This study examines lavender which is also known as Lavendula officinalis Chaix syn. Lavendula angustifolia Mill. (Family Lamiaceae). It focusses on medicinal plants in general and medicinal effects of lavender. The study also presents several in vitro manipulations of lavender.
The genus lavender consists of 28-30 species. True lavender is commonly known as English lavender. Lavenders are mainly classified in four categories on the basis of origin, plant type and varieties.
Table of Contents
Chapter 1: Introduction
1.1 Introduction
1.2 Distribution:
1.3 Botanical Description:
1.4 Importance:
1.5 Conclusion
Chapter 2: Medicinal Plants
2.0 Medicinal Plants:
2.1 Traditional System of Medicine:
2.2 Over the counter (OTC) non-prescription items:
2.3 Phytopharmaceuticals:
2.4 Freeze –dried herbs:
2.5 Essential oils:
2.6 Medicinal and aromatic plants facing genetic erosion:
2.7 Importance of tissue culture:
Chapter 3: Phytochemical analysis of Lavendula oficinalis Chaix
3.1 Washing and sterilization of glassware and other instruments:
3.2 Preparation and sterilization of the nutrient media:
3.3 Source of plant material and its sterilization:
3.4 Aseptic transfer conditions
3.5 Inoculation and incubation
3.6 Sub culturing
3.7 Hardening procedure/Acclimatization
3.8 Chemical analysis
3.9 Data Analysis
Chapter 4: In vitro clonal propagation of Lavendula officinalis Chaix through shoot tip culture
4.1 Effect of cytokinins:
4.1.1 Effect of auxins:
4.1.2 Effect of auxin cytokinin combinations:
4.1.3 Acclimatization:
Chapter 5: Phytochemical analysis of Lavendula officinalis Chaix
5.1 In vivo and in vitro production of terpenoid constituents of Lavendula officinalis Chaix:
5.2 Discussion and Conclusion
Research Objectives and Themes
This handbook aims to provide a comprehensive guide on the medicinal properties, cultivation challenges, and micropropagation techniques of Lavendula officinalis Chaix, while addressing the need for conservation due to genetic erosion. The research focuses on establishing efficient clonal propagation methods through tissue culture and analyzing the phytochemical composition to compare the biosynthetic potential of in vitro raised plants versus those growing under natural conditions.
- Botany and traditional medicinal applications of lavender.
- Micropropagation protocols using shoot tip cultures and hormonal optimization.
- Chemical profiling and analysis of terpenoid constituents.
- Strategies for conservation and large-scale industrial cultivation.
Excerpt from the Book
3.1 Washing and sterilization of glassware and other instruments:
Good quality glassware was used throughout study. The glassware was soaked in detergent solution for 12 hours, rinsed in tap water to remove the detergent and finally rinsed in double distilled water to remove the last traces of the detergent. The previously used and contaminated glassware was first autoclaved and then soaked overnight in chromic acid so as to remove the stains. Rest of the procedures is same as mentioned above. The washed glassware was then finally sterilised by placing it in an oven (160-180oc) for 3-5 hours. The metal instruments such as forceps’, scalpels, scissors, pipette tips and non-absorbent cotton plugs were firstly sterilised by autoclaving at 121Oc and 15 lbs. Pressure for 15-20 minutes and the sterilised by exposure to hot dry air (160-180oc) for 3 hours in an oven. The sterilised items were allowed to cool before removal from oven for further use.
Instruments used during inoculations viz: forceps, scalpels, scissors, pipette etc. were surface sterilised by dipping in rectified spirit and flamed on burner in laminar air flow before each inoculation.
Summary of Chapters
Chapter 1: Introduction: Provides an overview of the taxonomy, distribution, botanical characteristics, and industrial importance of Lavendula officinalis.
Chapter 2: Medicinal Plants: Discusses the general landscape of medicinal plants, the traditional systems of medicine, and the growing economic role of essential oils and tissue culture.
Chapter 3: Phytochemical analysis of Lavendula oficinalis Chaix: Details the laboratory methodologies for sterilization, media preparation, tissue culture, and the chemical profiling of the plant samples.
Chapter 4: In vitro clonal propagation of Lavendula officinalis Chaix through shoot tip culture: Explores the experimental results regarding hormone applications, shoot multiplication rates, and successful acclimatization of plantlets.
Chapter 5: Phytochemical analysis of Lavendula officinalis Chaix: Compares the terpenoid content between in vivo and in vitro samples and presents a final discussion on the viability of these propagation techniques.
Keywords
Lavendula officinalis, tissue culture, micropropagation, essential oils, terpenoid, BAP, Kn, shoot tip culture, phytochemical analysis, clonal propagation, genetic erosion, medicinal plants, hormone optimization, aromatherapy, secondary metabolites.
Frequently Asked Questions
What is the primary objective of this handbook?
The handbook aims to document successful micropropagation methods for Lavendula officinalis to overcome the low rooting efficiency of conventional stem cuttings, ensuring high-quality clonal production for industrial use.
Which plant species does the research focus on?
The research is exclusively focused on Lavendula officinalis Chaix, commonly known as English lavender.
What scientific methods are utilized for propagation?
The authors utilize plant tissue culture techniques, specifically shoot tip culture, using Murashige and Skoog (MS) basal medium supplemented with various plant growth regulators like BAP, Kinetin, IAA, and IBA.
What is the significance of the chemical analysis conducted?
The chemical analysis, conducted via Headspace Gas Chromatography, serves to compare the quality and secondary metabolite profile (like cineole and limonene) of in vitro propagated plants against naturally grown plants to ensure the commercial and therapeutic equivalence.
How is the acclimatization of the plants managed?
Regenerated plantlets are transferred to a specific soil mixture of sand, clay, and vermiculite, and are maintained in a greenhouse environment with controlled humidity and temperature before being transferred to field conditions.
What are the major themes covered in this work?
The work covers botanical descriptions, traditional medicine, laboratory techniques for sterile plant cell culture, growth factor optimization, and comparative phytochemical profiling.
Why are blue pigments mentioned in the culture process?
During the in vitro growth phase, the authors observed the exudation of blue pigments into the agar medium, a phenomenon they correlated with specific hormonal combinations and stress responses observed in callus cultures.
How do environmental factors affect the terpenoid profile?
The study notes that the age of the plant and the growth conditions (natural vs. in vitro) significantly influence the accumulation of secondary metabolites such as 1,8-cineole and limonene, providing insights into optimal harvesting times.
What conclusion do the authors draw regarding tissue culture for lavender?
The authors conclude that tissue culture is a superior, viable, and rapid alternative to conventional vegetative propagation, allowing for the conservation of germplasm and the production of disease-free, high-yield clones.
- Citation du texte
- Nahida Tun Nisa (Auteur), Dr. Hamida Chisti (Auteur), 2017, Handbook of lavender as a medicinal plant, Munich, GRIN Verlag, https://www.grin.com/document/379399
