On the Effects of Garlic on the Total Cholestoral Level of Alloxan-Induced Diabetic Rats

Is Garlic Juice Able to Prevent the Complications of Diabetes?

Thèse de Bachelor , 2018 , 73 Pages , Note: 100

Chimie - Biologie Chimique

This study aims to research the effects of garlic on the total cholestoral level of alloxan-induced rats and tries to draw potential conclusions on the use of garlic juices in terms of preventing the complications of diabetes.

Diabetes has been implicated in as one of the major risk factors in the development of cardiovascular disease. In diabetic patient an increase in the serum lipid profile has been noticed. Several medicinal plants have been used in the treatment of diabetes. This research therefore focuses on the effect of garlic (Allium sativum) on total cholesterol of alloxan induced diabetic rats.

Twenty (20) Adult wistar rats were divided into four groups (n = 5): Group 1 served as the Control, Group 2 were administered with alloxan, Group 3 were administered with Alloxan+ Pioglitazone, Group 4 were administered with both Alloxan+ Allium sativum. After 4 week of experiment, all experimental animals were sacrificed. Blood was collected through eye puncture for the determination of Total cholesterol, HDL-Cholesterol, LDL-Cholesterol, Triglyceride and Fasting blood sugar.
In diabetic rats, elevated levels of Total cholesterol, LDL-Cholesterol, Triglyceride and Fasting blood sugar was noticed. The good cholesterol, HDL-Cholesterol, was, however, depleted. The abnormal alterations were significantly (p < 0.05) decreased in groups administered with Garlic and groups administered with standard drug when compared to Alloxan only group. However, the administration of Garlic led to a decrease in the level Total cholesterol, LDL-Cholesterol, Triglyceride and Fasting blood sugar in animals induced with diabetes.

These results indicate that garlic juice may help in the prevention of the complications of diabetes.

Inhaltsverzeichnis (Table of Contents)

• Title page
• Dedication
• Table of contents
• Abstract
• CHAPTER ONE
• 1.0 Introduction
• 1.1 Aim of research
• 1.2 Specific Objectives
• CHAPTER TWO
• 2.0 Literature Review
• 2.1 Allium Sativum
• 2.1.2 Propagation of Allium Sativum
• 2.1.3 Scientific classification of of Allium Sativum
• 2.1.4 Ecology Requirements and Production of Garlic
• 2.1.5 Growth and Development
• 2.1.6 Composition and Uses
• 2.1.7 Therapeutic effect of Garlic (Allium sativum L.)
• 2.1.8 Effects of garlic on cardiovascular diseases
• 2.1.9 Effects of garlic on Diabetes mellitus
• 2.1.10 Phytochemicals and method of Analysis in Allium Species
• 2.2 Diabetes
• 2.2.1 History of Diabetes
• 2.2.2 Pathophysiology of Diabetes
• 2.2.3 Complications of Diabetes
• 2.2.4 Diagnosis of Diabetes
• 2.2.5 Types of diabetes mellitus
• 2.2.6 Risk factors of Diabetes
• 2.2.7 Old and new approaches to treatment
• 2.3 Glucose
• 2.3.1 Glucose in Diabetes
• 2.4 Cholesterol
• 2.4.1 Chemical Structure of Cholesterol
• 2.4.2 Good Cholesterol and Bad Cholesterol
• 2.4.1 Hyperlipidemia
• CHAPTER THREE
• 3.0 Materials and Methods
• 3.1 Materials
• 3.1.1 Plant Materials
• 3.1.2 Apparatus and Equipments
• 3.1.3 Chemical Reagents
• 3.2 Methods
• 3.2.1 Plant Preparation
• 3.2.2 Management and Feeding of Animals
• 3.2.2.1 Feed composition
• 3.2.3 Grouping of Animals
• 3.2.4 Sacrifice and Sample Collection
• 3.2.5 Preparation of Samples
• 3.2.6 Determination of Total cholesterol
• 3.2.7 Determination of Triglyceride
• 3.2.8 Determination of Fasting Blood Sugar
• CHAPTER FOUR
• 4.0 Result
• CHAPTER FIVE
• 5.0 Discussion, Conclusion and Recommedation
• 5.1 Discusion
• 5.3 Recommendations
• REFERENCES

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This research aims to investigate the effects of garlic (Allium sativum) on total cholesterol levels in rats with alloxan-induced diabetes. The study seeks to determine if garlic can contribute to the management of hyperlipidemia in diabetic individuals. The primary goal is to evaluate the potential of garlic as a natural remedy for improving cholesterol levels in a diabetic model.

• The therapeutic effects of garlic on cardiovascular diseases and diabetes mellitus
• The impact of garlic on cholesterol levels in diabetic rats
• The phytochemicals present in Allium species and their potential for therapeutic applications
• The pathophysiology of diabetes and its associated complications
• The methods for analyzing and quantifying cholesterol and other relevant biochemical parameters

Zusammenfassung der Kapitel (Chapter Summaries)

Chapter One: Introduction introduces the research topic, highlighting the growing prevalence of diabetes and its associated complications, including hyperlipidemia. The chapter outlines the research objectives, specifically focusing on investigating the effects of garlic on total cholesterol levels in diabetic rats.

Chapter Two: Literature Review provides a comprehensive overview of existing knowledge related to garlic, diabetes, and cholesterol. It explores the medicinal properties of garlic, its effects on cardiovascular diseases and diabetes, and the phytochemicals responsible for its therapeutic effects. The chapter further discusses the pathophysiology of diabetes, its complications, and various treatment approaches.

Chapter Three: Materials and Methods details the experimental design and methodology employed in the study. This chapter describes the materials used, including plant materials, apparatus, and chemical reagents. It outlines the steps involved in animal management, sample collection, and biochemical analysis, specifically focusing on the determination of total cholesterol levels.

Chapter Four: Result presents the findings of the study. This chapter analyzes the data collected and provides statistical results regarding the effects of garlic on total cholesterol levels in diabetic rats.

Schlüsselwörter (Keywords)

The primary focus of this research revolves around the potential of garlic (Allium sativum) to modulate cholesterol levels in diabetic rats. Key concepts include diabetes mellitus, hyperlipidemia, phytochemicals, alloxan-induced diabetes, and biochemical analysis. The study employs empirical research methods to evaluate the therapeutic efficacy of garlic as a natural remedy for improving lipid profiles in a diabetic model.

• Cours: BIOCHEMISTRY
• Note: 100
• Auteur: Adeniyi Fajobi (Auteur)
• Année de publication: 2018
• Pages: 73
• N° de catalogue: V490807
• ISBN (ebook): 9783668999282
• ISBN (Livre): 9783668999299
• Langue: anglais
• mots-clé: effects, complications, prevent, able, juice, rats, diabetic, alloxan-induced, level, cholestoral, total, garlic, diabetes