The study includes measurement of bee activity in collecting and producing honey, propolis and bee venom according to bee race, in a comparative study between Carniolan hybrid and Italian hybrid. For one year and study of some biological and chemical properties of some bee products (Honey, propolis and bee venom) and their effects on three types of cancer cells (Hep-G2, Caco-2 and MCF-7) in a comparative study with the effort of two drugs (5Fu and Cis-pt) in different concentrations. The study dealt with the following points: 1. Activity honeybee in collecting and producing honey, propolis and bee venom according to bee race. 2. Biological properties of some bee products and their effects on some cancer cells. 3. Chemical properties of some bee products.
Apitherapy is the art and science of making therapeutic use of products of honey bee, including honey, bee pollen, propolis, royal jelly, bees wax and bee venom. apitherapy is defined as api, meaning bee and therapy from the greek word therapia, which literally means "curing" or "healing". apitherapy is healing by using bee products. hippocrates, the greek physician known as the "father of medicine", recognized the hialing virtues of bee venom for treating arthritis and other joint problems. Since the ancient egyptians, cultures across the ages have revered the honey bee for its healing powers. Integrative medical apitherapy is the application of bee products like honey, pollen, royal jelly and propolis in healing therapies. apitherapy is an ancient alternative healing modality dating back several thousand years and used by hippocrates, mentioned by plato, and others throughout our history.
Table of Contents
I. Introduction
II – REVIEW OF LITERATURE
2.1. Honeybee activity in collecting and producing some bee products:
2.2. Biological properties of some bee products :
2.3.Chemical properties and ATR-FTIR :
III- MATERIALS AND METHODS
3.1. Measurements of bee activity:
3.2. Biological assays
3.3 Chemical assays and ATR-FTIR
IV- RESULTS AND DISCUSSION
4.1. Honeybee activity in collecting and producing some bee products:
4.3. Chemical properties and ATR –FTIR of some bee products
Research Objectives and Focus
The primary objective of this investigation is to integrate research expertise from the Department of Bee Research and the Department of Pharmacognosy to conduct a comparative study on Carniolan and Italian hybrid honeybee races. The study evaluates their activity in collecting honey, propolis, and bee venom, analyzes the biological and chemical properties of these products, and assesses their potential cytotoxic effects on specific cancer cell lines compared to standard chemotherapy drugs.
- Measurement of honeybee activity regarding the collection and production of honey, propolis, and bee venom based on race.
- Evaluation of the biological properties of these bee products, specifically their cytotoxic effects on Hep-G2, Caco-2, and MCF-7 cancer cells.
- Assessment of the chemical composition of the collected bee products using ATR-FTIR spectroscopy and other chemical assays.
- Comparative analysis of the efficacy of bee products against standard cancer treatments (5Fu and Cis-pt).
Excerpt from the Book
I. Introduction
Apitherapy is the art and science of making therapeutic use of products of honey bee, including honey, bee pollen, propolis, royal jelly, bees wax and bee venom. apitherapy is defined as api, meaning bee and therapy from the greek word therapia, which literally means “curing” or “healing.” apitherapy is healing by using bee products. hippocrates, the greek physician known as the "father of medicine", recognized the hialing virtues of bee venom for treating arthritis and other joint problems. Since the ancient egyptians, cultures across the ages have revered the honey bee for its healing powers.Integrative medical apitherapy is the application of bee products like honey, pollen, royal jelly and propolis in healing therapies . apitherapy is an ancient alternative healing modality dating back several thousand years and used by hippocrates, mentioned by plato, and others throughout our history.
Honey is a natural product containing a complex mixture of sugars,minerals, proteins, vitamins, organic acids, flavonoids, phenolic acids, enzymes and other phytochemicals. It contains antioxidant molecules such as flavonoids, phenolic acids, catalase, carotenoids, peroxidase and catalase (Gheldof and Engeseth, 2002). Documented biological activities of honey includes antioxidant, immuno-modulatory, cancer prophylactic and curative properties (Al-yahya et al., 2013). In addition, experimental evidence indicates that honey from variety of floral and geographical sources could exert several health-beneficial effects, whichincludes gastroprotective, hepatoprotective, reproductive, hypoglycemic, antioxidant, antihypertensive, antibacterial, anti-fungal and anti- inflammatory effects (Erejuwa et al., 2012). Honey contains about 200 substances including sugars, phenolic acids, flavonoids, amino acids, proteins, vitamins and enzymes (Wang and Li, 2011). Phenolic compounds are considered among the main constituents contributing to the antioxidant and other beneficial properties of honey (Stephens et al., 2010; Aljadi and Kamaruddin, 2004). Studies show that the great majority of the bioactive compounds in honey consist of molecules with phenolic structures, such as phenolic acids, flavonoids, procyanidins and anthocyanins (Küçük et al., 2007; Sahin et al., 2011; Tezcan et al., 2011).
Summary of Chapters
I. Introduction: Provides an overview of apitherapy, detailing the therapeutic history and composition of honey, bee venom, and propolis.
II – REVIEW OF LITERATURE: Examines existing studies on honeybee collection activities, biological properties of bee products, and chemical analysis methods including ATR-FTIR.
III- MATERIALS AND METHODS: Describes the experimental setup, including the selection of bee colonies, collection techniques, and methodologies for chemical and biological assays.
IV- RESULTS AND DISCUSSION: Presents findings on honeybee productivity, the cytotoxic impact of bee products on cancer cells, and chemical analysis results.
Keywords
Apitherapy, Honeybee, Carniolan hybrid, Italian hybrid, Propolis, Bee venom, Cytotoxicity, Cancer cells, MCF-7, Hep-G2, Caco-2, ATR-FTIR, Phenolic content, Melittin, Antioxidant activity
Frequently Asked Questions
What is the core focus of this research?
The research focuses on the collection and production of honey, propolis, and bee venom by two specific honeybee hybrids and the subsequent analysis of the biological and chemical properties of these products.
Which bee races were compared?
The study compares the Carniolan hybrid and the Italian hybrid honeybee races regarding their production and collection efficiency.
What is the primary goal of the study?
The primary goal is to investigate how race-dependent differences in collecting bee products influence their chemical composition and potential therapeutic applications, particularly in cancer treatment.
Which scientific methods were employed?
The researchers utilized analytical techniques such as HPLC, ATR-FTIR spectroscopy, and various colorimetric assays for antioxidant and chemical content analysis, alongside standard cell-based MTT assays for cytotoxic evaluation.
What does the study cover in the main section?
The main sections cover honey production yields, pollen analysis, bee venom quantity and components (like Melittin and Phospholipase A2), and the extraction and chemical characterization of propolis.
What are the key defining characteristics of this work?
Key characteristics include a focus on comparative apitherapy, specific chemical profiling using infrared spectroscopy, and the evaluation of bee product toxicity against liver, colon, and breast cancer cell lines.
How does the bee race impact propolis gathering?
The study found that Carniolan hybrid colonies were significantly more active in collecting propolis compared to Italian hybrid colonies throughout the year.
What role does Melittin play in bee venom?
Melittin is identified as a major toxic component (constituting approximately 50% of the dry matter) and is crucial for the cytolytic and anti-cancer effects of bee venom.
How is honey quality assessed in this study?
Quality is assessed through the measurement of various parameters including water content, total soluble solids, and the activity of enzymes such as invertase, diastase, and catalase.
- Arbeit zitieren
- Farid Badria (Autor:in), Hassan M. Fathy (Autor:in), Ashraf S. Fatehe (Autor:in), Mohamed G. Ghazy (Autor:in), 2017, Honey and Its Products. Chemical, Biological and Therapeutic Applications, München, GRIN Verlag, https://www.grin.com/document/512415
