This study was conducted at the Integrated Biotechnology Block, College of Agriculture, Vellayani, Thiruvananthapuram during 2015-2017. The objective of the study was isolation and sequencing of genes related to shattering viz. sh4 and qsh1 in weedy rice biotypes and characterization by expression profiling and phylogenetic analysis. The tBLASTx programme showed that the amplicon of size ~690bp was similar to shattering 4 (sh4) genes of Oryza populations, whereas the amplicon of size ~750bp belonged to qsh1 gene for putative transcription factor qsh1 of rice populations and mRNA of Oryza sativa japonica group.
The result clearly revealed that the gene responsible for shattering viz., sh4 and qsh1 are present in both weedy rice and cultivated variety Uma as evidenced from the PCR amplification of genomic DNA. However, the genes responsible for shattering investigated in the present study viz., sh4 and qsh1 were not expressed in any of the growth stages in cultivated rice variety Uma. The NCBI conserved Domain Search programme, showed that the qsh1 FW2 x RW2 sequence of amplicon at ~750bp (obtained by reaction using weedy rice flag leaf cDNA) belonged to homeobox kn domain. The sequence of the amplicons of qsh1 obtained was the partial fragments of the first ever qsh1 gene for putative transcription factor qsh1 to be isolated from Oryza sativa f. spontanea.
Phylogenetic tree constructed for sh4 FW2 x RW2 sequence, had highest similarity to shattering (sh4) gene of Oryza meridionalis voucher and also had relationship with Oryza rufipogon and Oryza sativa. However, the weedy rice qsh1 FW2 x RW2 query sequence was closely related to qsh1 gene of Oryza rufipogon, together with Oryza sativa indica group and Oryza sativa japonica group. Semi quantitative analysis with sh4 and qsh1 gene primers showed that the sh4 gene was expressed in flag leaf and grains and qsh1 gene was expressed only in the flag leaf. From this study it can be inferred that the gens responsible for shattering in weedy rice biotypes of Kerala viz. sh4 and qsh1 are present both in cultivated rice and weedy rice. However, the genes are expressed only in weedy rice during the flowering stage.
Inhaltsverzeichnis (Table of Contents)
- INTRODUCTION
- REVIEW OF LITERATURE
- MATERIALS AND METHODS
- RESULTS
- DISCUSSION
- SUMMARY
- REFERENCES
- APPENDICES
- LIST OF FIGURE CREDITS
- ABSTRACT
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This thesis aims to delve into the molecular characterization of shattering in weedy rice (Oryza sativa f. spontanea) biotypes found in Kerala, India. The research seeks to understand the genetic basis of shattering, a crucial trait for weed persistence and dispersal in rice.
- Molecular characterization of shattering in weedy rice
- Genetic diversity of shattering in weedy rice populations
- Identification of key genes responsible for shattering
- Implications for weed management and crop improvement
- Understanding the evolutionary dynamics of shattering in weedy rice
Zusammenfassung der Kapitel (Chapter Summaries)
- Introduction: This chapter provides a general introduction to the topic of weedy rice, its importance, and the significance of shattering in weed persistence. It highlights the challenges posed by weedy rice to rice cultivation and the need for understanding its genetic basis.
- Review of Literature: This chapter reviews existing knowledge on the molecular basis of shattering in rice, focusing on the genes and pathways involved. It discusses previous studies on weedy rice and its genetic diversity, as well as current strategies for weed management.
- Materials and Methods: This chapter details the experimental procedures used in the research, including the collection of weedy rice biotypes, DNA extraction, molecular marker analysis, and data analysis techniques. It outlines the methods used to assess the genetic diversity of shattering in the collected samples.
- Results: This chapter presents the findings of the research, including the genetic diversity observed in the weedy rice populations, the identification of key genes associated with shattering, and the relationships between genotype and phenotype.
Schlüsselwörter (Keywords)
The study focuses on keywords such as weedy rice, Oryza sativa f. spontanea, shattering, genetic diversity, molecular markers, gene mapping, weed management, and crop improvement. These terms encompass the core concepts and research focuses of the thesis.
- Quote paper
- Shelvy Sree (Author), 2017, Molecular characterization of shattering in weedy rice biotypes of Kerala, Munich, GRIN Verlag, https://www.grin.com/document/470265
