Synthesis of New Magnetic Nanoparticles and Study their Effect on Prolactin Structure

Thesis (M.A.) , 2014 , 100 Pages

Chemistry - Bio-chemistry

The National Nanotechnology Initiative defines nanotechnology as the manipulation of matter to at least one dimension sized from 1 to 100 nm. This definition shifted from a particular technological goal to a research category inclusive of all types of research and technologies that deal with the special properties of matter that occur below the given size threshold. These unique effects often provide nanoscale materials the desired chemical, physical, and biological properties that differ from those of their larger or bulk counterparts .

Table of Contents

Chapter One

Introduction

I-1Nanotechnology

I-2 Nanoparticle

I-3 Properties

I-3-A-Shape

I-3-B- Physical properties

I-3-C-Chemical Properties

I-3-D- Optical properties

I-3-E- Ferromagnetic Properties

I-4 Surface coating for biological applications

I-5 Applications of NPs in biology and medicine

I-6 Magnetic nanoparticles

I-6 A-Definition

I-6 B-Properties

I-6 C-Types of magnetic nanoparticles

1-Oxides: ferrite1

2- Metallic

3- Metallic with a shell

Preparation Methods of Magnetic

1-Co-precipitation

2- Thermal decomposition

3- Microemulsion

4- Flame spray synthesis

I -7Applications Of Magnetic NPs

A) Medical diagnostics and treatments

B) Magnetic immunoassay

C) Waste water treatment

D) Chemistry

E) Biomedical imaging

F) Information storage

G) Genetic engineering

H) Industrial applications

I) Biomedical applications

J) In vivo applications

K) Removal of organic pollutants

L) Removal of Inorganic pollutants

M) Analytical applications

I-8-Preperation of the modified magnetic NPs

I-9 Identification of NP

I-9-1 Scanning electron microscope (SEM)

I-9-2 Transmission electron microscopy (TEM)

I-9-3 Wide angle x-ray scattering (WAXS) or wide-angle X-ray diffraction (WAXD)

I-9-4- FTIR

I-9-5- Dynamic Light Scattering

I-9-6-Zeta potential analysis

I-9-7 Magnetic property (magnetic behavior)

I-10- Protein-NPs Interaction

I-10- 1-Covalent Protein-NP Conjugation

I-10- 2-Non-Covalent Protein-NP Conjugation

I-10-3 Application of protein-NPs conjugates.

I-11- Prolactin (PRL)

I-12 Folic acid

I-13- Palmitic acid

I-14- Adsorption of Proteins

I -11-Adsorption isotherms

a-Ionic or Electrostatic Interactions

b-Hydrogen Bonding

c-Hydrophobic Interactions

d-Charge-Transfer Interactions

Aim of the study

Chapter two

II- Method And Materials

II-1 Chemicals

II-2 Instruments

II-3-Methods

II-3-1- Synthesis of the Fe3O4nanoparticales

II-3-2 Synthesis of magnetic nanoparticales-palmatic acid

II-3-3 Synthesis of magnetic nanoparticales-folic acid

II-3-4 Preparation of Phosphate Buffer Saline (pH=7.4)

II-4 Identification of the prepared NPs

II-4-1 Identification of the Prepared MNPs by TEM

II-4-2 Identification of the Prepared MNPs by SEM

II-4-3 FTIR charts

II-4-4 Dynamic light scattering (DLS) method

II-4-5 TEM study for MNPs- Prolactincomplexes

II-4-6 TGA Characters.

II-5 Estimation of Prolactine Concentration by ELISA

A-Principle:

B- Reagents of the kit

C-Reagent Preperation

D-Test Procedure

II-6 Estimation of Equilibrium Time of Adsorption

II-7 Adsorption Isotherms

II-8 Thermodynamicsof the Adsorption of PRL on MNPs

II-9 Desorption process

Chapter three

Result & discussion

III.1 Characterization of the synthesized NP.

III.1.1 MNPs

III.1.2 MNP@Folic

III.1.2 MNP@Palmitic

III-2 Prolactin-MNP compounds Interaction

III-2-1-Equilibrium Time

III-2-2-Properties of NP after interaction with prolactin hormone

III-2-3 Adsorption Process

III-2-4 Applicability of Langmuir and Freundlisch Adsorption Isotherms in the NP-Prolactin systems.

III-2-5 Thermodynamics of the adsorption process:

III-2-6 Desorption Processes:

Application of protein-NPs conjugates.

Conclusions

Recommendations

References

Research Objectives and Thematic Focus

The primary research objective of this work is the development and synthesis of novel magnetic nanoparticles (MNPs) through the surface modification of standard magnetic nanoparticles. The study aims to utilize these functionalized nanoparticles as substrates for the immobilization of the prolactin hormone, investigating the interaction kinetics and thermodynamic properties to evaluate their potential for future medical and diagnostic applications.

• Synthesis and surface functionalization of magnetic nanoparticles (MNPs) with folic and palmitic acid.
• Characterization of synthesized nanoparticles using electron microscopy (TEM, SEM), FTIR, and thermal analysis (TGA).
• Analysis of the adsorption and immobilization mechanisms of prolactin hormone onto the modified MNP surfaces.
• Evaluation of thermodynamic parameters (ΔG, ΔH, ΔS) and desorption kinetics to assess surface-protein interaction stability.

Book Excerpt

1-1 Nanotechnology:

The National Nanotechnology Initiative defines nanotechnology as the manipulation of matter to at least one dimension sized from 1 to 100 nm (Timothy, 2011). This definition shifted from a particular technological goal to a research category inclusive of all types of research and technologies that deal with the special properties of matter that occur below the given size threshold (Lydia et al., 2014). These unique effects often provide nanoscale materials the desired chemical, physical, and biological properties that differ from those of their larger or bulk counterparts (Ye et al., 2011)

Nanotechnology allows scientists, engineers, chemists, and physicians to work at the molecular and cellular levels to produce important advances in life sciences and healthcare. Defined by size, nanotechnology has very broad application, including fields of science as diverse as surface science, organic and inorganic chemistry, molecular biology, semiconductor physics, and microfabrication (Saini et al., 2010)

It is one of the most important research and development frontiers in modern science, and now widely used throughout pharmaceutical, medicine, electronics, robotics, and tissue engineering industries. The use of nanoparticle (NP) materials offers many advantages because of their unique size and physical properties (Freitas, 2005; Faraji et al., 2010).

Summary of Chapters

Chapter One: Provides an extensive theoretical background on nanotechnology, magnetic nanoparticles, and the fundamental principles of protein-nanoparticle interactions.

Chapter two: Details the experimental methodologies, including the specific chemicals and analytical instrumentation used to synthesize and characterize the modified magnetic nanoparticles.

Chapter three: Presents the experimental findings regarding the characterization of synthesized particles and the detailed results of the adsorption and desorption interactions between prolactin and the modified MNP surfaces.

Keywords

Magnetic Nanoparticles, Prolactin, Surface Modification, Adsorption Isotherms, Folic Acid, Palmitic Acid, Protein Immobilization, Nanotechnology, Thermodynamics, Desorption, Langmuir, Freundlich, Biocompatibility, Drug Delivery, Characterization

Frequently Asked Questions

What is the core focus of this research?

The research focuses on the synthesis of new magnetic nanoparticles modified with organic molecules and their subsequent application in immobilizing the prolactin hormone on their surfaces.

What are the primary fields of study involved?

The work integrates elements of nanotechnology, surface chemistry, pharmaceutical science, and biochemistry, specifically focusing on protein-material interactions.

What is the ultimate research goal?

The goal is to develop functionalized nanocarriers capable of efficiently binding prolactin, providing a foundation for potential future applications in medical diagnostics and therapy.

Which scientific methods are employed for characterization?

The study uses Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and Thermogravimetric Analysis (TGA) to identify and evaluate the synthesized nanoparticles.

How is the main body structured?

The main body moves from a theoretical literature review to the experimental procedures in the second chapter, and concludes with the presentation and discussion of experimental results, including adsorption isotherms and thermodynamic modeling.

What are the key descriptors for this work?

Key concepts include Magnetic Nanoparticles, Prolactin, Surface Modification, Adsorption Isotherms, and Protein-NP Conjugation.

How do the researchers define the adsorption behavior?

The researchers model the adsorption behavior using the Langmuir and Freundlich isotherm equations to determine the homogeneity and surface forces involved in the interaction between the hormone and the nanoparticles.

How does temperature affect the adsorption process?

The study investigates adsorption at 25°C, 35°C, and 45°C, finding that the process is generally exothermic and that temperature variations influence the desorption percentages, depending on the specific surface modification.

Why are Folic and Palmitic acid used?

These organic molecules are used to modify the surface of the magnetic nanoparticles to enhance their stability, change their interfacial properties, and enable the controlled binding of proteins.

• College: University of Kufa (University of Kufa)
• Authors: Hussein Alhakeim (Author), Mea'ad M. Redha (Author), Ashour H. Dawook (Author)
• Publication Year: 2014
• Pages: 100
• Catalog Number: V283116
• ISBN (eBook): 9783656831839
• ISBN (Book): 9783656830948
• Language: English
• Tags: synthesis, magnetic, nanoparticles, study, effect, prolactin, structure