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Altered production of organic acid and pigments by microbes under influence of microwave radiation

Microwave Mutagenesis

Title: Altered production of organic acid and pigments by microbes under influence of microwave radiation

Scientific Study , 2014 , 75 Pages , Grade: A

Autor:in: Assistant Professor Vijay Kothari (Author), Haren Gosai (Author), Shreya Raval (Author), Vimla Chaudhary (Author)

Biology - Micro- and Molecular Biology
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Summary Excerpt Details

This study attempted at microwave mutagenesis of: (i) Lactobacillus plantarum for lactic acid overproduction, and (ii) Streptococcus mutans for reduced lactic acid production. Lactic acid is among the microbiological products with high market potential. Lactic acid is also an important virulence factor in formation of dental caries by S. mutans, as the acid produced by the bacteria leads to demineralisation of the teeth. Two of the mutants obtained (one from each organism) were able to maintain the altered lactic acid production till 10 generations. However the magnitude of alteration in lactic acid producing ability of the mutants went on decreasing over generations. The microwave effects observed in this study seem largely to be athermal in nature. Investigation of the mutants obtained at molecular level may result in identification of novel mutations responsible for altered lactic acid production. These mutations can then be introduced into a suitable organism either for better industrial production of lactic acid, or for constructing new probiotic strain(s) for possible application in maintenance of oral health.

Effect of low power (90 W) microwave (2,450 MHz) radiation on bacterial growth and pigment production was studied in three different bacteria. Microwave exposure of 2-6 min duration was able to alter growth and pigment production (prodigiosin production by Serratia marcescens, violacein production by Chormobacterium violaceum, and staphyloxanthin production by Staphylococcus aureus) in the test organisms significantly. In this study, pigment production was estimated in the cell population originated from microwave treated inoculum, and not directly in the MW treated cells. Thus the alterations in pigment production and/or secretion might have been transferred from the originally MW treated cells to their daughter cells (who did not receive direct MW exposure), indicating the mutagenic influence of microwave radiation. Heavy prodigiosin overproduction observed in one of the test tubes inoculated with microwave treated S. marcescens could not be sustained by daughter populations corresponding to that tube, indicating the reversible nature of microwave induced mutation(s). The microwave effects observed in this study largely seem to be of athermal nature, as the thermal effect was minimized by use of ice during the microwave treatment.

Excerpt


Inhaltsverzeichnis (Table of Contents)

  • Prologue
    • Preamble

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This study focuses on investigating the non-thermal effects of microwave radiation on microbial cells. The research aims to explore the hypothesis that exposure to low-power microwaves can influence microbial growth, lactic acid production, and the production of pigments like prodigiosin, staphyloxanthin, and violacein.

  • Investigate the non-thermal effects of microwave radiation on microbial cells.
  • Determine the impact of microwave exposure on microbial growth rates.
  • Analyze the influence of microwave radiation on the production of lactic acid and pigments.
  • Explore the potential for microwave-induced genetic changes in microbial cells.
  • Examine the potential for microwave-induced changes to be passed on to subsequent generations of microbes.

Zusammenfassung der Kapitel (Chapter Summaries)

  • Prologue: This chapter provides an overview of the history of research on the interaction of electromagnetic fields (EMFs) with biological systems. It discusses the properties of microwaves, their applications, and the potential for both thermal and non-thermal effects on biological systems. The chapter also highlights the importance of studying the sub-lethal effects of microwave radiation on microbes.

Schlüsselwörter (Keywords)

Microwave radiation, non-thermal effects, microbial growth, lactic acid production, pigments, prodigiosin, staphyloxanthin, violacein, genetic changes, microbial mutagenesis.

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Details

Title
Altered production of organic acid and pigments by microbes under influence of microwave radiation
Subtitle
Microwave Mutagenesis
College
Nirma University  (Institute of Science)
Course
M.Sc.
Grade
A
Authors
Assistant Professor Vijay Kothari (Author), Haren Gosai (Author), Shreya Raval (Author), Vimla Chaudhary (Author)
Publication Year
2014
Pages
75
Catalog Number
V286832
ISBN (eBook)
9783656871187
ISBN (Book)
9783656871194
Language
English
Tags
altered microwave mutagenesis
Product Safety
GRIN Publishing GmbH
Quote paper
Assistant Professor Vijay Kothari (Author), Haren Gosai (Author), Shreya Raval (Author), Vimla Chaudhary (Author), 2014, Altered production of organic acid and pigments by microbes under influence of microwave radiation, Munich, GRIN Verlag, https://www.grin.com/document/286832
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