Trichoderma Classification System Based on Color Code Texture of Potato Dextrose Agar Solid Using Tensorflow with Prototype

LIST OF APPENDICES

1 Glossary

2 Source code

3 Questioners

4 Respondent photo Documentation

5 User manual

6 Curriculum vitae (Researcher)

LIST OF TABLES

1 Hardware Requirements

2 Software Requirements

3 Budgetary Requirements

4 Respondents of the Study

5 Likert Scale of Functionality

6 Likert Scale of Reliability

7 Testing of the system day 3

8 Testing of the system day 4

9 Testing of the system day 5

10 Confusion matrix

11 Descriptive rating of the Respondents of Remote-control camera

12 Descriptive ratings of Camera that slides along the track

13 Descriptive rating of the controlled camera system

14 Descriptive rating of the healthy Solid Trichoderma Ready to harvest

15 Descriptive rating of Classify healthy and contaminated

16 Descriptive of the system would not create an error

17 Descriptive rating of the system resumes working and restores lost data after failure

18 Descriptive rating of the system did not rely to any other hardware

19 Descriptive ratings of the system would not cause any error and malfunction

LIST OF FIGURES

1 Conceptual Framework of the study

2 Locale map of the study of Davao del sur state college

3 Waterfall Diagram

4 Theoretical Framework of the study

5 The operational Framework of Trichoderma based on color code Texture

6 Flow Chart

7 Remote control to run the motorized camera dolly

8 Camera that slides along the track, ensuring smooth and steady movement

9 Captured image using controlled camera

10 Determine the healthy solid Trichoderma culture ready to harvest

11 Flow chart

12 Pseudocode of healthy solid Trichoderma ready to harvest

13 Classify difference of solid Trichoderma between healthy and contaminated through color recognition

14 Flow chart

15 Pseudocode of Classification between healthy and contaminated

16 Testing manual efficiency

17 Generate software

18 Training loss

19 Training accuracy

20 Model screen

21 Prototype of the desktop application system

CHAPTER I

Introduction

Background of the Study

A color code, also known as a color scheme, is a method of showing details using various colors. The parameters ambient, diffuse, specular, opacity, and color describe a solid color texture. These color-coded textures are used to determine the different colors of Trichoderma. Trichoderma is a free-living fungus that is highly interactive in the root, soil, and foliar habitats and is offered to organic producers as a wide range of Trichoderma-based products Plant diseases can be controlled with biopesticides, which are biological insecticides. Trichoderma species are also important because they stimulate plant development while inhibiting the growth of plant diseases.(Palm, 2016)

Nowadays, Trichoderma species Due to its well-known biological control mechanism, it's been frequently employed in agricultural applications. Due to the usage of this microbial inoculant in Trichoderma-based products, researchers are interested in learning more about the many prospective advantages of Trichoderma. Plant diseases, growth, breakdown, and bioremediation are all discussed. This article will also look at how they produce secondary metabolites in the agroecosystem. According to (Zin and Badaluddin, 2020), these groundbreaking discoveries bring significant benefits to the agriculture industry in terms of environmentally friendly farming practices.

However, the Trichoderma laboratory in charge at Davao del Sur State College uses only their naked eyes to check the difference between good Trichoderma and contaminated based on the color of the solid Trichoderma. The institution’s Trichoderma laboratory employs one person in-charge and one trained agriculturalist as the head of the laboratory. The procedure is to acquire a small amount of solid Trichoderma, also known as the mother culture in the solid Trichoderma. And move it into the solid Trichoderma container Petri plate (PDA). Pre-heat the transfer needle in the alcohol lamp within one minute to transfer the small amount of solid Trichoderma before using it to keep the Solid Trichoderma from being contaminated. After that process, it must put in the petri plate into the laboratory table/shelve or containing area and ensure that the petri plate is not shaken. The growth of Trichoderma inside the petri plate will not be destructed, and not to touch it. After that, set it aside for a week. The in-charge of the laboratory could then harvest the good solid Trichoderma. In classifying harvesting in solid Trichoderma, it adheres to the product's Quality Control level and automate the manual classification process in time for harvest. Using the sense of sight identify solid Trichoderma in each container as a manual operation, will have a lower level of accuracy. It would be difficult to tell whether the solid Trichoderma is safe or not. As a result, the scientist is working on an application that will enable the person to quickly evaluate and identify the exact state of solid Trichoderma in the lab.(3 and Gallen, 2017)

Therefore, the proponent develop the Trichoderma Classification System based on Color Code Texture of Potato Dextrose Agar Solid (PDA). Using TensorFlow, it has a prototype real track to capture an image and system classify to determine the solid Trichoderma. Also, it automates the manual process by taking a picture of solid Trichoderma with the system's camera. On the other hand, the solid Trichoderma classification process is more reliable when it comes to harvesting, which will help to maintain the product's quality control. The camera will shift automatically using a motorized camera dolly. The captured images automatically recognized and display the result on the computer monitor.

Objective of the Study

The overall goal of this research is to create a “Trichoderma Classification System Based on Color Code Texture of Potato Dextrose Agar Solid (PDA) Using TensorFlow.

Specifically, on the following:

1. To develop a Prototype with the following functionality.
1.1 Remote control to run the motorized camera dolly.
1.2 Camerathat slides along the track, ensuring smooth and steady movement.
1.3 Capture images using controlled camera system.

2. To develop a System has the following functionality.
2.1 Determine the healthy Solid Trichoderma culture Ready to harvest.
2.2 Classify difference of solid Trichoderma between good or contaminated through color recognition.

3. To Evaluate Trichoderma Classification System Based on Color Code Texture of Potato Dextrose Agar Solid (PDA) Using TensorFlow with Prototype in terms of:
3.1 Functionality
3.2 Reliability.

Significance of the Study

The study of Trichoderma classification system based on color code texture of Potato Dextrose Agar Solid (PDA) using TensorFlow with a prototype will be beneficial to the following future researcher, quality control, and the laboratory in charge of the Trichoderma laboratory of Davao del Sur State College.

Future Researcher. This project would provide baseline data and a rich source of a related study that they will be conducting in the future, students and teachers alike in terms of Information Technology innovations. Relevant information from this study would serve as a guide for future developers who may get involved in the same project or related program.

Quality Control. This study will help maintain the quality of Trichoderma in the laboratory, and will help not to enter microorganisms in the laboratory and infect others.

Laboratory In-charge. This study will be the basis for Establishing quality standards, designing operations, quality, and troubleshooting processes, assuring staff compliance, certifying instrument performance, scheduling equipment and replacement, servicing, and repair are all ways to improve laboratory equipment performance.

Scope and Limitations of the Study

The scope of Trichoderma classification system based on color code texture of Potato Dextrose Agar Solid (PDA) using Tensor Flow with prototype is accessible only by the desktop/computer. The use of this system is to classify and determine the Trichoderma by the time of harvesting. Only the color is used to organize and define the excellent Trichoderma like white, green, and dark-green. If the system classifies the different color, it will automatically identify as contaminated Trichoderma. The system will be used after five days or six days of planting the solid Trichoderma. The solid Trichoderma is placed in Petri plates by the researcher. A laboratory is used to perform or analyze the research. The researcher is able to catch 2000-3000 images. This project's data was comprised of captured photos. The laboratory in charge, the agriculturist, and the quality control officer are chosen as experts for this project to determine the reliability and functionality of the application. The researcher presents a standardized survey only for the project. This research is being studied to ensure the consistency of solid Trichoderma.

Definition of Terms

In the context of this study, the following terms were defined either theoretically and/or operationally:

Color code Texture. The colors red, green, and blue are represented by hexadecimal triplets, such as '255' red, '0' green, and '0' blue in the color red. And there's no image mapping or procedural texture mapping, so texture definition is just a solid color.

Trichoderma. This one free-living fungus that is highly interacting in root, soil, and foliar settings (Singh et al., 2014) and is accessible to organic producers as a wide range of Trichoderma-based biopesticides for plant disease control.

TensorFlow. Anyone, from start to end, may utilize this open-source machine learning platform. This is where you can find TensorFlow's diverse ecosystem of tools, libraries, and community resources. TensorFlow provides Machine Learning APIs. TensorFlow is also faster than rival Deep Learning frameworks when it comes to compilation.

Program. This refers to a set of operations that is made to function for a specific task.

Prototype. It's utilized to put a fresh design to the test so that analysts and system users can be more accurate. It is the stage that occurs between the formalization and evaluation of a notion.

Functionality. The number of processes that a computer or other electronic system can perform.

Reliability. One of the most important aspects of a successful psychological evaluation. After all, it wouldn't be very useful if a test was inconsistent and produced different findings every time.

CHAPTER II

Review of Related Study

Related Literatures

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A computer vision gadget that may be used to propose automated high-speed fruit sorting. The fruit region was initially separated from the picture using an Otha-color-space dependent thresholding method; the blob algorithm was used to remove noises in the image; and the spline-interpolation based approach was used to identify fruit contour. The classification function in the fruit sorting process was chosen based on the color ratio of the fruit, which was obtained using the HSI color space. Fruit sorting was handled by the standard Bayes classifier, whose parameters were acquired using a research module. This approach was used to check crystal Fuji apples, and an average sorting accuracy of 90% was attained. Monochrome or greyscale pictures were used in the majority of primary systems. According to(Guo and Cao, 2004), color image processing systems have mostly been utilized in grading to assess fruit quality rather than to detect bruises. This study describes a color image processing-based vision system for sorting Fuji apples. The image-processing method first isolates the apple region from the image's backdrop, then calculates its color ratio, which is the most important factor in determining the quality of Fuji apples. A specific research module is introduced in the software, which is utilized to initialize the parameters of the traditional Bayes classifier responsible for apple classification.

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Several fruit identification methods have been developed based on color and form parameters. Colors and forms of diverse representations of objects, on the other hand, might be similar or identical. As a result, relying just on color and form features for fruit identification is insufficient. In light of this, the researcher offers in this study a technique for detecting things from pictures that employs color, form, and texture. They conducted many trials using fruit photos and determined that the gadget can virtually properly recognize fruit kinds from images. Fruit detection is a technique for identifying occurrences of fruits in photographs. The goal of fruit detection is to find all fruit representations. While there are only a few instances of the things in the photographs, their locations and sizes are vastly different. As a result, capturing fruit positions is a critical aspect of fruit identification from photos (Hossen et al., 2017).

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