Lymphatic filariasis (LF) is a disease that is currently the target of a major global initiative for elimination.During the past decade, both the treatment and the control strategies for LF have undergone major paradigm shifts–due to rapid increase in knowledge and understanding of LF that is derived directly from a series of commendable progress made by scientific and medical research communities.In pursuit of understanding LF a disease to which a social stigma is attached, the chronic pathology (CP) of LF was taken as a model to understand the immunopathological mechanisms that lead to acute lymphangitis and lymphadenitis,in filarial infections, harboring Wuchereria bancrofti.It is a well established fact that elephantiasis is a consequence of immune-reactivity to adult worm antigens. Therefore,it was thought that T-cells infiltrating the lesions in chronic-pathology disease could augment for elevated inflammation seen in CP.An attempt was made to examine T cells by T Cell Receptor Vß analysis using parasite (BmA) and non parasite (PPD) antigen stimulated PBMC’s for RT PCR and agarose gel electrophoresis followed by Integrated Density Values(IDV),analysis which were further validated by Statistical analysis using One-Way ANOVA followed by Tukeys-HSD Test.Therefore,primers designed for specific 24 Vß gene families in the given repertoire were the choice for experimental analysis by PCR. Five CP cases and 5 Endemic Normals who were normal healthy individuals’ considered as controls,and also 2 Microfilaraemics, who are carriers of this disease were inducted into the current study. Specific T-Cell Receptors-TCRVβ1,TCRVβ2,TCRVβ7,TCRVβ14,TCRVβ20, TCRVβ24 which got overrepresented in the CP subjects when the PBMCs of these subjects came in contact with crude antigen of the parasite BmA in an In-Vitro culture system, but MF’s, do not show any overrepresentation for BmA under similar conditions. Similarly TCRVβ5.2 and TCRVβ11 are genes which were preferentially expressed out of TCRBV1-24 gene repertoire in EN upon stimulation with BmA. PPD seems to be a potential stimulator for MF’s. TCRBV21 of CP subjects was expressed high upon PPD stimulation along with EN in which batteries of receptors are expressed which does not carry any statistical significance.PHA stimulated and an unstimulated culture of PBMCs does not show any statistical significant difference in overrepresentation of TCRVβ1-24 genes with respect to EN and MF but PHA did showed significance with CP.
Inhaltsverzeichnis (Table of Contents)
- Abstract
- Acknowledgement
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This thesis aims to investigate the immunopathological mechanisms underlying chronic pathology (CP) in lymphatic filariasis (LF) caused by Wuchereria bancrofti infection. The study focuses on identifying parasite-specific antigens and antibodies in lymphatic fluid from CP patients and comparing them to serum samples from the same patients. It also explores T-cell receptor (TCR) Vβ gene expression in peripheral blood mononuclear cells (PBMCs) stimulated with parasite antigens.
- Identification of parasite-specific antigens and antibodies in lymphatic fluid and serum of CP patients.
- Analysis of TCR Vβ gene expression in PBMCs of CP patients in response to parasite antigens.
- Investigation of the effect of lymphatic fluid on bacterial growth.
- Comparison of immune responses in CP patients, normal healthy individuals (EN), and microfilaria carriers (MF).
- Understanding the role of immune reactivity in the development of elephantiasis.
Zusammenfassung der Kapitel (Chapter Summaries)
The abstract provides an overview of lymphatic filariasis, the study's objectives, and a summary of the key findings. The acknowledgement section expresses gratitude to individuals and institutions that supported the research.
Schlüsselwörter (Keywords)
Lymphatic filariasis, Wuchereria bancrofti, chronic pathology, lymphatic fluid, serum, antigens, antibodies, T-cell receptor (TCR), Vβ gene expression, PBMCs, immunopathology, elephantiasis, bacterial infections, SDS-PAGE, Western blot, RT-PCR.
- Citation du texte
- Dr. Magapu Solomon Sudhakar (Auteur), 2006, The Lymphatic Fluid and T-Cell Receptor Vβ expression in Peripheral Blood Mononuclear Cells (PBMCs) of patients with Wuchereria Bancrofti Infection, Munich, GRIN Verlag, https://www.grin.com/document/183781
