Platelet counts and its associated indices of MPV, PDW and P‐LCR were assayed in a total sample of 98 blood donors aged 19 to 55years here in Porto Novo. Among whom 47 were men(47.96%) and 51 were women (52.04%). The mean platelets count of 218.90 × 109/L were recorded among men samples while Platelet count of 247.8 × 109/L were recorded
among women blood donors. The platelet count were slightly higher in women than men and this increase is not statistically significant (P=0.01). The indices of platelets, MPV, PDW and P‐LCR were also variable among both men and women, again these are not
statistically significant (P=0.01).
Conclusion: The present study showed that platelet count are gender dependent and platelet indices should be paid attention to when diagnosing thrombocytopenia. The routine hematologic autoanalyzers should incorporate reticulated platelet absolutely necessary for the accurate assessment of marrow response to thrombocytopenia.
Table of Contents
1. Aims and Objectives
2. Acknowledgement
3. Abstract
4. Introduction
5. Methodology
6. Result
7. Discussion
8. References
Research Objectives and Focus
This study investigates the profiles of platelet count, Mean Platelet Volume (MPV), Platelet Distribution Width (PDW), and Platelet Large Cell Ratio (P-LCR) in healthy blood donors to evaluate their efficacy as diagnostic indices for thrombocytopenia while specifically examining potential differences between sexes.
- Analysis of platelet count and associated indices (MPV, PDW, P-LCR).
- Evaluation of sex-based differences in hematological profiles.
- Assessment of diagnostic utility for thrombocytopenia.
- Examination of technical factors influencing blood count accuracy.
- Comparative analysis of physiological data in healthy adult donors.
Excerpt from the Book
Platelet Size Calibration
Most instrument measure cells falling between 2 to 20 femtolitres as platelets. This raw data is plotted on the platelet histogram. While the bulk of the platelet population falls within a fixed threshold, others exist outside these boundaries, microthrombocytes on the low end and macrothrombocytes on the high end. However, cytoplasmic fragment, Microcytic red cells and debris can be included as platelets. To overcome this problem, mathematical curve fitting is applied to the raw data histogram to deliver a more reliable platelet count, therefore eliminating non- platelet particles. The raw data (2-20FL) is tested against a set of criteria and fitted to a log normal curve from 0 to 70 FL. The curve must be positive, the mode must fall between 3 and 15 FL and the coefficient of variation of platelet size or PDW is less than 20%. Once these criteria are met, the autoanalyzer uses the fitted curve to determine the platelet count (equal to area under the curve from 0 to 70 FL).
Summary of Chapters
Aims and Objectives: Defines the research intent to assess platelet indices as diagnostic tools for thrombocytopenia and explores gender-related variations.
Acknowledgement: Expresses gratitude to the author's family and the academic staff at Atlantic International University for their guidance.
Abstract: Summarizes the study of 98 blood donors, noting higher platelet counts in women and the necessity of incorporating reticulated platelet assessment in clinical diagnostics.
Introduction: Provides a comprehensive overview of platelet biology, megakaryocyte progenitor stages, and the hormonal regulation of megakaryocytopoiesis.
Methodology: Details the sampling process of 98 donors in Porto Novo and the technical procedures for automated platelet counting and calibration.
Result: Presents categorized data on platelet count, MPV, PDW, and P-LCR for men and women across different age groups.
Discussion: Interprets the findings regarding gender differences and technical challenges in measuring platelet indices, highlighting the need for accurate diagnostic standards.
References: Lists the academic literature and scientific studies supporting the analysis of platelet function and diagnostic parameters.
Keywords
Platelet count, MPV, PDW, P-LCR, Thrombocytopenia, Megakaryocytes, Hematology, Blood donors, Gender differences, Automated counting, Platelet indices, Clinical diagnosis, Endomitosis, Thrombopoiesis, Cytokines.
Frequently Asked Questions
What is the core focus of this research?
The research focuses on investigating platelet count and associated indices like MPV, PDW, and P-LCR, and evaluating whether these markers show significant differences between men and women.
What are the primary thematic fields covered?
The work covers platelet biology, megakaryocytopoiesis, the technical aspects of automated blood analysis, and clinical diagnostic interpretation of platelet indices.
What is the main research objective?
The study aims to determine the diagnostic utility of platelet profiles for thrombocytopenia and to confirm if sex-based differences exist in healthy adult populations.
Which scientific methods were employed?
The study utilized blood samples from 98 donors, analyzed via sysmex KX21-N autoanalyzer, and applied ANOVA tests to compare variations between male and female test groups.
What topics are discussed in the main body?
The main body covers the physiological structure of platelets, the life cycle of megakaryocytes, factors causing erroneous platelet counts, and the importance of precise calibration in laboratory settings.
Which keywords characterize this paper?
The paper is characterized by terms such as platelet count, MPV, PDW, P-LCR, thrombocytopenia, and megakaryocyte development.
How does the study address the influence of blood collection on results?
The author discusses how methods like capillary finger pricks versus venous punctures, as well as the use of anticoagulants like EDTA, can lead to discrepancies or falsely low/high platelet counts.
What conclusion does the author reach regarding diagnostic practices?
The author concludes that platelet counts are gender-dependent and recommends that routine hematologic autoanalyzers incorporate reticulated platelet measurement for more accurate assessment of marrow response.
- Citation du texte
- Dr. Peter Ubah Okeke (Auteur), 2012, Platelet Count and Indices of Mean Platelet Volume, Platelet Distribution Width and Platelet Large Cell Ratio - Is there any Sex Difference?, Munich, GRIN Verlag, https://www.grin.com/document/191552
