Studies on genetic relationships among locally cultivated Musaceae varieties in Kerala employing rbcL and matK gene using PCR technique and RFLP markers

Table of contents

Table of figures

Table of tables

List of abbreviations

Studies on genetic relationships among locally cultivated Musaceae varieties in Kerala employing rbcL and matK gene using PCR technique and RFLP markers

Abstract

1. Introduction
1.1 Objectives
1.2 Objectives of the study
1.3 Taxonomical classification

2. Review of literature
2.1 Uses and importance
2.2 Banana stem and flower
2.3 Banana Leaves:
2.4 Environmental status:
2.5 Molecular markers

3. Hypothesis

4. Materials and Methods
4.1 Study area
4.2 Sample collection
4.3 Description of the species
4.4 Species identification
4.3 Isolation of DNA
4.5 Quantification of DNA
4.6 PCR primers
4.7 PCR amplification
4.8 Data sequencing
4.9 Data analysis
4.11 Statistical analysis

5. Results and discussion
5.1 BLAST search for rbcL: sample A; Palayankodan
5.2 BLAST search for rbcL: sample B; Njalipoovan
5.3 BLAST search for rbcL: sample C; Robusta
5.4 BLAST search for matK: sample A; Palayankodan
5.5 BLAST search for matK : sample B; Njalipoovan
5.6 BLAST search for matK: sample C; Robusta
5.7 Multiple sequence alignment for partial rbcL gene for Musaceae
5.8 Multiple sequence alignment for partial matK gene for Musaceae
5.9 Phylogenetic tree based on partial sequence rbcL gene by UPGMA method
5.10 Phylogenetic tree based on partial sequence rbcL gene by Maximum likelihood method
5.11 Calculation of genetic distance using rbcL gene
5.12 Phylogenetic tree based on partial sequence matK gene by UPGMA method
5.13 Phylogenetic tree based on partial sequence matK gene by Maximum likelihood method
5.14 Calculation of genetic distance using matK gene

6. Conclusions

Acknowledgements

References