The interaction between transcriptional factors and cis-regulatory DNA element has given rise to the diversity in the expression of eukaryotic gene. Prdm15 gene is known to be expressed in the brain throughout development of mouse and zebrafish however; there are no known enhancer(s) for this gene in the brain. The aim of this study was to identify brain specific enhancer(s) for the gene Prdm15 during brain development. The VISTA enhancer browser and Encyclopedia of DNA Elements (ENCODE) data were used in this study to determine our candidate brain enhancer. The VISTA enhancer browser was used to quest for cis-regulatory element(s) in the environs of Prdm15 by comparative genomics and only one candidate enhancer we designated Prdm15 control element 1(PCE1) was identified downstream of the gene. PCE1 was found to be conserved in organisms such as chicken, zebrafish, rhesus, chimp, dog and cow. To ascertain if PCE1 is a brain enhancer, we delineated PCE1 using brain-specific enhancer chromatin modification signatures H3K4me1 and H3K27ac in the ENCODE data and they showed enrichment for PCE1. In conclusion, our data set revealed that only one brain candidate enhancer (PCE1) exist for Prdm15 and that this brain enhancer for Prdm15 will help in identifying important upstream specific transcription factors of PrdmI5 during brain development.
Inhaltsverzeichnis (Table of Contents)
- CHAPTER ONE
- INTRODUCTION
- CHAPTER TWO
- LITERATURE REVIEW
- Prdm gene family.
- Cis-regulatory architecture of the genome.
- Transcriptional regulation by enhancers
- Transcriptional enhancers
- Deregulation of enhancer function in human disease
- Chromatin signatures in different enhancer states
- Interaction between transcription factors and chromatin ..
- Genome wide identification of enhancer element..
- LITERATURE REVIEW
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This dissertation investigates the cis-regulatory network underlying the Prdm15 gene during brain development, aiming to identify brain-specific enhancers for this gene. The study utilizes the VISTA enhancer browser and ENCODE data to identify and characterize these enhancers.
- Cis-regulatory elements and their role in gene expression
- The Prdm15 gene and its function in brain development
- Identification and characterization of brain-specific enhancers for Prdm15
- Chromatin modifications associated with enhancer activity
- The potential role of transcription factors in regulating Prdm15 expression
Zusammenfassung der Kapitel (Chapter Summaries)
Chapter One provides an introduction to the research topic, highlighting the importance of understanding the regulatory mechanisms governing gene expression in brain development. Chapter Two delves into the existing literature, reviewing key concepts such as the Prdm gene family, cis-regulatory elements, enhancer function, chromatin modifications, and methods for identifying enhancers. The chapter discusses the significance of understanding enhancer function in both normal development and disease contexts.
Schlüsselwörter (Keywords)
The key themes and concepts explored in this dissertation include cis-regulatory elements, enhancers, Prdm15, brain development, chromatin modifications, transcription factors, and gene regulation. The study specifically focuses on identifying brain-specific enhancers for the Prdm15 gene, contributing to our understanding of the intricate regulatory networks that govern gene expression during this crucial developmental process.
- Citation du texte
- Michael Adu (Auteur), 2018, Cis-regulatory elements underlying Prdm15 gene during brain development, Munich, GRIN Verlag, https://www.grin.com/document/1169011
