This study reports the extracellular synthesis of silver nanoparticles by Rhizopus stolonifer and its efficacy against multidrug resistant (MDR) E.coli and S.aureus isolated from Khwaja Bande Nawas Hospital, Gulbarga, Karnataka. Synthesis of silver nanoparticles (AgNPs) was carried out by using fungal filtrate of R.stolonifer and an aqueous solution of AgNO3. The characterization of AgNPs was made by UV-Visible absorption Spectroscope, Scanning Electron Microscope and Energy Dispersed Spectroscope (SEM-EDS), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy and Atomic Force Microscope (AFM). TEM micrograph revealed the formation of spherical nanoparticles with size ranging between 3 to 20 nm. Atomic force microscopy gives the three dimensional structure of the particles. The presence of proteins was detected by FTIR spectroscopy. Three dimensional structure of AgNPs was studied by AFM. AgNPs produced by R.stolonifer gave good antibacterial activity against clinical isolates which were multidrug resistant. Here we report the efficacy of mycogenic metal nanosilver against MDR strains which is difficult through conventional chemotherapy.
Inhaltsverzeichnis (Table of Contents)
- Introduction
- Materials and Methods
- Biosynthesis of silver nanoparticles
- Characterization of silver nanoparticles
- Source of microorganisms
- Analysis of the Antibacterial activity of silver nanoparticles
- Result
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This study investigates the synthesis of silver nanoparticles using the fungus Rhizopus stolonifer and explores its antibacterial activity against multidrug resistant (MDR) strains of E. coli and S. aureus.- Biosynthesis of silver nanoparticles using R. stolonifer
- Characterization of the synthesized nanoparticles
- Antibacterial activity of the nanoparticles against MDR strains
- Potential applications of mycogenic metal nanosilver against MDR strains
- Comparison of the efficacy of nanosilver against conventional chemotherapy
Zusammenfassung der Kapitel (Chapter Summaries)
- Introduction: This section introduces the concept of nanotechnology, highlighting the importance of developing eco-friendly methods for nanoparticle synthesis. It addresses the challenge posed by multidrug resistant bacteria and the potential of silver nanoparticles as a solution.
- Materials and Methods: This chapter outlines the experimental procedures for biosynthesis, characterization, and antibacterial activity evaluation of silver nanoparticles. It details the specific fungal strain used (R. stolonifer), the growth conditions, and the methods employed for characterizing the nanoparticles.
- Result: This section presents the results of the study, demonstrating the successful synthesis of silver nanoparticles by R. stolonifer. It includes information on the characterization of the nanoparticles using various techniques, such as UV-Vis spectroscopy, SEM-EDS, TEM, FTIR, and AFM. Additionally, it reports the antibacterial activity of the synthesized nanoparticles against MDR strains of E. coli and S. aureus.
Schlüsselwörter (Keywords)
This study focuses on the synthesis and antibacterial activity of silver nanoparticles produced using a fungal-based approach. Key concepts include R. stolonifer, silver nanoparticles, multidrug resistant strains, and mycogenic metal nanosilver.Frequently Asked Questions
How are silver nanoparticles (AgNPs) synthesized in this study?
The AgNPs are synthesized extracellularly using the fungal filtrate of Rhizopus stolonifer combined with an aqueous solution of silver nitrate (AgNO3).
What is the significance of using Rhizopus stolonifer?
Rhizopus stolonifer serves as an eco-friendly biological agent for the green synthesis of nanoparticles, offering a sustainable alternative to chemical methods.
What are the characteristics of the synthesized nanoparticles?
The nanoparticles are spherical in shape, ranging in size from 3 to 20 nm, as confirmed by Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).
Against which bacteria are these nanoparticles effective?
They show strong antibacterial activity against multidrug resistant (MDR) strains of Escherichia coli and Staphylococcus aureus.
Why is mycogenic nanosilver a potential solution for MDR strains?
Conventional chemotherapy often fails against MDR strains; however, mycogenic metal nanosilver provides a novel mechanism to combat these difficult-to-treat infections.
Which techniques were used to characterization the AgNPs?
The study utilized UV-Visible spectroscopy, SEM-EDS, TEM, FTIR, and AFM to analyze the structure, size, and composition of the particles.
- Quote paper
- Afreen Banu (Author), Vandana Rathod (Author), E. Ranganath (Author), 2011, Synthesis and characterization of silver nanoparticles by "Rhizopus stolonifer" and its activity against multidrug resistant "Escherichia coli" and "Staphylococcus aureus", Munich, GRIN Verlag, https://www.grin.com/document/192147
