The successful in developing a straightforward preparative route in biomaterial composite fabrication with the great promising Umbilical Cord Matrix derived mesenchymal stem cells (MSCs) known as Wharton’s Jelly also well known as fetal MSC.
WJMSC has been created a reliable modernist and briskest innovative research method especially in manipulation of Graphene Oxide (GO) as a new biomaterials loaded with an ample advantages in regard of synthetic materials that applicable for a replacement or regenerating a living system.
Bio-interface in mesenchymal stem cell certainly brighten the hope of all stripes scientist researchers in aspiration of biomaterial application whilst can impact the benefit in the fields of biomedical, biotechnology, bioengineering, pharmacology and biosensors yet the finding remain a number of challenges in the fast-growing field.
The purpose of this review, we enumerated the key aspect an earmark that will be focused on GO functionalization, and GO-WJMSC characterization, accordingly to GO-WJMSC application in enhanced the proliferation, and multipotency for a brighter future potential of GO-stem cells applicability.
Table of Contents
1. INTRODUCTION
2. Graphene Oxide
3. Graphene Oxide properties
4. Wharton’s Jelly Derived Mesenchymal Stem Cell
5. GO-based composite functionalization
5.1 Cell biological application
5.2 Biomedical device application
5.3 Biosensor application
5.4 Bio-environment and energy application
5.5 Drug carrier application
6. GO-WJMSC characterization analysis
6.1 Morphology
6.2 Viability
6.3 Phenotypically and purity
6.4 Multi-potency
7. GO-stem cell future potential applicability
8. Conclusion
Objectives and Core Topics
This review aims to explore the advancements in the functionalization and characterization of Graphene Oxide (GO)-based composites and their critical role in enhancing the therapeutic efficacy, proliferation, and multipotency of human Wharton’s Jelly derived Mesenchymal Stem Cells (hWJMSCs) for regenerative medicine.
- Mechanisms of GO surface functionalization and biocompatibility.
- Impact of GO-based composites on stem cell behavior, including adhesion and proliferation.
- Applications of GO in biomedical engineering, drug delivery, and biosensing.
- Strategies for optimizing stem cell differentiation towards specific lineages.
- Future potentials of GO-based scaffolds in tissue engineering and clinical implantation.
Excerpt from the Book
5. GO-based composite functionalization
Along the lines of GO modified functionalization method, strong emphasize in the superior properties in completely package is enumerated for instance as a unique functional group applicable including epoxide, carboxyl and hydroxyl group may lead to serve a larger surface area, mechanical stability, electrostatic interaction capacity and optical properties which promise its great characteristic among other materials ideally expended commitment on GO application as scaffold either substrate including in vast field of biotechnology, biomedical, biosensor and stretched a head upon in energy platform also in lithium batteries and as a super capacitors (Becerril et al. 2008; Li et al. 2015: Sharma et al., 2016).
The most common GO synthesis is manufactured using first principal by Hummers method implied the oxidation of graphite treated with potassium permanganate and sulfuric acid (Gu et al., 2013: Hummers et al., 1958) nonetheless GO has been considered as one of a low-cost material manufacturing which attract the wide world scientist to give full effort on GO investigation (Dubey et al., 2015: Singh etal., 2009: Sharma et al., 2016).
Generally, there are several common on a solid support substrate have been demonstrated in tissue engineering for instances including glass, polymers, gold or silicon, and thin coatings such as poly-L-lysine, agarose or polyacrylamide (Ghaemi et al., 2013) to a slight variation in emphasize the substrate functionalization through dip or spin coating, plasma treatment, surface grafting, self-assembled monolayer (SAM) formation and micro fluidics approaches.
Summary of Chapters
1. INTRODUCTION: Discusses the emergence of biomaterial-stem cell technology and the role of Graphene Oxide (GO) in advancing stem cell research.
2. Graphene Oxide: Details the synthesis methods of GO, including exfoliation and oxidation techniques developed for efficient production.
3. Graphene Oxide properties: Highlights the unique physicochemical characteristics of GO, such as its amphiphilicity, mechanical strength, and electrical conductivity.
4. Wharton’s Jelly Derived Mesenchymal Stem Cell: Reviews the biological characteristics and therapeutic advantages of MSCs derived from Wharton’s Jelly.
5. GO-based composite functionalization: Outlines methodologies for functionalizing GO and its applications in biological, medical, and environmental fields.
6. GO-WJMSC characterization analysis: Analyzes the interactions between GO-based composites and stem cells, focusing on morphology, viability, purity, and differentiation capacity.
7. GO-stem cell future potential applicability: Discusses recommended future research directions to optimize GO-based biocomposites for clinical regenerative medicine.
8. Conclusion: Summarizes the current standing of GO modification in stem cell cultivation and provides a forward-looking perspective on its role in future therapy.
Keywords
Graphene Oxide, Biomaterials, Mesenchymal Stem Cells, Wharton’s Jelly, Functionalization, Tissue Engineering, Cell Adhesion, Biocompatibility, Surface Modification, Regenerative Medicine, Stemness, Nanotechnology, Cell Differentiation, Scaffold, Bio-interface.
Frequently Asked Questions
What is the primary focus of this work?
This work focuses on the modification of Graphene Oxide-based composites and their application in enhancing the biological performance, proliferation, and multipotency of human Wharton’s Jelly derived Mesenchymal Stem Cells.
What are the central thematic fields covered?
The central fields include biomaterials science, stem cell engineering, surface functionalization, regenerative medicine, and the specific application of Graphene Oxide in biomedical devices and drug delivery.
What is the primary research goal?
The primary goal is to evaluate how GO-based composite functionalization can improve stem cell behavior, specifically for applications in tissue engineering and clinical therapy.
Which scientific methods are analyzed?
The text reviews various synthesis methods such as the Hummers method, and surface modification techniques including dip/spin coating, plasma treatment, and self-assembled monolayers for bio-interface improvement.
What topics are discussed in the main body?
The main body covers the unique properties of Graphene Oxide, the biology of WJMSCs, detailed functionalization methods for various biomedical applications, and comprehensive characterization analyses of GO-stem cell interactions.
Which keywords best characterize the work?
Key terms include Graphene Oxide, Mesenchymal Stem Cells, Wharton’s Jelly, functionalization, tissue engineering, biocompatibility, and regenerative medicine.
How does Graphene Oxide improve the viability of MSCs?
Graphene Oxide improves viability through its unique surface chemistry, which enhances protein adsorption and electrostatic interactions, thereby providing a more favorable environment for stem cell adhesion and growth compared to standard culture plates.
What is the role of the Hummer's method in this context?
The Hummer's method is the primary technique used for the chemical synthesis of Graphene Oxide from graphite, which is essential for creating the material used in these stem cell scaffolds.
Why is Wharton's Jelly considered a promising stem cell source?
Wharton's Jelly derived MSCs are highly valued because they are readily available, non-invasively harvested, ethically obtained, and exhibit superior proliferation and multipotent characteristics.
- Arbeit zitieren
- Y. Umul Hanim (Autor:in), 2017, Modifications of Graphene Oxide-based composites biomaterial promote human Wharton’s Jelly derived Mesenchymal Stem Cells. Functionalization and characterization, München, GRIN Verlag, https://www.grin.com/document/378731
