The Cucurbitaceae family is the one of the economically important group of plants in the tropics and subtropics. Molecular phylogenetic analysis were advanced after the introduction of molecular markers which give much precise results in analysis. Our current study based on the amplification of RuBisco enzyme using rbcL primer and subsequent validation using BLAST, FASTA and CLUSTAL-W in pumpkin and winter melon.
The isolated and purified DNA samples were PCR amplified using rbcL primer and later sequenced using ABI Prism 377 DNA sequencer. Multiple sequence alignment algorithms and distance matrix were constructed using rbcL sequences in FASTA format were retrieved from GenBank.
Phylogenetic tree was created using the distance based neighbour joining (NJ) and clustering algorithms method. Once divergences between all pairs of samples were determined, statistical cluster analysis and dendrogams examines the similarity among halotypes. Bootstrapping and jackknifing further increase the reliability estimates for the position of haplotypes within the evolutionary tree.
Inhaltsverzeichnis (Table of Contents)
- Acknowledgements
- Table of contents
- Table of figures
- Table of tables
- List of abbreviations
- Molecular phylogenetic studies of pumpkin (Cucurbita argyrosperma) and Winter melon (Benincasa hispida): family comparison using rbcL sequence analysis
- Abstract
- Introduction
- Materials and methods
- Plant samples collection
- Analysis of the sequences BLAST and Clustal-W.
- Construction of phylogenetic tree
- Statistical analysis
- Results
- Discussion
- Conclusions
- Acknowledgements
- References
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This study aims to investigate the phylogenetic relationships between pumpkin (Cucurbita argyrosperma) and winter melon (Benincasa hispida) within the Cucurbitaceae family. The research utilizes rbcL sequence analysis to compare the molecular characteristics of these two species and establish their evolutionary connections.
- Phylogenetic analysis of Cucurbita argyrosperma and Benincasa hispida
- Comparative analysis of rbcL sequences within the Cucurbitaceae family
- Evolutionary relationships between pumpkin and winter melon
- Molecular characterization of Cucurbita argyrosperma and Benincasa hispida
- Application of rbcL sequence analysis in phylogenetic studies
Zusammenfassung der Kapitel (Chapter Summaries)
- Abstract: Provides a concise overview of the research objectives, methodology, and key findings. It highlights the use of rbcL sequence analysis to determine the phylogenetic relationships between pumpkin and winter melon.
- Introduction: Introduces the Cucurbitaceae family, emphasizing the importance of phylogenetic studies in understanding plant evolution and diversity. It also provides background information on pumpkin and winter melon, outlining their economic and agricultural significance.
- Materials and methods: Details the methods used in the study, including plant sample collection, DNA extraction, PCR amplification, sequencing, and phylogenetic analysis. This section explains the steps involved in obtaining and analyzing the rbcL sequence data.
- Results: Presents the findings of the study, including the results of the sequence alignment, phylogenetic tree construction, and statistical analysis. This section showcases the key insights gained from the molecular data.
- Discussion: Interprets the results of the study, drawing connections to existing literature and addressing the implications of the findings. It discusses the evolutionary relationships between pumpkin and winter melon, highlighting their position within the Cucurbitaceae family.
Schlüsselwörter (Keywords)
The primary focus of this research lies in the field of molecular phylogenetics, specifically exploring the relationships between pumpkin and winter melon within the Cucurbitaceae family. Key terms include rbcL sequence analysis, phylogenetic tree construction, molecular characterization, evolutionary relationships, and family comparison.
- Citation du texte
- Dr. Prem Jose Vazhacharickal (Auteur), Sajeshkumar N.K (Auteur), Jiby John Mathew (Auteur), Anjana R (Auteur), Dona Ann Johns (Auteur), 2017, Molecular phylogenetic studies of pumpkin (Cucurbita argyrosperma) and winter melon (Benincasa hispida), Munich, GRIN Verlag, https://www.grin.com/document/351564
