This work is a collection of studies concerning human sustainability. We were proud for having Postgrad Colloquium 2019, Faculty of Health Sciences Universiti Sultan Zainal Abidin (UniSZA). It was held on 30th April 2019 at Academic Building UniSZA Main Campus Kuala Nerus, Terengganu, Malaysia. Total of 24 students including 15 students from Laboratory Based Research category and 9 students from Community Based Research category of Faculty Health Sciences and Faculty of Medicine were participated. We congratulated to the winners Omar Mahmoud Said Alshajrawi from Faculty of Medicine, Masters student for the Laboratory Based Research category and Mohd Ibrahim Abdullah from Faculty of Health Sciences, Doctorate student for the Community Based Research category. Both of you were pretty amazing. See again next year on 2020 to all researchers.
Table of Contents
CLONING AND CHATACTERIZATION OF ALPHA ENOLASE GENE OF 6A STREPTOCOCCUS PNEUMONIA TOWARDS INDUCTION OF NETOSIS
CONSTRUCTION OF FIVE EXPRESSION CLONES FOR PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN 1 (PKAMA1) USING PICHIA PASTORISEXPRESSION SYSTEM
ISOLATION AND CHARACTERISTICS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) AND METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS AUREUS (MSSA) CLINICAL ISOLATES IN TERENGGANU
RECOMBINANT PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN-1 (PKAMA1) EXPRESSION FOR THE PHAGE-DISPLAYED BINDING PEPTIDES SELECTION
THE POTENTIAL EFFECT OF MALAYSIAN TRIGONA HONEY ON ENTERIC AND RESPIRATORY INFECTIONS BY USING WISTAR RATS MODEL
THE GENOTYPIC AND ALLELIC CHARACTERIZATION OF VEGF PROMOTER (RS833061) VARIATION(S) AND THEIR ASSOCIATION WITH LIPID PROFILE, ANTHROPOMETRIC INDICES AND PHYSICAL ACTIVITY LEVELS AMONG MALAY OBESE
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF MALAYSIAN TRIGONA HONEY AGAINST PSEUDOMONAS AERUGINOSA AND STREPTOCOCCUS PYOGENES
THE ROLE OF SOX 10 GENE IN THE DEVELOPMENT OF GLIOBLASTOMA MULTIFORME
BIOFILM INHIBITION OF PERIODONTAL PATHOGEN BY PROBIOTIC STRAINS: GENE
AN ASSOCIATION STUDY ON GENETIC POLYMORPHISM OF HBB GENE AMONG BETA-THALASEMIA MINOR PATIENTS
BIOACTIVE COMPOUNDS, NUTRITIONAL VALUE, AND POTENTIAL HEALTH BENEFITS OF INDIGENOUS DURIAN (DURIO ZIBETHINUS MURR.): A REVIEW
UNRAVELLING THE EFFECTS OF SOLUBLE DIETARY FIBRE SUPPLEMENTATION ON ENERGY INTAKE AND PERCEIVEDSATIETY IN HEALTHY ADULTS: EVIDENCE FROMSYSTEMATIC REVIEW AND META-ANALYSIS OF RANDOMISED-CONTROLLED TRIALS
DETERMINATION OF SOX GENE EXPRESSION IN BRAIN TUMOR IN EAST COAST MALAYSIA
GENETIC ASSOCIATION OF VARIATIONS IN HIF1 (RS11549465) AND NFKΒ1 (RS28362491) WITH ANTHROPOMETRIC INDICES AND LIPID PROFILE IN MALAY OBESE ATTRIBUTES.
IN VITRO ANTIBACTERIAL PROPERTIES OF ETLINGERA ELATIOR FLOWER EXTRACTS AGAINST ACNE-INDUCING BACTERIA: PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS.
THE EFFECTS OF CALORIE RESTRICTION ON COGNITIVE RECOVERY AMONG TRAUMATIC BRAIN INJURY PATIENTS
STUDY PROPOSAL: ASSOCIATION BETWEEN DIETARY PATTERNS AND CORONARY HEART DISEASE (CHD) RISK FACTORS AMONG MALAYSIAN POPULATION
PREVALENCE OF LATENT TUBERCULOSIS INFECTION AND ITS ASSOCIATED FACTORS AMONG DIABETIC PATIENTS AT PRIMARY HEALTH CARE IN TERENGGANU
PREVALENCE OF CYBERBULLYING VICTIMS AND ITS ASSOCIATED FACTORS AMONG FORM 2 AND FORM 4 STUDENTS IN KUALA TERENGGANU, MALAYSIA
STUDY PROPOSAL: DETERMINATION OF PROANTHOCYANIDIN CONTENTS IN MELON MANIS TERENGGANU (CUCUMIS MELO L.) AND THERAPEUTIC APPLICATION AMONG KNEE OSTEOARTHRITIS PATIENTS
THE RELATIONSHIP OF FRUIT INTAKE WITH BRAIN DERIVED NEUROTROPHIC FACTOR (BDNF) PROTEIN LEVEL AMONG ADOLESCENTS IN TERENGGANU
STUDY PROPOSAL: PREVALENCE AND RISK FACTORS OF HYPERTENSION AMONG ADULTS IN TERENGGANU’S FELDA
THE CONCEPT OF WELL-BEING AMONG HEMODIALYSIS CHRONIC KIDNEY PATIENTS: A CORRELATION STUDY
STUDY PROPOSAL: DEVELOPMENT OF DIETARY RISK CALCULATOR TO PREDICT CORONARY
Research Objectives and Themes
This volume provides an overview of contemporary life science research conducted within the Faculty of Health Sciences at Universiti Sultan Zainal Abidin (UniSZA). The primary goal is to disseminate findings from experimental and community-based research projects that address human sustainability, health challenges, and therapeutic advancements in Malaysia.
- Advancements in molecular biology, including gene cloning and expression studies.
- Investigation of the antimicrobial and therapeutic properties of natural resources like Trigona honey.
- Genetic studies focusing on disease associations, including obesity and thalassemia.
- Clinical and nutritional interventions aimed at improving public health outcomes and disease management.
Excerpt from the Book
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF MALAYSIAN TRIGONA HONEY AGAINST PSEUDOMONAS AERUGINOSA AND STREPTOCOCCUS PYOGENES
The antibacterial activity of honey has been researched scientifically and used medicinally for many years. Most of the research works conducted focused on honey produced by honey bee and very few on the honey from stingless bees. Rising figure of therapeutic drug resistivity and low susceptibility by bacteria and other microbes and the resulting side effect of some pharmaceutical products, leads to the use of natural remedies such as honey. The use of natural remedies as a form of alternative therapy without much consideration to factors such as the chemical properties, pharmacological properties and compatibility with theories may result in worsening the condition rather than curing (Seeley, 2009). In Malaysia, there is lack of enough available data on the therapeutic value of stingless bee honey, which is highly needed since the stingless bee honey is readily available and utilized by the dwellers. Objective: This study aimed to investigate the antibacterial and antibiofilm activities of Trigona honey against Pseudomonas aeruginosa and Streptococcus pyogenes.
Summary of Chapters
CLONING AND CHATACTERIZATION OF ALPHA ENOLASE GENE OF 6A STREPTOCOCCUS PNEUMONIA TOWARDS INDUCTION OF NETOSIS: This chapter details the successful amplification and cloning of the Eno1 gene to investigate its role in NETosis and bacterial dissemination.
CONSTRUCTION OF FIVE EXPRESSION CLONES FOR PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN 1 (PKAMA1) USING PICHIA PASTORISEXPRESSION SYSTEM: The research focuses on creating expression clones of PkAMA1 domains to facilitate future immunogenicity studies against malaria.
ISOLATION AND CHARACTERISTICS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) AND METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS AUREUS (MSSA) CLINICAL ISOLATES IN TERENGGANU: This study evaluates the antimicrobial resistance trends in S. aureus isolates collected from local clinical settings.
RECOMBINANT PLASMODIUM KNOWLESI APICAL MEMBRANE ANTIGEN-1 (PKAMA1) EXPRESSION FOR THE PHAGE-DISPLAYED BINDING PEPTIDES SELECTION: This section describes the expression of soluble recombinant PkAMA1 to aid in the discovery of binding peptides for drug development.
THE POTENTIAL EFFECT OF MALAYSIAN TRIGONA HONEY ON ENTERIC AND RESPIRATORY INFECTIONS BY USING WISTAR RATS MODEL: The study investigates the antioxidant and immunomodulatory effects of Trigona honey in rat models infected with E. coli and S. aureus.
THE GENOTYPIC AND ALLELIC CHARACTERIZATION OF VEGF PROMOTER (RS833061) VARIATION(S) AND THEIR ASSOCIATION WITH LIPID PROFILE, ANTHROPOMETRIC INDICES AND PHYSICAL ACTIVITY LEVELS AMONG MALAY OBESE: Researchers explore the relationship between VEGF promoter polymorphisms and obesity-related metabolic traits.
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF MALAYSIAN TRIGONA HONEY AGAINST PSEUDOMONAS AERUGINOSA AND STREPTOCOCCUS PYOGENES: This chapter demonstrates the high efficacy of Trigona honey in inhibiting bacterial growth and disrupting biofilms.
THE ROLE OF SOX 10 GENE IN THE DEVELOPMENT OF GLIOBLASTOMA MULTIFORME: An investigation into how the knockdown of the SOX 10 gene influences cell proliferation and apoptosis in brain tumor cells.
BIOFILM INHIBITION OF PERIODONTAL PATHOGEN BY PROBIOTIC STRAINS: GENE: This study explores the potential of probiotic Lactobacillus species to inhibit the biofilm formation of periodontal pathogens.
AN ASSOCIATION STUDY ON GENETIC POLYMORPHISM OF HBB GENE AMONG BETA-THALASEMIA MINOR PATIENTS: The research examines the potential link between specific HBB gene polymorphisms and thalassemia minor phenotypes.
BIOACTIVE COMPOUNDS, NUTRITIONAL VALUE, AND POTENTIAL HEALTH BENEFITS OF INDIGENOUS DURIAN (DURIO ZIBETHINUS MURR.): A REVIEW: A comprehensive review of the volatile compounds and health benefits associated with various durian varieties.
UNRAVELLING THE EFFECTS OF SOLUBLE DIETARY FIBRE SUPPLEMENTATION ON ENERGY INTAKE AND PERCEIVEDSATIETY IN HEALTHY ADULTS: EVIDENCE FROMSYSTEMATIC REVIEW AND META-ANALYSIS OF RANDOMISED-CONTROLLED TRIALS: A meta-analysis determining the optimal type and dose of dietary fibre to reduce energy intake.
DETERMINATION OF SOX GENE EXPRESSION IN BRAIN TUMOR IN EAST COAST MALAYSIA: An analysis of SOX family gene expression patterns in various types of brain tumors found in the region.
GENETIC ASSOCIATION OF VARIATIONS IN HIF1 (RS11549465) AND NFKΒ1 (RS28362491) WITH ANTHROPOMETRIC INDICES AND LIPID PROFILE IN MALAY OBESE ATTRIBUTES.: This chapter reports on the association between specific genetic variants and obesity-related metabolic risks.
IN VITRO ANTIBACTERIAL PROPERTIES OF ETLINGERA ELATIOR FLOWER EXTRACTS AGAINST ACNE-INDUCING BACTERIA: PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS.: The study evaluates the antibacterial potential of E. elatior extracts against bacteria responsible for acne.
THE EFFECTS OF CALORIE RESTRICTION ON COGNITIVE RECOVERY AMONG TRAUMATIC BRAIN INJURY PATIENTS: Research into the impact of calorie intake levels on the cognitive recovery of TBI patients.
STUDY PROPOSAL: ASSOCIATION BETWEEN DIETARY PATTERNS AND CORONARY HEART DISEASE (CHD) RISK FACTORS AMONG MALAYSIAN POPULATION: A research proposal outlining the study of dietary intakes and their contribution to CHD risk.
PREVALENCE OF LATENT TUBERCULOSIS INFECTION AND ITS ASSOCIATED FACTORS AMONG DIABETIC PATIENTS AT PRIMARY HEALTH CARE IN TERENGGANU: An analysis of the prevalence and risk factors of latent TB in a diabetic population.
PREVALENCE OF CYBERBULLYING VICTIMS AND ITS ASSOCIATED FACTORS AMONG FORM 2 AND FORM 4 STUDENTS IN KUALA TERENGGANU, MALAYSIA: This chapter identifies the prevalence and contributing factors of cyberbullying victimization among school students.
STUDY PROPOSAL: DETERMINATION OF PROANTHOCYANIDIN CONTENTS IN MELON MANIS TERENGGANU (CUCUMIS MELO L.) AND THERAPEUTIC APPLICATION AMONG KNEE OSTEOARTHRITIS PATIENTS: A proposal to determine the antioxidant content of local melon waste and its potential therapeutic use for osteoarthritis.
THE RELATIONSHIP OF FRUIT INTAKE WITH BRAIN DERIVED NEUROTROPHIC FACTOR (BDNF) PROTEIN LEVEL AMONG ADOLESCENTS IN TERENGGANU: An investigation into the correlation between fruit consumption and BDNF protein levels in adolescents.
STUDY PROPOSAL: PREVALENCE AND RISK FACTORS OF HYPERTENSION AMONG ADULTS IN TERENGGANU’S FELDA: An outline for a study regarding hypertension prevalence and lifestyle factors in rural Felda communities.
THE CONCEPT OF WELL-BEING AMONG HEMODIALYSIS CHRONIC KIDNEY PATIENTS: A CORRELATION STUDY: This study explores the relationship between well-being sub-elements and the quality of life in hemodialysis patients.
STUDY PROPOSAL: DEVELOPMENT OF DIETARY RISK CALCULATOR TO PREDICT CORONARY: A proposal for developing a new dietary risk calculation tool to assess coronary heart disease risk.
Keywords
Life Science, Antibacterial Activity, Trigona Honey, Genetic Polymorphism, Obesity, Molecular Biology, Clinical Research, Public Health, Dietary Fibre, Cardiovascular Disease, Brain Tumor, Probiotics, Thalassemia, Human Sustainability, Cognitive Recovery.
Frequently Asked Questions
What is the core focus of this publication?
This work compiles various research studies and proposals from the Faculty of Health Sciences at UniSZA, primarily focusing on life science experiments, clinical trials, and public health issues pertinent to Malaysia.
What are the central themes discussed in these studies?
The themes include antimicrobial and antibiofilm investigations, genetic associations with metabolic disorders, nutritional interventions for chronic diseases, and studies on mental health and social well-being.
What is the primary objective of the research presented?
The primary goal is to address human sustainability and health by exploring natural remedies, understanding the genetic basis of diseases, and evaluating clinical and lifestyle interventions for better management of chronic conditions.
What scientific methods are utilized in this research?
The researchers employ diverse methodologies including PCR, gene expression profiling, cross-sectional and case-control studies, meta-analyses, agar diffusion assays, and psychological assessments via structured questionnaires.
What topics are covered in the main section of the book?
The main sections cover specific experimental studies such as the use of Trigona honey, genetic characterizations, probiotic applications in periodontitis, and clinical trials related to calorie restriction and cognitive recovery.
Which keywords best characterize the work?
The work is characterized by terms such as Life Science, Genetic Polymorphism, Antibacterial Activity, Obesity, Public Health, and Cardiovascular Disease.
How does Trigona honey demonstrate therapeutic potential according to the studies?
Studies show that Trigona honey possesses significant antioxidant, antibacterial, and antibiofilm properties, which can potentially be used as an alternative treatment for enteric and respiratory bacterial infections.
What does the research suggest about SOX genes in brain tumors?
The research suggests that SOX gene expression patterns, specifically SOX10, SOX9, and SOX13, play a role in brain tumorigenesis and could potentially serve as future diagnostic markers.
- Quote paper
- Mohd Hilmi Abu Bakar (Editor), 2019, Healthy and Beauty. Finding of current life science experiment for human sustainability, Munich, GRIN Verlag, https://www.grin.com/document/493733
