Optimization of Media Formulations for Callus Induction and direct Organogenesis in Justicia Gendrussa Burm Fil

The Uses and Advantages of an Exotic Medicinal Plant in Kerala

Wissenschaftliche Studie , 2017 , 39 Seiten , Note: 1.5

Biologie - Botanik

In this study, the callus initiation and rhizogenesis of Justicia gendarussa from nodal and leaf explants were established in Murashige and Skoog medium supplemented with different hormonal concentrations and also optimization of callus initiation at hormonal concentrations. As a result the callus was initiated on MS medium supplemented with different hormones. The higher concentration of NAA and BA induced callus from nodal and leaf explants. The higher concentrations of 2, 4 – D and kinetin induced callus on nodal explants. Lower concentration of 2, 4 – D and kinetin induce callus on leaf explants. The combination of NAA and kinetin induce rhizogenesis from nodal explants. The antimicrobial activity of callus was evaluated by well diffusion method. Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilus, Staphylococcus aureus, Klebsiella pneumonia were used as test organisms. The results showed that Justicia gendarussa have antimicrobial activity on Escherichia coli, Klebsiella pneumonia, and Staphylococcus aureus.

Keywords: Justicia gendarussa, callus initiation, direct organogenesis, antimicrobial assay.

Inhaltsverzeichnis (Table of Contents)

• Abstract
• 1. Introduction
  • 1.1 Aim
  • 1.2 Objectives
• 2. Review of literature
  • 2.1 Justicia gendarussa Burn Fil. Common names.
  • 2.2 Taxonomical classification: Justicia gendarussa Burn Fil.
• 3. Hypothesis
• 4. Materials and Methods
  • 4.1 Study area
  • 4.2 Plant material
  • 4.3 Equipment's used for experiment
  • 4.4 Culture media
    • 4.5 Compositions of MS media (Chawla H.S.)
    • 4.6 Preparation of MS media (stock preparation)
    • 4.7 Compositions of nutrient broth
    • 4.8 Preparation of nutrient broth
    • 4.9 Compositions of MHA
    • 4.10 Preparation of MHA
    • 4.11 Test organisms
    • 4.12 Experimental systems
    • 4.13 Preparation of MS media
    • 4.14 Preparation of explants
    • 4.15 Surface sterilization of explants
    • 4.16 Inoculation procedure
    • 4.17 Statistical analysis
• 5. Results and discussion
  • 5.1 Establishment of callus cultures from nodal and leaf explants.
  • 5.2 Establishment of direct organogenesis from nodal explants
  • 5.3 Optimization of callus initiation of Justicia gendarussa
  • 5.4 Evaluation of antimicrobial activity
• 6. Conclusions
• References

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This study aims to optimize media formulations for callus induction and direct organogenesis in Justicia gendarussa Burm Fil., an exotic medicinal plant found in Kerala. The research focuses on establishing effective protocols for in vitro propagation and exploring the potential of this plant for medicinal applications.

• Optimization of media formulations for callus induction and direct organogenesis in Justicia gendarussa.
• Establishment of in vitro propagation techniques for Justicia gendarussa.
• Investigation of the antimicrobial activity of Justicia gendarussa extracts.
• Exploring the potential of Justicia gendarussa as a source of valuable medicinal compounds.
• Contribution to the sustainable cultivation and conservation of this medicinal plant.

Zusammenfassung der Kapitel (Chapter Summaries)

• The introduction provides background information on Justicia gendarussa, outlining its medicinal importance and the significance of in vitro propagation techniques. The chapter also defines the specific aims and objectives of the study.
• The review of literature comprehensively discusses the botanical characteristics, medicinal properties, and previous research on Justicia gendarussa. It highlights the importance of this plant in traditional medicine and the need for further research to explore its full potential.
• The materials and methods section details the experimental procedures employed in the study. It describes the plant material used, the media formulations prepared, the experimental systems employed, and the statistical analysis performed. This section also includes descriptions of the equipment and techniques utilized in the study.
• The results and discussion section presents the findings of the study. It analyzes the effectiveness of different media formulations in inducing callus formation and direct organogenesis in Justicia gendarussa. This section also evaluates the antimicrobial activity of the plant extracts and discusses the implications of the findings for future research and applications.

Schlüsselwörter (Keywords)

The study centers on Justicia gendarussa, callus induction, direct organogenesis, in vitro propagation, medicinal plants, antimicrobial activity, Kerala, and plant tissue culture. These keywords encapsulate the core themes and research focus of the work.

• Hochschule: Mar Augusthinose College
• Note: 1.5
• Autoren: Dr. Prem Jose Vazhacharickal (Autor:in), Elizabeth Cherian (Autor:in), Noby Mathew (Autor:in)
• Erscheinungsjahr: 2017
• Seiten: 39
• Katalognummer: V378387
• ISBN (eBook): 9783668616318
• ISBN (Buch): 9783668616325
• Sprache: Englisch
• Schlagworte: Optimization, Formulation, Callus, Induction, Organogenesis, Burm