We carried out research out with the topic of tissue culture technology for the large scale and sustainable production of quality planting material of elite medicinal plants and developped a database of Indian medicinal plants with the following objectives: (1) Establishment of tissue culture protocol for seven medicinal plants. (2) Standardizing the physicochemical conditions for large-scale in vitro multiplication. (3) Standardization of various media types and growth regulators. (4) Studying the competency of in vitro plants to sustain the subculture durations. (5) Collection of data of medicinal plants for creating a database (an online webpage). (6) Compiling and designing of database. (7) Publishing the database online.
Medicinal plants are the great natural resources but due to lack of knowledge, arbitrary use and lack of conservation measures many important medicinal plant species are becoming extinct, endangered and threatened. In the present research, micropropagation studies were carried out on ten medicinal plants, showed best respond in seven medicinal plants among them.
Callus cultures and shoot cultures were successfully initiated on basal MS media supplemented with different plant growth regulators (2,4-D, NAA, IAA and BAP) of various concentrations. These can be beneficial aspect in future as for exporting or making available the plants to farmers of superior genotype. This would also facilitate in metabolite extraction and supply in drug industries.
An online static database in the form of a web site was also created named as “MedDBase”. This database facilitates the medicinal as well as tissue culture information of all Indian medicinal plants. The list contained Indian medicinal plants more than 5000, is retrieved from the NMPB site and arranged in Alphabetical order. This online database will help the scientific community to keep themselves updated with the research and development work being carried out for a particular medicinal plant. This will also help in reducing duplication/ repetition of same work.
Inhaltsverzeichnis (Table of Contents)
- ABSTRACT
- INTRODUCTION
- Objectives
- Plant species selected for present research
- Aloe barbadensis MILL.
- Euphorbia hirta L.
- Kalanchoe pinnata (lam.) pers.
- Mimosa pudica L.
- Rauvolfia serpentina (L.) BENTH. EX KURZ.
- Saraca asoca (ROXB.) DE WILDE
- Trigonella foenum-graecum L.
- REVIEW OF LITERATURE
- Aloe barbadensis MILL.
- Euphorbia hirta L.
- Kalanchoe pinnata (lam.) pers.
- Mimosa pudica L.
- Rauvolfia serpentina (L.) BENTH. EX KURZ.
- Saraca asoca (ROXB.) DE WILDE
- Trigonella foenum-graecum L.
- MATERIAL AND METHODS
- Establishment of in vitro cultures of elite medicinal plants
- Plant Material
- Surface sterilization
- Establishment of in vitro cultures
- Standardizing physico-chemical conditions
- Statistical Analysis
- Developing the Medicinal Plant Database
- Data collection
- Database Design
- Establishment of in vitro cultures of elite medicinal plants
- RESULTS AND DISCUSSION
- Establishment of in vitro cultures of elite medicinal plants
- Aloe barbadensis MILL.
- Euphorbia hirta L.
- Kalanchoe pinnata (lam.) pers.
- Mimosa pudica L.
- Rauvolfia serpentina (L.) BENTH. EX KURZ.
- Saraca asoca (ROXB.) DE WILDE
- Trigonella foenum-graecum L.
- Developing the Medicinal Plant Database
- Establishment of in vitro cultures of elite medicinal plants
- REFERENCES
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This research aims to address the decline of medicinal plant species due to overexploitation and lack of conservation efforts. The study focuses on the micropropagation of ten medicinal plants, with a particular emphasis on achieving successful callus and shoot cultures through the use of plant growth regulators. The research also involves the development of a comprehensive online database, MedDBase, to provide information on both medicinal and tissue culture aspects of Indian medicinal plants. This database aims to facilitate research, prevent duplication of efforts, and make vital information readily accessible to the scientific community.
- Micropropagation of endangered medicinal plants
- In vitro culture techniques for callus and shoot initiation
- Role of plant growth regulators in micropropagation
- Development of an online medicinal plant database (MedDBase)
- Conservation and sustainable utilization of medicinal plants
Zusammenfassung der Kapitel (Chapter Summaries)
The introduction of this work details the current challenges facing medicinal plant conservation, highlighting the importance of micropropagation as a sustainable solution. The research focuses on seven specific medicinal plants, outlining their individual properties and potential applications. This section also provides a clear overview of the research objectives and methodologies employed.
The "Review of Literature" chapter comprehensively examines existing research on the chosen medicinal plants, focusing on their individual properties, cultivation techniques, and past research efforts in micropropagation. The chapter delves into specific aspects like callus initiation and shoot development, outlining the challenges and successes of previous studies.
The "Material and Methods" chapter provides detailed information on the experimental procedures employed. This includes descriptions of the plant material used, sterilization methods, and the specific growth media and plant growth regulators utilized for in vitro culture. The development of the MedDBase database is also explained, highlighting the data collection methods and database design.
The "Results and Discussion" chapter presents the experimental findings, focusing on the successful establishment of callus and shoot cultures for the seven selected medicinal plants. The chapter analyzes the impact of different plant growth regulators on callus formation and shoot regeneration. It also discusses the development and functionality of the MedDBase, showcasing its features and potential benefits for the scientific community.
Schlüsselwörter (Keywords)
This research utilizes a range of keywords that highlight its focus on sustainable plant utilization, micropropagation techniques, and the development of online resources. Key terms include: medicinal plants, micropropagation, in vitro culture, plant growth regulators, callus initiation, shoot regeneration, online database, MedDBase, conservation, sustainable utilization.
- Quote paper
- Arvind Kumar (Author), 2014, Augmenting medicinal plant research through in vitro and in silico approach, Munich, GRIN Verlag, https://www.grin.com/document/424259
