2.2.5. Abnormal gastric motility
2.3. Etiology-specific pathophysiology
2.3.1. Helicobacter pylori
2.3.2. Nonsteroidal anti-inflammatory drugs
2.3.3. Zollinger-Ellison's syndrome and other hypersecretory conditions
2.3.4. Stress-related erosive syndrome
2.3.5. Gastroesophageal reflux disease
2.3.6. Signs and symptoms
2.4. Treatment
2.5. Gastric cytoprotective effects
2.6. Animal models used in the screening of anti ulcer activity
2.6.1. Aspirin induced ulcers
2.6.2. Ethanol induced ulcers
2.6.3. Pylorus ligation induced ulcers
2.6.4. Water immersion stress induced model
2.6.5. Indomethacin induced ulcers
2.6.6. Histamine induced ulcers
2.6.7. Reserpine induced ulcers
2.6.8. Serotonin induced ulcers
2.6.9. Acetic acid induced ulcers
2.6.10. Hydrochloric acid induced ulcers
2.7. Role of antioxidant studies in antiulcer screening
2.7.1. Free radical and radical reaction
2.7.2. Antioxidant defense
2.7.3. Mode of action antioxidants
2.7.4. Concept of oxidative stress
2.7.5. Molecular damage induced by free radicals
2.7.5.1. Lipid peroxidation
2.7.5.2. Dpph free radical scavenging assay
2.7.6. Oxidative stress in gastric mucosa
2.7.7. Endogenous gastroprotective molecules
2.7.8. Oxidative stress in the stomach and acute ethanol toxicity
2.7.9. Gastric ulcers and erosions caused by ethanol
2.8. Antiulcer properties of specific phytochemicals
2.8.1. Herbal medicines and natural compounds used in treatment of ulcer
2.8.2. Alkaloids
2.8.3. Flavonoids
2.8.4. Saponins
2.8.5. Tannins
3. Plant profile
3. 1. Plumbago species
3.1.1. Classification
3.1.2. India
3.1.3. Western Europe
3.1.4. Traditional South African uses
3.1.5. European medicinal uses
3.2. Plumbago auriculata
3.2.1. Synonyms
3.2.2. Vernacular names
3.2.3. General information
3.2.4. Traditional uses
3.2.5. Pre-clinical data- pharmacology
3.2.6. Toxicities
3.3. Plumbago indica
3.3.1. Synonyms
3.3.2. Vernacular names
3.3.3. General information
3.3.4. Traditional uses
3.3.5. Pre-clinical data- pharmacology
3.3.6. Toxicities
3.4. Plumbago zeylanica
3.4.1. Synonyms
3.4.2. Vernacular names
3.4.3. General information
3.4.4. Traditional uses
3.4.5. Pre-clinical data- pharmacology
3.4.6. Toxicities
4. Hypothesis, aim & objective
5. Plan of work
6. Materials & methods
6.1. Materials
6.2. Extraction
6.2.1. Herbal plant collection
6.2.2. Plumbgin extraction
6.2.3. Preparation of plumbagin free alcoholic extract
6.2.4.Peliminary phytochemical analysis
6.2.5.Estimation of the amount of plumbagin in the extracts
6.3.In-vitro methods:-
6.3.1. Micro culture tetrazolium(mtt) assay
6.3.2. DPPH assay
6.3.3. Lipid peroxidase assay
6.3.4. Acid nuetralising capacity
6.3.5. Ealuation of ethanol induced antiulcer activity
6.3.6. Statistical analysis
6.4. In-vivo methods
6.4.1.Aspirin induced model
6.4.2. Ethanol induced model
6.4.3. Histological studies
6.4.5. Statistical analysis
7. Results
7.1. Extraction
7.1.1. Herbal plant collection
7.1.2. Plumbagin extraction
7.1.3. Preparation of Plumbagin free alcoholic extract
7.1.4.Preliminary phytochemical analysis
7.1.5.Estimation of the amount of Plumbagin in the extracts
7.2. In-vitro methods:-
7.2.1. Micro culture tetrazolium(mtt) assay
7.2.2. DPPH assay
7.2.3. Lipid peroxidase assay
7.2.4. Acid nuetralising capacity
7.2.5. Evaluation of ethanol induced antiulcer activity
7.2.6.Statistical analysis
7.3. In-vivo methods
7.3.1.aspirin induced model
7.3.2. Ethanol induced model
7.3.3. Histological studies
7.3.4. Statistical analysis
8. Discussion
9. Conclusion
10. References
Objectives & Key Topics
The dissertation aims to evaluate the anti-ulcer efficacy of selected Plumbago species—P. auriculata, P. indica, and P. zeylanica—and to investigate the effects of Plumbagin on gastric cell lines. The study seeks to determine the most effective plant candidate for therapeutic use by analyzing their phytochemical composition, antioxidant properties, and their impact on induced gastric ulcer models.
- Phytochemical screening of various Plumbago species extracts.
- Evaluation of cytotoxic activities on HGE-17 gastric epithelial cell lines.
- Antioxidant assessment using DPPH and lipid peroxidase assays.
- In-vitro and in-vivo anti-ulcer screening using aspirin and ethanol-induced models.
- Statistical validation of gastroprotective efficacy and comparative analysis of different extracts.
Excerpt from the Book
1. INTRODUCTION
Peptic ulcer disease (PUD) is one of the most common, chronic gastrointestinal disorder in modern era. It has become a common global health problem affecting a large number of people worldwide and also still a major cause of morbidity and mortality. An estimated 15,000 deaths occur each year as a consequence of PUD. A report of the Indian Council of Medical Research on the epidemiology of peptic ulcer in India showed that the overall incidence of the disease ranged from 1 to 6.5 per thousand in the age group of 15 years and above in the selected urban population.
Ulcer is an open sore that develops on the inside lining of the stomach (a gastric ulcer) or the small intestine (a duodenal ulcer). Both types of ulcers are also referred to as PUD and can be characterized by inflamed lesions or excavations of the mucosa and tissue that protect the gastrointestinal tract. The most common symptom of a peptic ulcer is a burning or gnawing pain in the center of the abdomen (stomach). It was a belief that lifestyle factors, such as diet, smoking, alcohol and stress were the main causes of peptic ulcers. While these factors may play a limited role, it is known that the leading cause of peptic ulcers is a type of bacteria called Helicobacter pylori (H. pylori) can infect the stomach and small intestine; and in some people, the bacteria can irritate the inner layer of the stomach and small intestine, leading to the formation of an ulcer. Painkillers known as nonsteroidal anti-inflammatory drugs (NSAIDs), which include aspirin and ibuprofen, are the second most common cause of peptic ulcers that can irritate the lining of the stomach and small intestine in some people, particularly if they are taken on a long-term basis.
Summary of Chapters
1. Introduction: Outlines the prevalence and global burden of Peptic Ulcer Disease (PUD), the limitations of current allopathic treatments, and the rationale for ethnopharmacological research into traditional plant-based alternatives.
2. Literature review: Provides a comprehensive overview of gastric anatomy, histology, and the pathophysiology of peptic ulcers, along with an examination of antioxidant mechanisms and current scientific knowledge regarding Plumbago species.
3. Plant profile: Details the botanical classification, distribution, traditional medicinal uses, and pre-clinical pharmacological data for the three studied species: P. auriculata, P. indica, and P. zeylanica.
4. Hypothesis, aim & objective: States the primary research hypothesis regarding the anti-ulcer potential of the selected plants and defines the specific goals of the experimental study.
5. Plan of work: Outlines the systematic procedural flow, including plant collection, extraction methods, in-vitro assays, and in-vivo models.
6. Materials & methods: Lists the chemical reagents, laboratory equipment, cell lines, and specific protocols used for phytochemical analysis, antioxidant testing, and anti-ulcer models.
7. Results: Presents the primary experimental data, including phytochemical yield, cytotoxicity assays, antioxidant efficacy, and the outcomes of the aspirin and ethanol-induced ulcer models, supported by statistical analysis.
8. Discussion: Interprets the experimental results, correlates findings with existing literature, and explores the role of phytochemicals like flavonoids and tannins in gastroprotection.
9. Conclusion: Summarizes the study's findings, highlighting P. auriculata as a potent candidate for anti-ulcer therapy, and suggests future research directions.
10. References: Provides the comprehensive bibliography used throughout the research.
Keywords
Plumbago, Peptic Ulcer Disease, Gastroprotection, Antioxidant, Plumbagin, Ethnopharmacology, Gastric mucosa, HGE-17 cell line, Aspirin-induced ulcer, Ethanol-induced ulcer, Flavonoids, Tannins, Phytochemical screening, Lipid peroxidation, DPPH assay.
Frequently Asked Questions
What is the primary focus of this research study?
The study focuses on the anti-ulcer screening of three specific Plumbago species (P. auriculata, P. indica, and P. zeylanica) and the evaluation of the biological effects of the compound Plumbagin in gastric cell lines.
What are the central themes covered in this dissertation?
The work integrates ethnobotanical traditional knowledge with modern pharmacological practices, focusing on gastric physiology, oxidative stress in the stomach, and the screening of herbal extracts for gastroprotective and antioxidant activity.
What is the main objective of the researcher?
The goal is to identify effective, natural anti-ulcer agents by systematically evaluating these plants, assessing their cytotoxicity, and confirming their protective effect against induced gastric damage in rats.
Which scientific methodology is utilized in the study?
The research uses a multi-faceted methodology, including phytochemical screening, in-vitro assays (MTT assay, DPPH assay, Lipid peroxidation assay, and acid-neutralizing capacity testing), and in-vivo models utilizing aspirin and ethanol-induced ulcers.
What subjects are discussed in the main body?
The main body examines the anatomy and histology of the stomach, the underlying causes of peptic ulcers (like H. pylori and NSAIDs), the concept of oxidative stress, and detailed pharmacological profiles of the studied plants.
Which keywords characterize this research?
Key terms include Plumbago, gastroprotection, antioxidant, PUD, and ethnopharmacology, reflecting the medicinal and botanical nature of the project.
Why was the extraction method for Plumbagin-free alcoholic extract important?
Because Plumbagin has a dual nature—showing both medicinal and toxic effects—the researchers needed to prepare extracts that were free of Plumbagin to assess whether the anti-ulcer activity was independent of its high cytotoxicity.
What did the results reveal about P. auriculata?
The experimental results demonstrated that P. auriculata exhibits the least cytotoxic effect compared to other species while showing significant antioxidant activity and strong gastroprotective potential in ethanol-induced ulcer models.
- Quote paper
- Dr.Sibi P.Ittiyavirah (Author), 2012, Antiulcer Screening of Selected Plants of Plumbago Species and the Effect of Plumbagin in Gastric Cell Lines, Munich, GRIN Verlag, https://www.grin.com/document/268500
