Glyphosate (N-phosphonomethylglycine) is a herbicide that is used worldwidely. Its common trade name is Roundup. Its non-targeted species action makes it most popular herbicide. It was developed by Monsanto company. The primary target for glyphosate is the enzyme EPSPS (5-enolpyruvoylshikimate 3-phosphate synthase). When glyphosate binds to EPSPS it forms a very stable complex that essentially permanently disables the enzyme and hence affect the metabolic activity of the plant and results in its death. Finding Glyphosate degrading microorganisms from soil is an interesting topic since glyphosate is non- targeted in its toxicity. Microorganisms were isolated from soil samples, which were then identified by molecular method. Isolation of DNA, its amplification using 16s rRNA gene and its sequencing are the major steps involved. Bioinformatics tool helps to identify the microorganisms. Two microorganisms identified are Pseudomonas sp. and Achromobacter sp. In the phylogenetic analysis also the two organisms are grouped as separate clads. In these, strain 1 showed highest growth in the Glyphosate containing medium than strain 2. These results show that the bacterial strain may possess potential to be used in bioremediation of glyphosate-contaminated environments.
Inhaltsverzeichnis (Table of Contents)
- Isolation and molecular characterization of Glyphosate resistant bacteria from agricultural soils in Kerala
- Abstract
- Introduction
- Objectives
- Scope of the study
- Review of literature
- Hypothesis
- Materials and Methods
- Study area
- Soil sample collection
- Isolation of microorganisms from soil
- Morphological and biochemical tests
- Molecular identification and isolation of DNA
- Quantification of DNA
- Agarose gel electrophoresis
- PCR amplification using 16s rRNA
- Data sequencing
- Data analysis
- Glyphosate degradation
- Comparitive study for Glyphosate tolerance
- Statistical analysis
- Results and discussion
- Isolation of microorganism from soil
- Pure culture preparation, morphological and biochemical identification
- Bacterial DNA isolation
- PCR qmplification using 16s rRNA and sequencing
- Sequence analysis
- Molecular phylogentic analysis using maximum likelihood method
- Evaluation of Glyphosate degredation capacity
- Evaluation of Glyphosate tolerence capacity
- Conclusions
- Acknowledgements
- References
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This research project aims to isolate and characterize glyphosate-resistant bacteria from agricultural soils in Kerala, India. The study investigates the microbial communities present in these soils and explores their potential for bioremediation of glyphosate contamination.
- Isolation and identification of glyphosate-resistant bacteria from agricultural soils.
- Molecular characterization of the isolated bacteria using 16s rRNA sequencing.
- Evaluation of the glyphosate degradation capacity of the isolated bacteria.
- Assessment of the glyphosate tolerance levels of the isolated bacteria.
- Exploration of the potential for using these bacteria in bioremediation strategies.
Zusammenfassung der Kapitel (Chapter Summaries)
- Introduction: This chapter provides background information on glyphosate, its environmental impact, and the importance of bioremediation strategies. It also outlines the objectives and scope of the study.
- Review of Literature: This chapter reviews existing literature on glyphosate resistance in bacteria, bioremediation of glyphosate contamination, and the microbial communities present in agricultural soils.
- Materials and Methods: This chapter details the methodology used in the study, including soil sample collection, isolation and identification of bacteria, DNA extraction, PCR amplification, sequencing, and analysis of glyphosate degradation and tolerance.
- Results and Discussion: This chapter presents the findings of the study, including the isolation and identification of glyphosate-resistant bacteria, their molecular characterization, and their potential for bioremediation.
Schlüsselwörter (Keywords)
The key terms and concepts explored in this research include glyphosate resistance, bioremediation, agricultural soils, Kerala, 16s rRNA sequencing, microbial communities, glyphosate degradation, and glyphosate tolerance.
- Quote paper
- Dr. Prem Jose Vazhacharickal (Author), Sajeshkumar N.K (Author), Jiby John Mathew (Author), C K Anjana (Author), 2016, Isolation and molecular characterization of Glyphosate resistant bacteria from agricultural soils in Kerala, Munich, GRIN Verlag, https://www.grin.com/document/355561
