At present, both price and availability of crude oil have motivated a worldwide search for cheap alternatives. Starch is an alternative source of energy because it is both renewable and available throughout the globe in large quantities. There are varieties of products which can be obtained from starch biomass via hydrolysis. Alcohol is one of the largest volumes of products that can be produced from biomass.
Recently there has been an active research aimed at attaining an increase in ethanol productivity by fermentation. A large amount of potatoes is wasted every year due to improper cold storage facilities in developing and underdeveloped countries. So, saccharification and conversion of these huge amounts of excess potatoes into alcohol, especially ethanol may generate an employment potential apart from saving a good amount of foreign exchange.
In my present research work, the production of alcohol from different varieties of potato, showed a better result after enzymatic hydrolysis of those potatoes followed by alcohol(ethanol) production utilizing Saccharomyces cerevisiae NCIM 3314. Maximum alcohol(ethanol) was obtained utilizing high grade Kufri Jyoti variety when compared to other varieties of potatoes.
Inhaltsverzeichnis (Table of Contents)
- INTRODUCTION
- MATERIALS & METHODS
- Raw Material
- Microorganism used
- Conversion of starch to glucose by acid hydrolysis method
- Enzymatic conversion of starch to glucose
- Optimization of temperature for alcohol production
- RESULTS & DISCUSSION
- CONCLUSION
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
The objective of this research was to investigate the fermentative production of alcohol, specifically ethanol, from potato starch using Saccharomyces cerevisiae. The study aimed to determine the optimal conditions for ethanol production, comparing different potato varieties and hydrolysis methods.
- Comparison of acid and enzymatic hydrolysis of potato starch for glucose production.
- Optimization of fermentation conditions (temperature) for maximal ethanol yield.
- Evaluation of different potato varieties for ethanol production efficiency.
- Assessment of the potential of using potato starch as a renewable resource for alcohol production.
- Exploration of the role of Saccharomyces cerevisiae in the fermentation process.
Zusammenfassung der Kapitel (Chapter Summaries)
INTRODUCTION: This chapter introduces the context of the research by highlighting the global search for cheaper alternatives to crude oil. It emphasizes the potential of starch, particularly from potatoes, as a renewable and abundant energy source. The chapter discusses the various products derivable from starch hydrolysis, focusing on alcohol (ethanol) as a major product and the significance of enhancing ethanol productivity through fermentation. The problem of potato waste in developing countries is highlighted, emphasizing the economic benefits of converting this waste into ethanol. The chapter establishes the rationale for using Saccharomyces cerevisiae and different potato varieties in the study.
MATERIALS & METHODS: This chapter details the methodology used in the research. It describes the procurement and preparation of different potato varieties (Kufri Chandramukhi, Kufri Jyoti, Kufri Nainital), the maintenance and preparation of the Saccharomyces cerevisiae NCIM 3314 culture, and the procedures for both acid and enzymatic hydrolysis of potato starch using diastase. The chapter outlines the methods employed for glucose estimation (DNS method) and alcohol estimation (distillation and A.O.A.C methods). Crucially, the experimental setup for fermentation, including temperature control and anaerobic conditions, is thoroughly explained. The optimization of temperature for alcohol production is also described, including the specific temperatures tested (26±2°C, 30±2°C, and 35±2°C).
RESULTS & DISCUSSION: This chapter presents and interprets the experimental results. It compares the glucose production yields from acid hydrolysis and enzymatic hydrolysis, demonstrating the superior efficiency of the latter. The chapter then details the results obtained from using different potato varieties in the enzymatic hydrolysis process, highlighting the highest alcohol yield achieved with the Kufri Jyoti variety. Finally, it presents data on the effect of fermentation temperature on alcohol production, identifying the optimal temperature for maximal yield. The discussion section likely integrates these findings, interpreting the results in relation to the existing literature on ethanol production from starch.
Schlüsselwörter (Keywords)
Potato, starch, diastase, saccharification, Saccharomyces cerevisiae, ethanol, enzymatic hydrolysis, acid hydrolysis, fermentation, biofuel, renewable energy, Kufri Jyoti.
FAQ: Fermentative Production of Alcohol from Potato Starch
What is the main objective of this research?
The research aimed to investigate the fermentative production of ethanol from potato starch using Saccharomyces cerevisiae, determining optimal conditions for ethanol production by comparing different potato varieties and hydrolysis methods.
What methods were used to convert starch to glucose?
Both acid hydrolysis and enzymatic hydrolysis (using diastase) were employed to convert potato starch into glucose. The research compared the efficiency of these two methods.
What potato varieties were used in this study?
The study utilized three different potato varieties: Kufri Chandramukhi, Kufri Jyoti, and Kufri Nainital.
What microorganism was used in the fermentation process?
Saccharomyces cerevisiae NCIM 3314 was used for the fermentation of glucose into ethanol.
How was the fermentation temperature optimized?
The optimal fermentation temperature was determined by testing different temperatures: 26±2°C, 30±2°C, and 35±2°C. The results indicated an optimal temperature for maximal ethanol yield.
Which hydrolysis method proved more efficient?
The results showed that enzymatic hydrolysis using diastase was more efficient in producing glucose from potato starch compared to acid hydrolysis.
Which potato variety yielded the highest alcohol production?
Among the three varieties tested, Kufri Jyoti showed the highest alcohol yield during enzymatic hydrolysis.
What methods were used for glucose and alcohol estimation?
Glucose was estimated using the DNS method, while alcohol was estimated using distillation and A.O.A.C methods.
What are the key themes explored in this research?
Key themes include comparing acid and enzymatic hydrolysis, optimizing fermentation conditions, evaluating different potato varieties, assessing the potential of potato starch as a renewable resource, and exploring the role of Saccharomyces cerevisiae in ethanol production.
What is the broader context or significance of this research?
The research addresses the global need for cheaper alternatives to crude oil, highlighting the potential of potato starch as a renewable and abundant energy source. It also considers the economic benefits of converting potato waste into ethanol, particularly in developing countries.
What are the key words associated with this research?
Key words include Potato, starch, diastase, saccharification, Saccharomyces cerevisiae, ethanol, enzymatic hydrolysis, acid hydrolysis, fermentation, biofuel, renewable energy, Kufri Jyoti.
- Quote paper
- Debajyoti Bose (Author), 2014, Potentials of Potato Starch for Biofuel Production. Fermentative Production of Alcohol by Saccharomyces Cerevisiae utilizing Potato Starch, Munich, GRIN Verlag, https://www.grin.com/document/341834
