Leseprobe
Table of contents
Abstract
Glossary
1 Introduction: Oligonucleotide Synthesis
2 Objective
3 Procedures and Materials
3.1 Chemistry
3.1.1 Methods and Materials utilized
3.1.2 Syntheses
3.2 Biology
3.2.1 Methods and Materials utilized
3.2.2 Primer Synthesis
3.2.3 Plasmid Mini Prep
3.2.4 Agarose Gel Electrophoresis
3.2.5 Polymerase Chain Reaction
3.2.6 Ligation
3.2.7 Transformation
3.2.8 Colony PCR
3.2.9 Protein Expression
3.2.10 Protein Purification
3.2.11 SDS-PAGE
3.2.12 Protein Digest
3.2.13 Protein Click Reaction
4 Results and Discussion
4.1 Agarose Gel Electrophoresis
4.2 SDS PAGE
4.3 Fluorescence Spectroscopy
4.4 Mass Spectrometry
4.4.1 MALDI-TOF
4.4.2 LC / MS
5 References
Ende der Leseprobe aus 47 Seiten
- Arbeit zitieren
- Anonym, 2014, Unnatural Amino Acid Incorporation and Click Chemistry, München, GRIN Verlag, https://www.grin.com/document/416795
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