We carried out research out with the topic of tissue culture technology for the large scale and sustainable production of quality planting material of elite medicinal plants and developped a database of Indian medicinal plants with the following objectives: (1) Establishment of tissue culture protocol for seven medicinal plants. (2) Standardizing the physicochemical conditions for large-scale in vitro multiplication. (3) Standardization of various media types and growth regulators. (4) Studying the competency of in vitro plants to sustain the subculture durations. (5) Collection of data of medicinal plants for creating a database (an online webpage). (6) Compiling and designing of database. (7) Publishing the database online.
Medicinal plants are the great natural resources but due to lack of knowledge, arbitrary use and lack of conservation measures many important medicinal plant species are becoming extinct, endangered and threatened. In the present research, micropropagation studies were carried out on ten medicinal plants, showed best respond in seven medicinal plants among them.
Callus cultures and shoot cultures were successfully initiated on basal MS media supplemented with different plant growth regulators (2,4-D, NAA, IAA and BAP) of various concentrations. These can be beneficial aspect in future as for exporting or making available the plants to farmers of superior genotype. This would also facilitate in metabolite extraction and supply in drug industries.
An online static database in the form of a web site was also created named as “MedDBase”. This database facilitates the medicinal as well as tissue culture information of all Indian medicinal plants. The list contained Indian medicinal plants more than 5000, is retrieved from the NMPB site and arranged in Alphabetical order. This online database will help the scientific community to keep themselves updated with the research and development work being carried out for a particular medicinal plant. This will also help in reducing duplication/ repetition of same work.
Table of Contents
1 ABSTRACT
2 INTRODUCTION
2.1 Objectives
2.2 Plant species selected for present research
2.2.1 Aloe barbadensis MILL.
2.2.2 Euphorbia hirta L.
2.2.3 Kalanchoe pinnata (lam.) pers.
2.2.4 Mimosa pudica L.
2.2.5 Rauvolfia serpentina (L.) BENTH. EX KURZ
2.2.6 Saraca asoca (ROXB.) DE WILDE
2.2.7 Trigonella foenum-graecum L.
3 REVIEW OF LITERATURE
3.1.1 Aloe barbadensis MILL.
3.1.2 Euphorbia hirta L.
3.1.3 Kalanchoe pinnata (lam.) pers.
3.1.4 Mimosa pudica L.
3.1.5 Rauvolfia serpentina (L.) BENTH. EX KURZ
3.1.6 Saraca asoca (ROXB.) DE WILDE
3.1.7 Trigonella foenum-graecum L.
4 MATERIAL AND METHODS
4.1 Establishment of in vitro cultures of elite medicinal plants
4.1.1 Plant Material
4.1.2 Surface sterilization
4.1.3 Establishment of in vitro cultures
4.1.4 Standardizing physico-chemical conditions
4.1.5 Statistical Analysis
4.2 Developing the Medicinal Plant Database
4.2.1.1 Data collection
4.2.1.2 Database Design
5 RESULTS AND DISCUSSION
5.1 Establishment of in vitro cultures of elite medicinal plants
5.1.1 Aloe barbadensis MILL.
5.1.2 Euphorbia hirta L.
5.1.3 Kalanchoe pinnata (lam.) pers.
5.1.4 Mimosa pudica L.
5.1.5 Rauvolfia serpentina (L.) BENTH. EX KURZ
5.1.6 Saraca asoca (ROXB.) DE WILDE
5.1.7 Trigonella foenum-graecum L.
5.2 Developing the Medicinal Plant Database
6 REFERENCES
Objectives and Research Themes
The primary objective of this research is to establish micropropagation protocols for seven specific medicinal plants to ensure sustainable production and to develop a comprehensive online database of Indian medicinal plants. By leveraging tissue culture technology, the study aims to reduce the pressure on wild populations and provide the scientific community with an updated, accessible resource for research and development to prevent the duplication of efforts.
- Micropropagation and tissue culture optimization for elite medicinal plants.
- Standardization of physicochemical conditions and growth regulators for in vitro plant growth.
- Development of a comprehensive, alphabetized static web database (MedDBase).
- Sustainable utilization and conservation strategies for high-value medicinal species.
Excerpt from the Book
2.2.3 Kalanchoe pinnata (lam.) pers.
Kalanchoe pinnata commonly known as Patharchatta is a genus of Bryophyllum of the family Crassulaceae is a valuable medicinal as well as ornamental plant. It is a genus of about 125 species of tropical region that grows 3-5 feet high. These plants are cultivated as ornamental house plants and rock or succulent garden plants (Kulka 2006). It has a definite ornamental value (Zamora et al., 1998) because of its beautiful inflorescence. It is a popular houseplant and has become naturalized in temperate regions of Asia, the Pacific and Caribbean, Australia, New Zealand, West Indies, Macaronesia, Mascarenes, Galapagos, Melanesia, Polynesia and Hawaii (Zamora et al., 1998).
It is used as ornamental potted plant around the globe. Along with its ornamental values these species also contain a lot of medicinal values (Quisumbing 1951; Joseph et al., 2011). In traditional medicine, these species have been used to treat ailments such as infections, rheumatism and inflammation. Kalanchoe extracts also have immunosuppressive effects.
Summary of Chapters
1 ABSTRACT: This chapter provides an overview of the micropropagation studies on ten medicinal plants and the creation of an online database to support the scientific community.
2 INTRODUCTION: This section details the significance of India's biodiversity and the critical need for conservation and sustainable trade practices in the medicinal plants sector.
3 REVIEW OF LITERATURE: This chapter compiles existing research on the propagation and medicinal properties of selected plants, emphasizing the limitations of current documentation.
4 MATERIAL AND METHODS: This section describes the techniques used for in vitro culture establishment, sterilization, and the structural design of the MedDBase website.
5 RESULTS AND DISCUSSION: This chapter presents the data obtained from the micropropagation experiments and discusses the effectiveness of the developed medicinal plant database.
6 REFERENCES: This section lists all scientific literature and sources cited throughout the research.
Keywords
Medicinal plants, Micropropagation, Tissue culture, Callus induction, Shoot regeneration, MedDBase, In vitro, Plant growth regulators, Conservation, Sustainability, Biodiversity, Phytochemicals, Indian flora, Biotechnology, Drug industry.
Frequently Asked Questions
What is the core focus of this research?
The research focuses on optimizing micropropagation protocols for seven medicinal plants and creating an organized online database for medicinal plants in India.
Which thematic areas are covered in this study?
The study spans plant biotechnology, specifically tissue culture (micropropagation), medicinal plant conservation, and database design for scientific information sharing.
What is the primary goal of the research?
The main goal is to promote sustainable plant production through tissue culture and to provide a centralized, accessible online resource to prevent repetitive research and assist practitioners.
Which scientific method is utilized for propagation?
The researchers utilized various in vitro pathways, including axillary bud induction, callogenesis, and organogenesis, using MS basal medium supplemented with specific growth regulators.
What topics are discussed in the main body?
The body covers the rationale for medicinal plant study, a review of existing literature, step-by-step laboratory methods for in vitro culture, and results regarding plant growth response.
What are the characterizing keywords of this work?
Key terms include medicinal plants, micropropagation, tissue culture, callus induction, and database development.
How was the database compiled?
Data was gathered from scientific journals and literature, stored in MS Access 2013, and then translated into HTML pages on a Google Site.
How does this study address plant extinction?
By establishing efficient laboratory protocols for cultivation, the research aims to reduce reliance on wild-harvesting and promote sustainable supply chains for medicinal plant industries.
Why are growth regulators significant in this study?
Growth regulators like BAP, NAA, and 2,4-D are essential in MS media to trigger specific responses such as shoot initiation or callus development in different explants.
What is the benefit of the created MedDBase?
It provides a single source for propagation and medicinal properties, helping researchers and students avoid duplicating work and keeping them updated on plant-specific findings.
- Quote paper
- Arvind Kumar (Author), 2014, Augmenting medicinal plant research through in vitro and in silico approach, Munich, GRIN Verlag, https://www.grin.com/document/424259
