In the present study, effect of agitation rate on growth yield and virulence property of Bordetella pertussis (BP) strain 509 during pilot scale cultivation was investigated. The results indicated significant decrease in growth yield on increasing the agitation rate due to shear stress of bacterial cells. To characterize the shear stress on bacterial cell protein FHA, cell lines such as Vero cells and CHO cells were used to check the adherence property. Finally the virulence property of vaccine culture was screened by Mouse Weight Gain Test (MWGT) and lymphocytosis promoting factor (LPF) test using LACA strains of laboratory mice weighing 14-16 grams. Comparatively more toxicity was observed on higher agitation rates than in optimal and lower agitation. It is evident that higher agitation causes shearing stress in cells inducing more toxicity than optimal agitation which in turn affects the quality of final vaccine.
Inhaltsverzeichnis (Table of Contents)
- Abstract
- Keywords
- Introduction
- Material and Methods
- Preparation of Media
- Opacity Test
- Sterility Test
- Effect of Agitation
- Mouse Weight Gain Test
- LPF Activity
- Filamentous Haemagglutinin (FHA) Adherence Activity towards Vero & CHO Cell Line
- Results and Discussion
- Conclusion
- Reference
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This research article investigates the impact of agitation rate on the growth yield and virulence of Bordetella pertussis strain 509 during pilot scale cultivation for whooping cough vaccine preparation. The study aims to determine the optimal agitation rate for efficient bacterial growth while maintaining the desired virulence properties and minimizing toxicity.
- Effect of agitation rate on growth yield and virulence of Bordetella pertussis
- Shear stress on bacterial cells and its impact on virulence factors
- Assessment of toxicity through mouse weight gain and lymphocytosis promoting factor tests
- Evaluation of the adherence activity of filamentous hemagglutinin (FHA) to Vero and CHO cell lines
- Correlation between agitation rate, cell lysis, and vaccine quality
Zusammenfassung der Kapitel (Chapter Summaries)
The introduction provides background information on Bordetella pertussis, the causative agent of whooping cough, and the importance of pertussis vaccines. It also outlines the various toxins and virulence factors produced by the bacterium.
The materials and methods section details the experimental procedures employed, including the preparation of culture media, the opacity test, the sterility test, and the methods used to assess the effect of agitation on bacterial growth, virulence, and adherence. The mouse weight gain test, lymphocytosis promoting factor (LPF) activity, and FHA adherence assays are also explained.
The results and discussion section presents the findings from the experiments, analyzing the impact of agitation rate on bacterial growth, toxicity, and FHA adherence. The data is presented in tables and figures, and the significance of the findings is discussed in relation to previous research.
Schlüsselwörter (Keywords)
The main keywords and focus topics of the text are: agitation rate, Bordetella pertussis, endotoxin, toxicity, virulence, FHA, cell lysis, vaccine production, whooping cough, pertussis, growth yield, LPF activity, Vero cells, CHO cells, bioreactor, shear stress.
- Citation du texte
- Vidhya Charan (Auteur), 2011, Effect of Agitation Rate on Growth Yield and Virulence of Bordetella pertussis Strain 509 in Lab Scale Cultivation , Munich, GRIN Verlag, https://www.grin.com/document/176400
