This study reports the extracellular synthesis of silver nanoparticles by Rhizopus stolonifer and its efficacy against multidrug resistant (MDR) E.coli and S.aureus isolated from Khwaja Bande Nawas Hospital, Gulbarga, Karnataka. Synthesis of silver nanoparticles (AgNPs) was carried out by using fungal filtrate of R.stolonifer and an aqueous solution of AgNO3. The characterization of AgNPs was made by UV-Visible absorption Spectroscope, Scanning Electron Microscope and Energy Dispersed Spectroscope (SEM-EDS), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy and Atomic Force Microscope (AFM). TEM micrograph revealed the formation of spherical nanoparticles with size ranging between 3 to 20 nm. Atomic force microscopy gives the three dimensional structure of the particles. The presence of proteins was detected by FTIR spectroscopy. Three dimensional structure of AgNPs was studied by AFM. AgNPs produced by R.stolonifer gave good antibacterial activity against clinical isolates which were multidrug resistant. Here we report the efficacy of mycogenic metal nanosilver against MDR strains which is difficult through conventional chemotherapy.
Inhaltsverzeichnis (Table of Contents)
- Introduction
- Materials and Methods
- Biosynthesis of silver nanoparticles
- Characterization of silver nanoparticles
- Source of microorganisms
- Analysis of the Antibacterial activity of silver nanoparticles
- Result
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This study investigates the synthesis of silver nanoparticles using the fungus Rhizopus stolonifer and explores its antibacterial activity against multidrug resistant (MDR) strains of E. coli and S. aureus.- Biosynthesis of silver nanoparticles using R. stolonifer
- Characterization of the synthesized nanoparticles
- Antibacterial activity of the nanoparticles against MDR strains
- Potential applications of mycogenic metal nanosilver against MDR strains
- Comparison of the efficacy of nanosilver against conventional chemotherapy
Zusammenfassung der Kapitel (Chapter Summaries)
- Introduction: This section introduces the concept of nanotechnology, highlighting the importance of developing eco-friendly methods for nanoparticle synthesis. It addresses the challenge posed by multidrug resistant bacteria and the potential of silver nanoparticles as a solution.
- Materials and Methods: This chapter outlines the experimental procedures for biosynthesis, characterization, and antibacterial activity evaluation of silver nanoparticles. It details the specific fungal strain used (R. stolonifer), the growth conditions, and the methods employed for characterizing the nanoparticles.
- Result: This section presents the results of the study, demonstrating the successful synthesis of silver nanoparticles by R. stolonifer. It includes information on the characterization of the nanoparticles using various techniques, such as UV-Vis spectroscopy, SEM-EDS, TEM, FTIR, and AFM. Additionally, it reports the antibacterial activity of the synthesized nanoparticles against MDR strains of E. coli and S. aureus.
Schlüsselwörter (Keywords)
This study focuses on the synthesis and antibacterial activity of silver nanoparticles produced using a fungal-based approach. Key concepts include R. stolonifer, silver nanoparticles, multidrug resistant strains, and mycogenic metal nanosilver.- Citation du texte
- Afreen Banu (Auteur), Vandana Rathod (Auteur), E. Ranganath (Auteur), 2011, Synthesis and characterization of silver nanoparticles by "Rhizopus stolonifer" and its activity against multidrug resistant "Escherichia coli" and "Staphylococcus aureus", Munich, GRIN Verlag, https://www.grin.com/document/192147
