This work is a collection of studies concerning human sustainability. We were proud for having Postgrad Colloquium 2019, Faculty of Health Sciences Universiti Sultan Zainal Abidin (UniSZA). It was held on 30th April 2019 at Academic Building UniSZA Main Campus Kuala Nerus, Terengganu, Malaysia. Total of 24 students including 15 students from Laboratory Based Research category and 9 students from Community Based Research category of Faculty Health Sciences and Faculty of Medicine were participated. We congratulated to the winners Omar Mahmoud Said Alshajrawi from Faculty of Medicine, Masters student for the Laboratory Based Research category and Mohd Ibrahim Abdullah from Faculty of Health Sciences, Doctorate student for the Community Based Research category. Both of you were pretty amazing. See again next year on 2020 to all researchers.
At a glance
- Tests 260 clinical isolates from Hospital Sultanah Nur Zahirah against 26 antibiotics
- Compares five PkAMA1 expression clones spanning domains I, II, and III
- Uses five probiotic Lactobacillus strains against four periodontal pathogen strains
- Evaluates Trigona honey against Pseudomonas aeruginosa and Streptococcus pyogenes biofilms
Contents
CLONING AND CHATACTERIZATION OF ALPHA ENOLASE GENE OF 6A STREPTOCOCCUS PNEUMONIA TOWARDS INDUCTION OF NETOSIS
CONSTRUCTION OF FIVE EXPRESSION CLONES FOR PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN 1 (PKAMA1) USING PICHIA PASTORISEXPRESSION SYSTEM
ISOLATION AND CHARACTERISTICS OF METHICILLIN‐RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) AND METHICILLIN‐SUSCEPTIBLE STAPHYLOCOCCUS AUREUS (MSSA) CLINICAL ISOLATES IN TERENGGANU
RECOMBINANT PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN‐1 (PKAMA1) EXPRESSION FOR THE PHAGE‐DISPLAYED BINDING PEPTIDES SELECTION
THE POTENTIAL EFFECT OF MALAYSIAN TRIGONA HONEY ON ENTERIC AND RESPIRATORY INFECTIONS BY USING WISTAR RATS MODEL
THE GENOTYPIC AND ALLELIC CHARACTERIZATION OF VEGF PROMOTER (RS833061) VARIATION(S) AND THEIR ASSOCIATION WITH LIPID PROFILE, ANTHROPOMETRIC INDICES AND PHYSICAL ACTIVITY LEVELS AMONG MALAY OBESE
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF MALAYSIAN TRIGONA HONEY AGAINST PSEUDOMONAS AERUGINOSA AND STREPTOCOCCUS PYOGENES
THE ROLE OF SOX 10 GENE IN THE DEVELOPMENT OF GLIOBLASTOMA MULTIFORME
BIOFILM INHIBITION OF PERIODONTAL PATHOGEN BY PROBIOTIC STRAINS: GENE
AN ASSOCIATION STUDY ON GENETIC POLYMORPHISM OF HBB GENE AMONG BETA‐THALASEMIA MINOR PATIENTS
BIOACTIVE COMPOUNDS, NUTRITIONAL VALUE, AND POTENTIAL HEALTH BENEFITS OF INDIGENOUS DURIAN (DURIO ZIBETHINUS MURR.): A REVIEW
UNRAVELLING THE EFFECTS OF SOLUBLE DIETARY FIBRE SUPPLEMENTATION ON ENERGY INTAKE AND PERCEIVEDSATIETY IN HEALTHY ADULTS: EVIDENCE FROMSYSTEMATIC REVIEW AND META‐ANALYSIS OF RANDOMISED‐CONTROLLED TRIALS
DETERMINATION OF SOX GENE EXPRESSION IN BRAIN TUMOR IN EAST COAST MALAYSIA
GENETIC ASSOCIATION OF VARIATIONS IN HIF1 (RS11549465) AND NFKΒ1 (RS28362491) WITH ANTHROPOMETRIC INDICES AND LIPID PROFILE IN MALAY OBESE ATTRIBUTES.
IN VITRO ANTIBACTERIAL PROPERTIES OF ETLINGERA ELATIOR FLOWER EXTRACTS AGAINST ACNE‐INDUCING BACTERIA: PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS.
THE EFFECTS OF CALORIE RESTRICTION ON COGNITIVE RECOVERY AMONG TRAUMATIC BRAIN INJURY PATIENTS
STUDY PROPOSAL: ASSOCIATION BETWEEN DIETARY PATTERNS AND CORONARY HEART DISEASE (CHD) RISK FACTORS AMONG MALAYSIAN POPULATION
PREVALENCE OF LATENT TUBERCULOSIS INFECTION AND ITS ASSOCIATED FACTORS AMONG DIABETIC PATIENTS AT PRIMARY HEALTH CARE IN TERENGGANU
PREVALENCE OF CYBERBULLYING VICTIMS AND ITS ASSOCIATED FACTORS AMONG FORM 2 AND FORM 4 STUDENTS IN KUALA TERENGGANU, MALAYSIA
STUDY PROPOSAL: DETERMINATION OF PROANTHOCYANIDIN CONTENTS IN MELON MANIS TERENGGANU (CUCUMIS MELO L.) AND THERAPEUTIC APPLICATION AMONG KNEE OSTEOARTHRITIS PATIENTS
THE RELATIONSHIP OF FRUIT INTAKE WITH BRAIN DERIVED NEUROTROPHIC FACTOR (BDNF) PROTEIN LEVEL AMONG ADOLESCENTS IN TERENGGANU
STUDY PROPOSAL: PREVALENCE AND RISK FACTORS OF HYPERTENSION AMONG ADULTS IN TERENGGANU’S FELDA
THE CONCEPT OF WELL‐BEING AMONG HEMODIALYSIS CHRONIC KIDNEY PATIENTS: A CORRELATION STUDY
STUDY PROPOSAL: DEVELOPMENT OF DIETARY RISK CALCULATOR TO PREDICT CORONARY
Purpose and Topics
This edited collection presents health-science research and study proposals from a 2019 postgraduate colloquium at Universiti Sultan Zainal Abidin. Its work examines biomedical mechanisms, infectious disease, nutrition, health behavior, and patient well-being through laboratory studies, clinical data, surveys, reviews, and proposed research.
- Pathogens, antimicrobial resistance, and biofilm inhibition
- Genetic variation and gene expression in obesity and brain tumors
- Nutrition, dietary fiber, honey, fruit, and health outcomes
- Clinical and community health research in Terengganu and Malaysia
- Research methods including molecular assays, case-control studies, and systematic review
Excerpt from the Book
Antibacterial and antibiofilm activities of Malaysian Trigona honey
The antibacterial activity of honey has been researched scientifically and used medicinally for many years. Most of the research works conducted focused on honey produced by honey bee and very few on the honey from stingless bees. Rising figure of therapeutic drug resistivity and low susceptibility by bacteria and other microbes and the resulting side effect of some pharmaceutical products, leads to the use of natural remedies such as honey. The use of natural remedies as a form of alternative therapy without much consideration to factors such as the chemical properties, pharmacological properties and compatibility with theories may result in worsening the condition rather than curing (Seeley, 2009). In Malaysia, there is lack of enough available data on the therapeutic value of stingless bee honey, which is highly needed since the stingless bee honey is readily available and utilized by the dwellers. Objective: This study aimed to investigate the antibacterial and antibiofilm activities of Trigona honey against Pseudomonas aeruginosa and Streptococcus pyogenes.
The antimicrobial and anti-biofilm activities were examined by agar well diffusion assays, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill curve, biofilm formation in 96-well plates and scanning electron microscope.
Agar diffusion assay showed that Trigona honey to possess highest total antibacterial activity against Pseudomonas aeruginosa and Streptococcus pyogenes, 25.2±0.6mm and 26.7±1.0mm respectively. Trigona honey possessed the lowest MIC value against Pseudomonas aeruginosa and Streptococcus pyogenes was 20% (w/v) and MBC was 25% (w/v). Also, MIC50 was between 10%-12.5% (w/v) and MIC90 was between 20%-25% (w/v) concentration of honey for both bacteria. In time-kill curve Trigona honey was inhibited P.aeruginosa and S.pyogenes in a 3 log10 at 18 hours, and total viable counts (TVCs) were killed after 24 hours at honey concentration of 25%. In biofilm degradation assay Trigona honey was degraded 70% of Pseudomonas aeruginosa and 68% of Streptococcus pyogenes biofilm. Also in biofilm inhibition assay Trigona honey was inhibited 91% of Pseudomonas aeruginosa and 89% of Streptococcus pyogenes biofilms.
Chapter Summaries
CLONING AND CHATACTERIZATION OF ALPHA ENOLASE GENE OF 6A STREPTOCOCCUS PNEUMONIA TOWARDS INDUCTION OF NETOSIS: This study amplifies and clones the S. pneumoniae Eno1 gene into a plasmid, laying groundwork for mutation and later testing of NETosis and bacterial dissemination.
CONSTRUCTION OF FIVE EXPRESSION CLONES FOR PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN 1 (PKAMA1) USING PICHIA PASTORISEXPRESSION SYSTEM: Five PkAMA1 domain constructs were cloned and confirmed for future recombinant protein expression in yeast.
ISOLATION AND CHARACTERISTICS OF METHICILLIN‐RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) AND METHICILLIN‐SUSCEPTIBLE STAPHYLOCOCCUS AUREUS (MSSA) CLINICAL ISOLATES IN TERENGGANU: Clinical isolates were assessed for antibiotic resistance, resistance phenotypes, and clinical characteristics.
RECOMBINANT PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN‐1 (PKAMA1) EXPRESSION FOR THE PHAGE‐DISPLAYED BINDING PEPTIDES SELECTION: The study expresses recombinant PkAMA1 and describes a phage-display approach to select binding peptides.
THE POTENTIAL EFFECT OF MALAYSIAN TRIGONA HONEY ON ENTERIC AND RESPIRATORY INFECTIONS BY USING WISTAR RATS MODEL: A rat model examines honey administration in bacterial infections, with tissue, immune, oxidative-stress, and blood measurements.
THE GENOTYPIC AND ALLELIC CHARACTERIZATION OF VEGF PROMOTER (RS833061) VARIATION(S) AND THEIR ASSOCIATION WITH LIPID PROFILE, ANTHROPOMETRIC INDICES AND PHYSICAL ACTIVITY LEVELS AMONG MALAY OBESE: This cross-sectional study characterizes VEGF promoter variation and examines links with obesity measures, lipid profiles, and activity.
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF MALAYSIAN TRIGONA HONEY AGAINST PSEUDOMONAS AERUGINOSA AND STREPTOCOCCUS PYOGENES: Laboratory assays assess honey’s antibacterial and antibiofilm activity against two bacterial species.
THE ROLE OF SOX 10 GENE IN THE DEVELOPMENT OF GLIOBLASTOMA MULTIFORME: Using U-87 MG cells, the study examines SOX 10 knockdown, cell proliferation, and apoptosis.
BIOFILM INHIBITION OF PERIODONTAL PATHOGEN BY PROBIOTIC STRAINS: GENE: The study tests Lactobacillus strains and their supernatants against periodontal pathogen biofilms over three time points.
AN ASSOCIATION STUDY ON GENETIC POLYMORPHISM OF HBB GENE AMONG BETA‐THALASEMIA MINOR PATIENTS: Sequencing and association analyses assess HBB polymorphisms in referral cases and controls.
BIOACTIVE COMPOUNDS, NUTRITIONAL VALUE, AND POTENTIAL HEALTH BENEFITS OF INDIGENOUS DURIAN (DURIO ZIBETHINUS MURR.): A REVIEW: This review summarizes nutritional, bioactive, and volatile compounds reported in durian varieties from Southeast Asia.
UNRAVELLING THE EFFECTS OF SOLUBLE DIETARY FIBRE SUPPLEMENTATION ON ENERGY INTAKE AND PERCEIVEDSATIETY IN HEALTHY ADULTS: EVIDENCE FROMSYSTEMATIC REVIEW AND META‐ANALYSIS OF RANDOMISED‐CONTROLLED TRIALS: A systematic review and meta-analysis evaluates fibre type, dose, and food matrix in relation to energy intake and satiety.
DETERMINATION OF SOX GENE EXPRESSION IN BRAIN TUMOR IN EAST COAST MALAYSIA: Patient records and archived tumor samples are used to describe case distribution and expression patterns of four SOX genes.
GENETIC ASSOCIATION OF VARIATIONS IN HIF1 (RS11549465) AND NFKΒ1 (RS28362491) WITH ANTHROPOMETRIC INDICES AND LIPID PROFILE IN MALAY OBESE ATTRIBUTES.: A case-control analysis examines two genetic variations in relation to obesity measures and lipid profiles.
IN VITRO ANTIBACTERIAL PROPERTIES OF ETLINGERA ELATIOR FLOWER EXTRACTS AGAINST ACNE‐INDUCING BACTERIA: PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS.: Three solvent extracts are assessed for antibacterial activity and chemical content against two acne-associated bacteria.
THE EFFECTS OF CALORIE RESTRICTION ON COGNITIVE RECOVERY AMONG TRAUMATIC BRAIN INJURY PATIENTS: A prospective observational case-control study relates calorie intake to cognitive and EEG measures in hospitalized patients.
STUDY PROPOSAL: ASSOCIATION BETWEEN DIETARY PATTERNS AND CORONARY HEART DISEASE (CHD) RISK FACTORS AMONG MALAYSIAN POPULATION: The proposal outlines a retrospective cohort study using medical records, dietary assessments, and physical-activity data.
PREVALENCE OF LATENT TUBERCULOSIS INFECTION AND ITS ASSOCIATED FACTORS AMONG DIABETIC PATIENTS AT PRIMARY HEALTH CARE IN TERENGGANU: A cross-sectional study uses tuberculin skin testing and participant data to examine latent tuberculosis and associated factors.
PREVALENCE OF CYBERBULLYING VICTIMS AND ITS ASSOCIATED FACTORS AMONG FORM 2 AND FORM 4 STUDENTS IN KUALA TERENGGANU, MALAYSIA: Questionnaires from two schools are used to study cyberbullying victimization and related characteristics.
STUDY PROPOSAL: DETERMINATION OF PROANTHOCYANIDIN CONTENTS IN MELON MANIS TERENGGANU (CUCUMIS MELO L.) AND THERAPEUTIC APPLICATION AMONG KNEE OSTEOARTHRITIS PATIENTS: The proposal sets out fruit-compound analysis, supplement development, and a randomized trial involving knee osteoarthritis patients.
THE RELATIONSHIP OF FRUIT INTAKE WITH BRAIN DERIVED NEUROTROPHIC FACTOR (BDNF) PROTEIN LEVEL AMONG ADOLESCENTS IN TERENGGANU: A preliminary cross-sectional study assesses fruit intake and serum BDNF in adolescents.
STUDY PROPOSAL: PREVALENCE AND RISK FACTORS OF HYPERTENSION AMONG ADULTS IN TERENGGANU’S FELDA: This proposal describes a community study of hypertension, lifestyle, dietary intake, and salt-related knowledge and practices.
THE CONCEPT OF WELL‐BEING AMONG HEMODIALYSIS CHRONIC KIDNEY PATIENTS: A CORRELATION STUDY: A questionnaire study explores well-being dimensions among hemodialysis patients at two treatment centers.
STUDY PROPOSAL: DEVELOPMENT OF DIETARY RISK CALCULATOR TO PREDICT CORONARY: The proposal describes development and validation of a dietary calculator intended to estimate coronary heart disease risk.
Keywords
health sciences, biomedical research, infectious disease, antimicrobial resistance, biofilms, gene expression, genetic polymorphism, obesity, nutrition, dietary fiber, clinical research, public health, Malaysia, Terengganu
Frequently Asked Questions
What is this book about?
It is a collection of health-science research abstracts and study proposals spanning laboratory, clinical, nutritional, and community-health topics.
What are the collection’s main subject areas?
Topics include infectious diseases, antimicrobial resistance, genetics, obesity, nutrition, brain tumors, cognitive recovery, and patient well-being.
What is the collection’s central research aim?
There is no single research question; each contribution sets out its own objective, ranging from gene characterization to population health assessment.
What research methods are represented?
Methods include laboratory assays, gene sequencing and expression analysis, cross-sectional and case-control studies, questionnaires, systematic review, and meta-analysis.
What does the main body cover?
It presents individual studies and proposals, including their research questions, methods, and—in completed studies—reported results.
Which keywords characterize the collection?
Health sciences, infectious disease, antimicrobial resistance, biofilms, gene expression, genetics, obesity, nutrition, clinical research, and public health.
How does the Trigona honey study assess antibiofilm activity?
It uses biofilm formation in 96-well plates and scanning electron microscopy, alongside agar diffusion, MIC, MBC, and time-kill assays.
What data were used in the Terengganu MRSA and MSSA study?
It assessed 260 non-duplicate clinical isolates collected at Hospital Sultanah Nur Zahirah between July 2016 and June 2017.
Which SOX genes were examined in brain tumor samples?
The study used quantitative real-time PCR to examine Sox6, Sox13, Sox9, and Sox8 in archived brain tumor samples.
What does the dietary risk calculator proposal plan to do?
It proposes developing a coronary heart disease risk calculator that includes dietary components, then testing its validity and reliability.
- Quote paper
- Mohd Hilmi Abu Bakar (Editor), 2019, Healthy and Beauty. Finding of current life science experiment for human sustainability, Munich, GRIN Verlag, https://www.grin.com/document/493733
