At present, both price and availability of crude oil have motivated a worldwide search for cheap alternatives. Starch is an alternative source of energy because it is both renewable and available throughout the globe in large quantities. There are varieties of products which can be obtained from starch biomass via hydrolysis. Alcohol is one of the largest volumes of products that can be produced from biomass.
Recently there has been an active research aimed at attaining an increase in ethanol productivity by fermentation. A large amount of potatoes is wasted every year due to improper cold storage facilities in developing and underdeveloped countries. So, saccharification and conversion of these huge amounts of excess potatoes into alcohol, especially ethanol may generate an employment potential apart from saving a good amount of foreign exchange.
In my present research work, the production of alcohol from different varieties of potato, showed a better result after enzymatic hydrolysis of those potatoes followed by alcohol(ethanol) production utilizing Saccharomyces cerevisiae NCIM 3314. Maximum alcohol(ethanol) was obtained utilizing high grade Kufri Jyoti variety when compared to other varieties of potatoes.
Table of Contents
INTRODUCTION
MATERIALS & METHODS
I. Raw Material
II. Microorganism used
III. Conversion of starch to glucose by acid hydrolysis method
IV. Enzymatic conversion of starch to glucose
V. Optimization of temperature for alcohol production
RESULTS & DISCUSSION
CONCLUSION
Research Objectives and Key Topics
The primary objective of this research is to evaluate the feasibility of producing ethanol from potato starch, addressing the issue of agricultural waste in developing countries while exploring sustainable alternatives to crude oil. The study investigates various hydrolysis techniques and fermentation conditions to maximize alcohol yields using the yeast strain Saccharomyces cerevisiae.
- Comparison of acid hydrolysis versus enzymatic hydrolysis for starch conversion.
- Evaluation of different potato varieties as potential feedstocks for ethanol production.
- Optimization of fermentation temperature for enhanced alcohol yield.
- Characterization of the utilization of potato waste to reduce environmental and economic impacts.
Excerpt from the Publication
INTRODUCTION
The usual sources of raw material for alcohol production from starch are cereals, grains such as corn, wheat, rye, barley, milo (sorghum grains),rice etc. Other types of starch are available from potatoes of all kinds [1].The amount of alcohol produced from potato starch can vary with the variety of potatoes such as Kufri Chandramukhi, Kufri Jyoti, Kufri Nainital etc.
Generally, potato contains 12-17 % of starch. The size of starch granules present in potato tuber cells ranges from 5-100 micrometers. These granules contains little amount of water, organic & mineral substances other than starch. The distribution of starch in potato tuber is uneven. The cells close to vascular system carries more starch than the cells nearer to the cortex. Smallest amount of starch develops in the pith [2-3].
Alcohol like ethanol can be produced from potato starch through fermentation process. Fermentative production of ethanol as an alternative to petroleum is gaining importance due to the realization of diminishing natural oil & gas resources [4]. Apart from the prime alternatives to petrol(gasohol; 80% gasoline + 20% ethanol), ethanol is also used as a solvent & is a versatile feedstock, used to produce various organic chemicals & their derivatives [5].
Summary of Chapters
INTRODUCTION: Provides an overview of ethanol production from starch sources and discusses the rationale for using potato waste as a sustainable alternative feedstock.
MATERIALS & METHODS: Details the experimental procedures including potato preparation, the use of Saccharomyces cerevisiae, and the specific hydrolysis methods employed.
RESULTS & DISCUSSION: Presents experimental findings comparing hydrolysis efficiency and identifying optimal fermentation temperatures and potato varieties for peak ethanol yield.
CONCLUSION: Summarizes the findings, confirming that enzymatic hydrolysis of high-grade potatoes is an effective method for large-scale ethanol production.
Keywords
Potato, starch, diastase, saccharification, Saccharomyces cerevisiae, ethanol, fermentation, hydrolysis, biofuel, renewable energy, biomass, agricultural waste, distillation, yeast, glucose.
Frequently Asked Questions
What is the core focus of this research paper?
The paper explores the fermentative production of ethanol using potato starch, aiming to turn agricultural potato waste into a valuable fuel source.
What are the primary thematic areas covered?
The themes include starch hydrolysis methods, the performance of different potato varieties as raw materials, and the optimization of fermentation parameters for yeast.
What is the ultimate goal of the study?
The goal is to determine the most efficient method for converting starch into fermentable sugars and maximizing alcohol production yield to promote economic and sustainable development.
Which scientific methods were applied in this work?
The study utilizes enzymatic and acid hydrolysis to convert starch to glucose, followed by anaerobic fermentation using Saccharomyces cerevisiae and subsequent distillation.
What does the main body discuss?
It covers the experimental preparation of potato slurries, the specific inoculation and incubation of yeast, and the analytical comparison of glucose conversion rates.
Which keywords define this research?
Key terms include potato, ethanol, fermentation, enzymatic hydrolysis, and Saccharomyces cerevisiae.
How did the author distinguish between hydrolysis methods?
The research compared acid hydrolysis and enzymatic hydrolysis, finding that enzymatic hydrolysis with diastase provided a significantly higher conversion percentage.
Which potato variety showed the best results?
The high-grade Kufri Jyoti variety proved to be the most efficient for alcohol production, yielding 74.23% in the experiments conducted.
- Citar trabajo
- Debajyoti Bose (Autor), 2014, Potentials of Potato Starch for Biofuel Production. Fermentative Production of Alcohol by Saccharomyces Cerevisiae utilizing Potato Starch, Múnich, GRIN Verlag, https://www.grin.com/document/341834
