The study generally aims to evaluate the probiotic effects of the different concentrations of "Pediococcus acidilactici" incorporated in the feeds of juvenile white shrimp ("Litopenaeus vannamei"), in terms of growth performance and survival. Specifically, the study is designed to determine which of the probiotic concentrations fed to the shrimps is the optimum concentration. Also, it conducts carcass analyses (moisture, lipid, protein and ash) to evaluate the nutritive potential of Pediococcus acidilactici as probiotics in the diets of juvenile Litopenaeus vannamei.
In this study, the use of lactic acid bacteria "Pediococcus acidilactici" was tested as probiotics incorporated in the feeds of juvenile white shrimp ("Litopenaeus vannamei"). "Pediococcus acidilactici" is a facultative anaerobe bacteria that displays antagonism against pathogens using lactic acid and secretion of bacteria-killing substances. It can also enhance immune responses towards infectious coccidioidal diseases.
"P. acidilactici" was also observed to alleviate vertebral column compression syndrome, elevate blood leucocyte levels, improve intestinal microvilli morphology and increase enterocyte endocytic activity in rainbow trout "Oncorhynchus mykiss" and improve weight gain, survival, feed conversion ratio and haemolymph total antioxidant status in shrimp.
Table of Contents
CHAPTER 1. INTRODUCTION
1.1.Background and Rationale
1.2.Significance of the Study
1.3.Objectives of the Study
CHAPTER 2. REVIEW OF RELATED LITERATURE
2.1. Biology of P. vannamei
2.2. Pacific White Shrimp Production
2.3. History and the Definition of Probiotics
2.4. Application of Probiotics in Aquaculture
2.4.1 Promoter of Growth Food utilization and Survival
2.4.2 Improvement of Water Quality
2.5. Selection of Probiotics
2.5.1 Modes of Probiotic Action
2.5.2 Probiotic Organism
2.6. Biology of Pediococcus acidilactici
2.7. Application of Pediococcus acidilactici as Probiotic
2.7.1 Livestock
2.7.2 Aquaculture
2.7.3 Inclusion in Feeds
CHAPTER 3. METHODOLOGY
3.1. Experimental Animals and Acclimatization
3.2. Experimental Design
3.3. Sampling and Water Quality Parameters
3.4. Experimental Feed Preparation
3.5. Feeding Trial
3.6. Proximate Composition of Experimental Diet and Shrimp Carcass
3.6.1. Moisture
3.6.2. Crude Protein
3.6.3. Crude Lipid
3.6.4. Ash
3.7. Statistical analysis
CHAPTER 4. RESULTS
4.1. Growth Performance
4.1.1 Average Body Weight
4.1.2 Percent Weight Gain
4.1.3 Specific Growth Rate
4.1.4 Feed Conversion Ratio
4.1.5 Protein Efficiency Ratio
4.1.6 Protein Productive Value
4.1.7 Survival Rate
4.2. Proximate Analyses of Experimental Diet and Shrimp Carcass
CHAPTER 5. DISCUSSION
5.1. Growth Performance
5.2. Proximate Analyses of Shrimp Carcass
CHAPTER 6. CONCLUSION AND RECOMMENDATION
Objectives & Scope
The primary objective of this study is to evaluate the effects of different concentrations of the probiotic Pediococcus acidilactici, when incorporated into the diet of juvenile Pacific white shrimp (Litopenaeus vannamei), on their growth performance and survival over a 75-day period, while also conducting carcass analysis to determine the nutritive potential of the probiotic supplementation.
- Assessment of various Pediococcus acidilactici concentrations (0%, 2%, 4%, 6%, and 10%).
- Evaluation of shrimp growth performance, including average body weight, weight gain, and specific growth rate.
- Monitoring of survival rates and feed conversion efficiency under experimental conditions.
- Proximate chemical analysis of shrimp carcass, specifically measuring moisture, protein, lipid, and ash content.
Excerpt from the Book
2.5. Selection of Probiotics
The selection of probiotic bacteria has been an empirical process based on limited scientific studies. One of the major cause of failure in probiotic research is the selection of inappropriate microorganisms. Probiotic bacteria needs to be adapted for different host species and environment. It is important to first understand and determine the mechanisms of probiotic action and to define selection criteria for potential probiotics. A potential probiotic should have the following characteristics: Capability of exerting beneficial effects on the host animal viz. increased growth or resistance to disease; Non-pathogenic and non-toxic to animals and human; Should be present as viable cells, preferably in large numbers although the minimum effective dose is not fully defined; Ability to withstand processing and storage; High tolerance to bile and gastric acid (low pH); Ability to adhere to epithelium or mucus; Persistency in intestinal tract; Ability to modulate immune response; Ability to produce inhibitory compounds; and Capability of altering microbial activity (Jadhav et al., 2015).
Summary of Chapters
CHAPTER 1. INTRODUCTION: Discusses the rapid growth of the global aquaculture industry, the challenges of infectious diseases, and the potential role of probiotics as an environmentally friendly alternative to traditional antimicrobial treatments.
CHAPTER 2. REVIEW OF RELATED LITERATURE: Provides an overview of the biology of Litopenaeus vannamei, the history and definition of probiotics, their applications in aquaculture, and the specific characteristics of Pediococcus acidilactici.
CHAPTER 3. METHODOLOGY: Details the experimental design, including the use of 15 glass aquaria, five triplicated treatments, and the specific procedures for feed preparation, feeding trials, and proximate analysis of shrimp carcass.
CHAPTER 4. RESULTS: Presents the findings of the study, noting that while there were no significant differences in general growth parameters, the 10% probiotic treatment showed a significant improvement in the protein productive value (PPV) and carcass composition.
CHAPTER 5. DISCUSSION: Analyzes the results in the context of existing literature, discussing factors such as stocking density, environmental stressors, and the specific mechanisms by which probiotic supplementation may influence nutrient absorption and immune response in shrimp.
CHAPTER 6. CONCLUSION AND RECOMMENDATION: Summarizes the study's findings, highlighting the significance of the observed differences in protein productive value, and provides recommendations for future research, including standardizing stocking densities and the microbiological verification of commercial probiotic supplements.
Keywords
Litopenaeus vannamei, Probiotic-supplemented diets, Pediococcus acidilactici, Growth Performance, Survival Rate, Proximate Analyses, Aquaculture, Feed efficiency, Protein Productive Value, Shrimp nutrition, Microbial intervention, Digestive enzyme activity, Disease resistance.
Frequently Asked Questions
What is the primary focus of this research?
This study investigates the effect of incorporating different concentrations of the lactic acid probiotic bacteria Pediococcus acidilactici into the diets of juvenile Pacific white shrimp to assess its impact on growth, survival, and carcass composition.
What are the central themes of the work?
The work focuses on sustainable aquaculture practices, the application of probiotics as feed additives, the growth performance of shrimp, and the proximate nutritional analysis of shrimp tissue under various dietary conditions.
What is the primary objective of the study?
The study aims to determine the optimal concentration of P. acidilactici in shrimp diets and to evaluate whether this probiotic enhances the nutritive potential and growth of juvenile L. vannamei.
What scientific methodology was employed?
The experiment utilized a 75-day feeding trial with five treatments in triplicated glass aquaria, followed by statistical analysis using one-way ANOVA to evaluate growth metrics and proximate composition parameters.
What does the main body of the work address?
The main body covers the literature review on shrimp biology and probiotic mechanisms, the detailed methodology of the feeding trial and laboratory analysis, the presentation of results through graphs and tables, and a discussion on why specific results occurred.
Which keywords best characterize this work?
Key terms include Litopenaeus vannamei, Pediococcus acidilactici, Probiotic-supplemented diets, Growth Performance, Survival Rate, and Proximate Analyses.
Did the probiotic significantly improve growth rates?
The results indicated no significant differences in the general growth performance parameters or survival rates between the treatment groups and the control at 75 days, though specific nutritional values improved.
What was the key observation regarding protein content?
A significant finding was that the Protein Productive Value (PPV) of shrimps fed with 10% probiotics was significantly higher compared to the other treatments and the control group.
- Citar trabajo
- Anonym (Autor), 2018, Juvenile Pacific white shrimp "Litopenaeus vannamei". Growth performance with lactic acid bacteria "Pediococcus acidilactici", Múnich, GRIN Verlag, https://www.grin.com/document/919535
